Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,639,565 A→G 7.7% intergenic (‑155/‑156) czcR‑III ← / → PP_1439 response regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,639,5650AG 7.7% 144.0 / 2.6 52intergenic (‑155/‑156)czcR‑III/PP_1439response regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (24/24);  new base G (2/2);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-01

GGTATGTTGAAAGGGGACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATG‑AAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAG  >  NC_002947/1639420‑1639711
                                                                                                                                                   |                                                                                                                                                  
ggTATGTTGAAAGGGGACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATG‑AAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGGGt                                                                                                                                                  >  7:96432/1‑148 (MQ=255)
 gTATGTTGAAAGGGGACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGt                                                                                                                                                  >  5:55884/1‑148 (MQ=255)
    tGTTGAAAGGGGACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATG‑AAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAAc                                                                                                                                              <  7:221424/148‑1 (MQ=255)
    tGTTGAAAGGGGACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATG‑AAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAAc                                                                                                                                              <  3:259581/148‑1 (MQ=255)
       tGAAAGGCGAAGATTCTTGACCCTGTAGTGAAATCAGGGTCAAGCGCCCCAATGTTACGGCATG‑AAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCcg                                                                                                                                           <  3:156616/148‑1 (MQ=255)
          atgtggACGATTCTTGTCGCTGTAGTGACATCAGGGGGAAGCGCCGCAATGTTACGGCATT‑AAAAAAGGCCTGCGGTTCAGAAAA‑CGGATTTGTAAATCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCcgctc                                                                                                                                        <  2:214312/144‑3 (MQ=255)
            ggggACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATG‑AAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTg                                                                                                                                     >  2:23019/1‑149 (MQ=255)
               gACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATG‑AAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAg                                                                                                                                  >  7:240175/1‑149 (MQ=255)
               gACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATG‑AAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAg                                                                                                                                  >  2:254418/1‑149 (MQ=255)
                      cTTGACCCTGATGTCACTACAGGGTCAAGCGCCCCAATGTTACGGCATG‑AAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACggg                                                                                                                           >  5:198697/1‑149 (MQ=255)
                       ttGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATG‑AAAAAAGGCCTGCGGTGCAGAT‑A‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGtt                                                                                                                         >  7:106482/1‑149 (MQ=255)
                         gACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGt                                                                                                                          <  6:55884/148‑1 (MQ=255)
                         gACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATG‑AAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTACGCGAGTAAACCCGCGCCTGTAGGAACggg                                                                                                                           <  1:23019/146‑1 (MQ=255)
                             cTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATG‑AAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGGGTAAACCCGTTCCTACAGGCGCGGGTTTActc                                                                                                                    <  8:96432/149‑3 (MQ=37)
                             cTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATG‑AAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTAccc                                                                                                                    >  2:75646/1‑149 (MQ=255)
                                tAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATG‑AAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGGGTAAACCCGTTCCTACAGGCGCGGGTTTACTcg                                                                                                                   <  5:222347/147‑1 (MQ=37)
                                tAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATG‑AAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCGGTAGGAACGGGTTTACCcgc                                                                                                                  >  4:204315/1‑148 (MQ=255)
                                     aCATCAGGGTCAAGCGCCCCAATGTTACGGCATG‑AAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAagag                                                                                                            <  1:254418/149‑1 (MQ=255)
                                        tCAGGGTCAAGCGCCCCAATGTTACGGCATG‑AAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGTTCCTACAGGCGCGGGTTTAc                                                                                                                      <  8:239048/136‑1 (MQ=37)
                                        tCAGGGTCAAGCGCCCCAATGTTACGGCATG‑AAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGTTCCTACAGGCGCGGGTTTAc                                                                                                                      >  7:239048/1‑136 (MQ=37)
                                            ggTCACGCGCCCCAATGTTACGTCAGC‑AAAAAAGGCCTGCGGTGCAGATAA‑CGTTTTTGTAATTCTGCTTTTATCTGGCTGTCTGTGCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGaa                                                                                                     <  8:217576/149‑1 (MQ=255)
                                                     ccccAATGTTACGGCATG‑AAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTg                                                                                                                                     <  6:266258/108‑1 (MQ=255)
                                                     ccccAATGTTACGGCATG‑AAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTg                                                                                                                                     >  5:266258/1‑108 (MQ=255)
                                                        cAATGTTACGGCATG‑AAAAACGCCCGGCGGTGCATATAA‑CGGATTTGTAATTCTGCTGTTATCGGGCTGGCTGTGCGGGCCTCTTCGCGAGTAACCCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACaa                                                                                         <  8:57892/149‑1 (MQ=255)
                                                         aaTGTTACGGCATG‑AAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGTCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGaa                                                                                                               <  5:276582/126‑1 (MQ=255)
                                                         aaTGTTACGGCATG‑AAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGaa                                                                                                               >  6:276582/1‑126 (MQ=255)
                                                          aTGTTACGGCATG‑AAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAgg                                                                                       <  4:62765/149‑1 (MQ=255)
                                                                 ggCATG‑AAAAAAGGCCTGCGGTGCAGATAANCGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCAACACAAGGc                                                                                      >  1:24510/1‑144 (MQ=255)
                                                                 ggCATG‑AAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGc                                                                                      <  2:24510/143‑1 (MQ=255)
                                                                    aTG‑AAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTg                                                                               >  8:25208/1‑147 (MQ=255)
                                                                                     ggTGCAGATAA‑CGGATTTGTAATTCGGGTGTTTTCTGGCTGTCTGTTCGGGCGTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTTCCCGCGAATAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGc                                                             <  2:222205/149‑1 (MQ=255)
                                                                                       tGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGc                                                                              >  8:83525/1‑130 (MQ=255)
                                                                                         cAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCa                                                         <  8:240175/149‑1 (MQ=255)
                                                                                          aGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGc                                                                                                          >  6:140263/1‑99 (MQ=255)
                                                                                          aGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGc                                                                                                          <  5:140263/99‑1 (MQ=255)
                                                                                          aGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCAc                                                        >  7:80271/1‑149 (MQ=255)
                                                                                             tAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCtt                                                     >  3:94202/1‑149 (MQ=255)
                                                                                               a‑CGGATTTGTAATGCTGCTGTAATCTGGCTGGCTGTTCGGGCCTCTGCGCGAGTAAACCCGCGCCTGTAGGACCGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGc                                                   <  3:127047/149‑1 (MQ=255)
                                                                                                  ggATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTAGCGAGTTAAACCGCGGCTGTAGGAACGGGTTTACACGCGAAGAGGCCCGAACAGTGCACACAAGGCCACAATGCAGGACAGTTATACCCGCCTAACCTTGCCg                                                 >  6:26208/1‑149 (MQ=255)
                                                                                                                  tgTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCACCGCCTCACCTTCCCGTTAGACAGCCCTCGc                                  >  4:40207/1‑148 (MQ=255)
                                                                                                                       tCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGAt                            >  6:119273/1‑149 (MQ=255)
                                                                                                                               tctgtTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCgtgt                    <  7:25208/149‑1 (MQ=255)
                                                                                                                               actgtTCGGGCCTCTTCGCGGGTAAACCCGTTCCTACAGGCGCGGGTTTACCCGCGAAGAGGCCCGCACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCACGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCgtgt                    >  8:60964/2‑149 (MQ=21)
                                                                                                                                   ttCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGGACGGGGTTACCCGCGAAGAGGCCCGAACAGTCCCCCCAAGGCCCCCTTGCAGGCCTGTAATCCCCGCCTCACCTTGCCGTTAGCCCGCCCTCGCGAGGCTGGCCGTGGCTGa                >  3:226205/1‑149 (MQ=37)
                                                                                                                                     cGGCCCTCTACGCGAGTAAACCCCCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGAt               <  3:204315/148‑1 (MQ=255)
                                                                                                                                      gggCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCg             <  4:94202/149‑1 (MQ=255)
                                                                                                                                       ggCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCgg            >  4:98602/1‑149 (MQ=255)
                                                                                                                                          ctctTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCgtgt                    >  4:88949/1‑138 (MQ=255)
                                                                                                                                          ctctTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCgtgt                    <  3:88949/138‑1 (MQ=255)
                                                                                                                                            ctTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGttat        >  1:127517/1‑148 (MQ=255)
                                                                                                                                                gcgAGTAAACCCGCGCCTGTAGGAAGGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGa   <  4:226205/149‑1 (MQ=255)
                                                                                                                                                 cgAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAg  <  3:98602/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGTATGTTGAAAGGGGACGATTCTTGACCCTGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATG‑AAAAAAGGCCTGCGGTGCAGATAA‑CGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAG  >  NC_002947/1639420‑1639711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑