Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,572,248 T→C 13.3% L124L (TTG→CTG) ‡ pcaB → 3‑carboxy‑cis,cis‑muconate cycloisomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,572,2480TC13.3% 110.7 / 8.3 45L124L (TTG→CTG) ‡pcaB3‑carboxy‑cis,cis‑muconate cycloisomerase
Reads supporting (aligned to +/- strand):  ref base T (18/21);  new base C (3/3);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

CAACTCGGCCATTCCGCTGGTGAAAGCGTTGGGCAAGGTGATCGCCACCGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGTCGCAGCAGGCCTTGAAGCACGCCGATACGCCCTTGGTGGGTCGTACCTGGTTGCAACACGCCACCCCGGTGACCCTGGGCATGAAA  >  NC_002947/1572103‑1572373
                                                                                                                                                 |                                                                                                                             
cAACTCGGCCATTCCGCTGGTGAAAGCGTTGGGCAAGGTGATCGCCACCGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGa                                                                                                                            <  7:157216/149‑1 (MQ=255)
  aCTCGGCCATTCCGCTGGTGAAAGCGTTGGGCAAGGTGATCGCCACCGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATc                                                                                                                          <  4:218902/149‑1 (MQ=255)
    tCGGCCATTCCGCTGGTGAAAGCGTTGGGCAAGGTGATCGCCACCGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATc                                                                                                                          >  3:28836/1‑147 (MQ=255)
        ccATTCCGCTGGTGAAAGCGTTGGGCAAGGTGATCGCCACCGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGcc                                                                                                                    <  7:38607/149‑1 (MQ=255)
              cGCTGGTGAAAGCGTTGGGCAAGGTGATCGCCACCGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTc                                                                                                              >  1:71711/1‑149 (MQ=255)
              cGCTGGTGAAAGCGTTGGGCAAGGTGATCGCCACCGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTc                                                                                                              >  6:185053/1‑149 (MQ=255)
              cGCTGGTGAAAGCGTTGGGCAAGGTGATCGCCACCGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTc                                                                                                              >  6:180997/1‑149 (MQ=255)
               gCTGGTGAAAGCGTTGGGCAAGGTGATCGCCACCGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCg                                                                                                             <  4:136813/149‑1 (MQ=255)
                cTGGTGAAAGCGTTGGGCAAGGTGATCGCCACCGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCgg                                                                                                            >  1:135523/1‑149 (MQ=255)
                   gTGAAAGCGTTGGGCAAGGTGATCGCCACCGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATCAAATCGAggg                                                                                                                     >  8:95549/1‑136 (MQ=255)
                   gTGAAAGCGTTGGGCAAGGTGATCGCCACCGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATCAAATCGAggg                                                                                                                     >  8:91708/1‑136 (MQ=255)
                   gTGAAAGCGTTGGGCAAGGTGATCGCCACCGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATCAAATCGAggg                                                                                                                     <  7:95549/137‑2 (MQ=255)
                   gTGAAAGCGTTGGGCAAGGTGATCGCCACCGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATCAAATCGAggg                                                                                                                     <  7:91708/137‑2 (MQ=255)
                     gAAAGCGTTGGGCAAGGTGATCGCCACCGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATCAAATCGAgg                                                                                                                      <  6:255496/134‑1 (MQ=255)
                     gAAAGCGTTGGGCAAGGTGATCGCCACCGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATCAAATCGAgg                                                                                                                      >  5:255496/1‑134 (MQ=255)
                      aaaGCGTTGGGCAAGGTGATCGCCACCGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCt                                                                                                      >  4:167324/1‑149 (MQ=255)
                        aGCGTTGGGCAAGGTGATCGCCACCGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCTgg                                                                                                    <  3:167324/149‑1 (MQ=255)
                                   aGGTGATCGCCACCGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCCAGCTTGCCGaggtcggc                                                                                         <  1:35425/149‑8 (MQ=255)
                                    ggTGATCGCCACCGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGt                                                                                        >  8:217510/1‑149 (MQ=255)
                                     gTGATCGCCACCGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGTc                                                                                       <  6:227424/149‑1 (MQ=255)
                                        aTCGCCACCGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGTCgca                                                                                    <  2:135523/149‑1 (MQ=255)
                                           gCCACCGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCgg                                                                                                            <  8:240590/122‑1 (MQ=255)
                                           gCCACCGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCgg                                                                                                            >  7:240590/1‑122 (MQ=255)
                                           gCCACCGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGTCgcagc                                                                                  <  2:71711/148‑1 (MQ=255)
                                                cGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGTCGCAGCAGGCCt                                                                            <  2:133076/149‑1 (MQ=255)
                                                   cGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGTCGCAGCAGGCCTTGa                                                                         <  5:99009/149‑1 (MQ=255)
                                                    gTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGTCGCAGCAGGCCTTGaa                                                                        <  4:28836/149‑1 (MQ=255)
                                                       ccAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGTCGCAGCAGGCCTTGAAGCa                                                                     >  4:222637/1‑149 (MQ=255)
                                                                 aGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGTCGCAGCAGGCCTTGAAGCACGCCGATa                                                             <  7:64450/147‑1 (MQ=255)
                                                                    gcTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGTCGCAGCAGGCCTTGAAGCACGCCGATACGccc                                                        >  1:210137/1‑149 (MQ=255)
                                                                        tgtgCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGTCGCAGCAGGCCTTGAAGCACGCCGATACGCCCTtgg                                                    <  6:69482/149‑1 (MQ=255)
                                                                          tgCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGTCGCAGCAGGCCTTGAAGCACGCCGATACGCCCTtggtg                                                  >  3:69258/1‑149 (MQ=255)
                                                                            cACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGTCGCAGCAGGCCTTGCAGCACGCCGATACGCCCTTGGTggg                                                >  8:219546/1‑149 (MQ=255)
                                                                            cACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGTCGCAGCAGGCCTTGAAGCACGCCGATACGCCCTtggtgg                                                 <  3:222637/148‑1 (MQ=255)
                                                                            cACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGTCGCAGCAGGCCTTGAAGCACGCCGATACGCCCTTGGTggg                                                >  7:107317/1‑149 (MQ=255)
                                                                            cACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGTCGCAGCAGGCCTTGAAGCACGCCGATACGCCCTTGGTggg                                                <  5:185053/149‑1 (MQ=255)
                                                                            cACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGTCGCAGCAGGCCTTGAAGCACGCCGATACGCCCTTGGTggg                                                <  5:180997/149‑1 (MQ=255)
                                                                                       caccaGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGTCGCAGCAGGCCTTGAAGCACGCCGATACGCCCTTGGTGGGTCGTACCTGGt                                     >  4:167350/1‑149 (MQ=255)
                                                                                        accaGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGTCGCAGCAGGCCTTGAAGCACGCCGATACGCCCTTGGTGGGTCATACCTGGtt                                    >  7:104373/1‑149 (MQ=255)
                                                                                          cagccagGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGTCGCAGCAGGCCTTGAAGCACGCCGATACGCCCTTGGTGGGTCGTACCTGGTTGc                                  <  3:167350/149‑1 (MQ=255)
                                                                                               agGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGTCGCAGCAGGCCTTGAAGCACGCCGATACGCCCTTGGTGGGTCGTACCTGGTTGCAacac                             >  7:8009/1‑149 (MQ=255)
                                                                                                      gATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGTCGCAGCAGGCCTTGAAGCACGCCGATACGCCCTTGGTGGGTCGTACCTGGTTGCAACACGCCAccc                      >  4:237853/1‑149 (MQ=255)
                                                                                                          gATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGTCGCAGCAGGCCTTGAAGCACGCCGATACGCCCTTGGTGGGTCGTACCTGGTTGCAACACGCCACCCCGGt                  <  3:237853/149‑1 (MQ=255)
                                                                                                                                          ccTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGTCGCAGCAGGCCTTGAAGCACGCCGATACGCCCTTGGTGGGTCGTACCTGGTTGCAACACGCCACCCCGGTGACCCTGGGCATGaaa  >  2:131474/1‑133 (MQ=255)
                                                                                                                                          ccTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGTCGCAGCAGGCCTTGAAGCACGCCGATACGCCCTTGGTGGGTCGTACCTGGTTGCAACACGCCACCCCGGTGACCCTGGGCATGaaa  <  1:131474/133‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                             
CAACTCGGCCATTCCGCTGGTGAAAGCGTTGGGCAAGGTGATCGCCACCGGCGTGCCAGAGGCTGAGCGCTATGTGCACCTTGGGGCCACCAGCCAGGATGCGATGGATACCGGTCTGGTTCTGCAGCTGCGCGATGCCCTCGATTTGATCGAGGCCGACCTCGGCAAGCTGGCCGATACCCTGTCGCAGCAGGCCTTGAAGCACGCCGATACGCCCTTGGTGGGTCGTACCTGGTTGCAACACGCCACCCCGGTGACCCTGGGCATGAAA  >  NC_002947/1572103‑1572373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑