Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,647,927 G→A 100% Y265Y (TAC→TAT)  oprB‑II ← carbohydrate‑selective porin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,647,9270GA100.0% 129.3 / NA 40Y265Y (TAC→TAT) oprB‑IIcarbohydrate‑selective porin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/19);  total (21/19)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTCGGACGCCAGCGAGGTGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAGTAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTCTGCTCGAA  >  NC_002947/1647783‑1648070
                                                                                                                                                |                                                                                                                                               
gTCGGACGCCAGCGAGGTGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGGCCTTGAGAACATCTTGTGCCTTGGCATTACTgtaatagt                                                                                                                                             <  5:110392/149‑1 (MQ=255)
gTCGGACGCCAGCGAGGTGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCGGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTgtaatagt                                                                                                                                             <  5:114650/149‑1 (MQ=255)
     aCGCCAGCGAGGTGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCg                                                                                                                                        >  8:188070/1‑149 (MQ=255)
         cAGCGAGGTGACCTGTTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCAc                                                                                                                                    <  8:222610/149‑1 (MQ=255)
         cAGCGAGGTGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCAc                                                                                                                                    >  4:3447/1‑149 (MQ=255)
         cAGCGAGGTGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCAc                                                                                                                                    >  1:34378/1‑149 (MQ=255)
                gTGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTc                                                                                                                             <  7:96298/149‑1 (MQ=255)
                 tGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTcc                                                                                                                            >  4:134055/1‑149 (MQ=255)
                     ctgctgctgGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCtt                                                                                                                        <  7:188070/149‑1 (MQ=255)
                           ctgGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCaa                                                                                                                    <  1:132320/147‑1 (MQ=255)
                             gggCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCt                                                                                                                         <  3:234158/140‑1 (MQ=255)
                             gggCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCt                                                                                                                         >  4:234158/1‑140 (MQ=255)
                                 gcCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCtt                                                                                                               >  2:246921/1‑146 (MQ=255)
                                 gcCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCtt                                                                                                               <  1:246921/146‑1 (MQ=255)
                                    aaTCCACTAGTGGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTTCTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGGCTTGGAt                                                                                                           <  4:7236/147‑1 (MQ=255)
                                    aaTCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGATGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATa                                                                                                          >  3:7236/1‑148 (MQ=255)
                                      tCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCAGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGt                                                                                                       <  1:207748/149‑1 (MQ=255)
                                           aaGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGt                                                                                                  <  3:134055/149‑1 (MQ=255)
                                             gCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTc                                                                                                 >  2:36543/1‑148 (MQ=255)
                                              ccGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCa                                                                                               <  3:36263/149‑1 (MQ=255)
                                              ccGTGCTTGCTCGAACTGCTGAGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTCTCAGTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCACGCCTTGGATACGTGGGGTcc                                                                                                >  5:272025/1‑148 (MQ=255)
                                                    ttgctCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAg                                                                                         >  2:215313/1‑149 (MQ=255)
                                                                            gcgcCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATTCGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTg                                                                 >  5:231161/1‑149 (MQ=255)
                                                                              gccgcTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTggg                                                               <  3:3447/149‑1 (MQ=255)
                                                                                 gcTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCg                                                                                     >  8:200247/1‑124 (MQ=255)
                                                                                     aGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCAGGGtgcgg                                                          <  2:34378/147‑3 (MQ=255)
                                                                                       ggcggcCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGtgccgctgc                                                      <  2:199323/149‑1 (MQ=255)
                                                                                                      gTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAAc                                       >  6:3213/1‑149 (MQ=255)
                                                                                                             tCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGcc                                >  6:164040/1‑149 (MQ=255)
                                                                                                                ttGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGa                             >  8:141947/1‑149 (MQ=255)
                                                                                                                ttGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGa                             >  7:229817/1‑149 (MQ=255)
                                                                                                                     aaCATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTc                          >  8:217337/1‑147 (MQ=255)
                                                                                                                     aaCATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGa                        <  5:3213/149‑1 (MQ=255)
                                                                                                                      aCATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGa                        <  4:106105/148‑1 (MQ=255)
                                                                                                                          cTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACGCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTg                   >  1:221613/1‑149 (MQ=255)
                                                                                                                             gtgCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGtt                    <  6:231161/145‑1 (MQ=255)
                                                                                                                              tgCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAg               >  1:99033/1‑149 (MQ=255)
                                                                                                                               gCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGACACCATTGCGGGATTCGAGGTTGGAAgg              >  4:138406/1‑149 (MQ=255)
                                                                                                                                ccTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAggg             <  2:221613/149‑1 (MQ=255)
                                                                                                                                  ttGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGtt           >  6:241040/1‑149 (MQ=255)
                                                                                                                                    ggCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGtt           <  7:217337/147‑1 (MQ=255)
                                                                                                                                           cTGTAATAGTATCCTGCACGATATTCCCCTTTCAAGCCTTGGATACGTTGCGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTCTGCTCGaa  <  8:229817/149‑1 (MQ=38)
                                                                                                                                                |                                                                                                                                               
GTCGGACGCCAGCGAGGTGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAGTAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTCTGCTCGAA  >  NC_002947/1647783‑1648070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑