Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,598,831 C→G 8.9% intergenic (‑70/+61) dctB ← / ← bglX C4‑dicarboxylate transport sensor protein/beta‑D‑glucoside glucohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,598,8310CG 8.9% 166.7 / 5.2 56intergenic (‑70/+61)dctB/bglXC4‑dicarboxylate transport sensor protein/beta‑D‑glucoside glucohydrolase
Reads supporting (aligned to +/- strand):  ref base C (29/22);  new base G (3/2);  total (32/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

CCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCGGCAACCC  >  NC_002947/1598704‑1598974
                                                                                                                                |                                                                                                                                               
ccAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGt                                                                                                                            >  6:226114/1‑149 (MQ=255)
  aGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGttt                                                                                                                          >  7:4888/1‑149 (MQ=255)
     aCGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTAc                                                                                                                       <  2:148697/149‑1 (MQ=255)
       gATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTAccc                                                                                                                     >  4:52413/1‑149 (MQ=255)
         tCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCcgc                                                                                                                   <  8:180834/149‑1 (MQ=255)
            gcagGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGa                                                                                                                 >  7:17801/1‑148 (MQ=255)
                    agcAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCg                                                                                                         <  8:243082/146‑1 (MQ=255)
                     tCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTTTTCGCGGGTAAAACCGCGCCTACAGGTGCTGCAGAGTACCTGTAGGCGCGGGTTTAc                                                                                                                       <  1:264359/133‑1 (MQ=21)
                     tCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTTTTCGCGGGTAAAACCGCGCCTACAGGTGCTGCAGAGTACCTGTAGGCGCGGGTTTAc                                                                                                                       >  2:264359/1‑133 (MQ=21)
                     tCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCgg                                                                                                        >  2:24861/1‑148 (MQ=255)
                     tCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCgg                                                                                                        >  3:219797/1‑148 (MQ=255)
                       aGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAAc                                                                                                     <  3:52413/149‑1 (MQ=255)
                          cccAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAgg                                                                                                  >  5:122033/1‑149 (MQ=255)
                           ccAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGt                                                                                                                            <  7:29367/122‑1 (MQ=255)
                           ccAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGt                                                                                                                            >  8:29367/1‑122 (MQ=255)
                           ccAGACACAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAggc                                                                                                 >  5:202908/1‑149 (MQ=255)
                            cAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGaa                                                                                                      <  7:120739/143‑1 (MQ=255)
                            cAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGaa                                                                                                      >  8:120739/1‑143 (MQ=255)
                             aGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAggcgg                                                                                               <  5:226114/149‑1 (MQ=255)
                             aGACACAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCTCGAAAAGGCCGGAACAggcgg                                                                                               <  6:202908/149‑1 (MQ=255)
                                   aGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGTCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTGCTGCAGAGTACCTGTAg                                                                                                                                   >  1:205570/1‑107 (MQ=25)
                                   aGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTGCTGCAGAGTACCTGTAg                                                                                                                                   <  2:205570/107‑1 (MQ=25)
                                      gcgcGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCa                                                                                      <  7:78427/149‑1 (MQ=255)
                                      gcgcGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCa                                                                                      <  4:219797/149‑1 (MQ=255)
                                          gAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTc                                                                                   >  7:197819/1‑148 (MQ=255)
                                            aaGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCaaa                                                                                <  8:197819/149‑1 (MQ=255)
                                             aGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAg                                                                               <  8:17801/149‑1 (MQ=255)
                                                aaaaGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACGCTGCAGCACCTGTAGGAGCGGTTTTACCCGCGAAAAGGCCGGAACAGCCGGCATTTTTCAGTCAAAGaaa                                                                            >  7:244153/1‑149 (MQ=38)
                                                 aaaGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGa                                                                                                       >  1:107720/1‑121 (MQ=255)
                                                       cATCGGGGGCGGATCCGGTGGTGGGATTGTCGGGGCCTGCCTCTTCCCGGGTAAACCCGCGCATACAGGTAATCTGCAGCCCCTTTAGGCGCGGTTTTACCCGCGAAAAGCCCGGAACAGCCGGCATTTTTAAAACAAACCAAtaaa                                                                        >  1:148586/1‑144 (MQ=255)
                                                       cATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTGCTCTGCCGCCCCGGGAGGCGCGGGGTTACCCGCGCAGAGGCAggcc                                                                                                      >  7:176797/1‑114 (MQ=25)
                                                         tCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTGTTTCCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGc                                                                    >  6:131869/1‑148 (MQ=255)
                                                                            tggGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCTCCTGTAGGCGCGGGTTTAACCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTAAAAGCTCTGCTGCTGCCCCGCCTgg                                                >  4:138878/1‑149 (MQ=38)
                                                                            tggGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTgg                                                <  6:122033/149‑1 (MQ=255)
                                                                              ggA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTggg                                               >  5:85110/1‑148 (MQ=255)
                                                                                           ctgactCTTCGCGGGTAATCCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCATTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTAc                                  <  8:244153/149‑1 (MQ=255)
                                                                                             gcctCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAg                                                                               >  1:44626/1‑102 (MQ=255)
                                                                                             gcctCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAg                                                                               <  2:44626/102‑1 (MQ=255)
                                                                                              cctCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTg                               >  8:200615/1‑149 (MQ=255)
                                                                                                     gcgGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCATCGCCTGGGAATCCAGCCCTACCTGGACAtt                         <  1:24861/148‑1 (MQ=255)
                                                                                                         gTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACt                    <  7:70876/149‑1 (MQ=255)
                                                                                                         gTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTCGCAATCCAGCCCTACCTGGACATTGAAc                     >  6:125670/1‑148 (MQ=255)
                                                                                                            aaCCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTccc                 >  1:60469/1‑149 (MQ=255)
                                                                                                               ccGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAg                                        <  3:180331/123‑1 (MQ=255)
                                                                                                               ccGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAg                                        >  4:180331/1‑123 (MQ=255)
                                                                                                               ccGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTg              >  3:67454/1‑149 (MQ=255)
                                                                                                                 gcgcCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGa                                              <  4:164738/115‑1 (MQ=255)
                                                                                                                 gcgcCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGa                                              >  3:164738/1‑115 (MQ=255)
                                                                                                                   gcCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTc          <  6:85110/149‑1 (MQ=255)
                                                                                                                    ccTACAGGTGCTGCAGAGTACCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCg         >  7:134776/1‑149 (MQ=37)
                                                                                                                     cTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCgg        >  8:74465/1‑149 (MQ=255)
                                                                                                                     cTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCgg        <  2:60469/149‑1 (MQ=255)
                                                                                                                           gTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAg                                                                     >  2:256847/1‑82 (MQ=255)
                                                                                                                           gTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAg                                                                     <  1:256847/82‑1 (MQ=255)
                                                                                                                           gTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCGGCAAccc  >  7:253542/1‑149 (MQ=255)
                                                                                                                           gTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCGGCAAccc  >  1:13079/1‑149 (MQ=255)
                                                                                                                                |                                                                                                                                               
CCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGA‑TGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCGGCAACCC  >  NC_002947/1598704‑1598974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑