Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,631,095 C→A 11.4% intergenic (+29/‑152) PP_1430 → / → lepA DegP‑like serine endoprotease/elongation factor 4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,631,0950CA11.4% 125.2 / 7.1 44intergenic (+29/‑152)PP_1430/lepADegP‑like serine endoprotease/elongation factor 4
Reads supporting (aligned to +/- strand):  ref base C (20/19);  new base A (3/2);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.19e-01

TCGGTTCGGCCAAGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCTTTTCGAGTGTT  >  NC_002947/1630948‑1631241
                                                                                                                                                   |                                                                                                                                                  
tCGGTTCGGCCAAGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCg                                                                                                                                                   >  4:156264/1‑149 (MQ=255)
          cAAGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCCGAAGCTGCCCttt                                                                                                                                         <  4:184176/149‑1 (MQ=255)
           aaGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCAAAAGGGCAGCCGAAGCTGCCCtttc                                                                                                                                        <  1:259946/149‑2 (MQ=255)
            aGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCttttt                                                                                                                                       <  3:156264/149‑1 (MQ=255)
               aaTTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCa                                                                                                                                     <  1:229541/148‑1 (MQ=255)
                      cGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCt                                                                                                                              >  6:40287/1‑148 (MQ=255)
                       gAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGTAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTc                                                                                                                            <  5:42240/149‑1 (MQ=255)
                        aaaTCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTc                                                                                                                            >  8:16353/1‑148 (MQ=255)
                        aaaTCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTc                                                                                                                            <  7:16353/148‑1 (MQ=255)
                             gCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAAtt                                                                                                                      >  5:92773/1‑149 (MQ=255)
                              ccAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTg                                                                                                                     >  1:8556/1‑149 (MQ=255)
                                aaGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGcc                                                                                                                   <  4:50918/149‑1 (MQ=255)
                                 aGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCg                                                                                                                  <  5:40287/149‑1 (MQ=255)
                                   gAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCgg                                                                                                                 >  4:151422/1‑148 (MQ=255)
                                          cGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTg                                                                                                         <  6:92773/149‑1 (MQ=255)
                                            aagaaTCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCt                                                                                                       <  7:82990/149‑1 (MQ=255)
                                             agaaTCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCtg                                                                                                      <  3:151422/149‑1 (MQ=255)
                                              gaaTCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTAGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCtgt                                                                                                     <  5:171270/149‑1 (MQ=255)
                                                 tCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTTTGTa                                                                                                  >  7:227681/1‑149 (MQ=255)
                                                        gtgtCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCg                                                                                            >  6:195421/1‑148 (MQ=255)
                                                              aTGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACagag                                                                                     >  1:4020/1‑149 (MQ=255)
                                                                gcgcGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAgg                                                                                    >  3:73539/1‑148 (MQ=255)
                                                                  gcgTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTc                                                                                                                                      <  6:257860/96‑1 (MQ=255)
                                                                  gcgTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTc                                                                                                                                      >  5:257860/1‑96 (MQ=255)
                                                                       cTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTccc                                                                            <  5:268050/149‑1 (MQ=255)
                                                                               gggCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCca                                                                    <  4:73539/149‑1 (MQ=255)
                                                                                  ccGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCcagca                                                                 <  5:195421/149‑1 (MQ=255)
                                                                                   cGTGCCAGCTTCATCACTTTGAAGGTTGCTGAATAAGCAGTTTCGCTAGGCGAATGGTCAGGTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCTGAATGATTGCTGTGTACAGTCGGTCAGAGGAATCTCCGATATCCctgcag                                                                <  4:218020/149‑1 (MQ=255)
                                                                                                tcaCTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAAt                                                   >  3:115794/1‑149 (MQ=255)
                                                                                                        aaGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGc                                            >  4:201785/1‑148 (MQ=255)
                                                                                                          gCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTAt                                         >  5:108496/1‑149 (MQ=255)
                                                                                                                       gCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTcggcgg                               <  8:211243/146‑1 (MQ=255)
                                                                                                                       gCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTcggcgg                               >  7:211243/1‑146 (MQ=255)
                                                                                                                       gCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCggcgggcg                            >  7:213126/1‑149 (MQ=255)
                                                                                                                              cGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCt                     >  6:45368/1‑149 (MQ=255)
                                                                                                                                cAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCg                   <  2:4020/149‑1 (MQ=255)
                                                                                                                                  atgaaaaaGGGCAGCCGAAGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCg                   >  7:204052/6‑147 (MQ=255)
                                                                                                                                  aGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCg                   >  7:83369/1‑147 (MQ=255)
                                                                                                                                  aGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCa                 <  4:115794/149‑1 (MQ=255)
                                                                                                                                    gaaaaaGGGCAGCCGAAGCTGCCGTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGc               >  5:55246/4‑149 (MQ=255)
                                                                                                                                      aaaaGGGCAGCCGAAGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTCTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGATCGCAGCCt             >  7:149/2‑149 (MQ=255)
                                                                                                                                          gggCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTTGGCGGGCGTCCGGCTCGCAGCCTTTTc         <  2:8556/149‑1 (MQ=255)
                                                                                                                                           ggCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCTTTTc         >  4:143409/1‑148 (MQ=255)
                                                                                                                                                 ttCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCTTTTCGAGTGtt  >  4:109982/1‑149 (MQ=255)
                                                                                                                                                   cGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCTTTTCGAGTGtt  <  8:213126/147‑1 (MQ=255)
                                                                                                                                                   cGGCTGCACTGTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGGAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCTTTTCGAGTGtt  <  8:227681/147‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCGGTTCGGCCAAGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCTTTTCGAGTGTT  >  NC_002947/1630948‑1631241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑