Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,665,258 T→A 10.2% I204F (ATC→TTC)  PP_1459 ← CBS domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,665,2580TA10.2% 138.4 / 6.3 49I204F (ATC→TTC) PP_1459CBS domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (23/21);  new base A (2/3);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

CAGCGCTTCCAGGGTCAGCTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATCGAAGTCGTCGGCC  >  NC_002947/1665112‑1665400
                                                                                                                                                  |                                                                                                                                               
cAGCGCTTCCAGGGTCAGCTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGAGCCGATCGAACg                                                                                                                                               >  4:159266/1‑149 (MQ=255)
    gcTTCCAGGGTCAGCTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCtc                                                                                                                                           <  2:253432/149‑1 (MQ=255)
         cAGGGTCAGCTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGAGCCGATCGAACGCATCGTCGa                                                                                                                                      <  3:159266/149‑1 (MQ=255)
         cAGGGTCAGCTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGAGCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTTTGGCGGCTGACGATCAGCTGCTCGACGAGCCGATCGAACGCATCGTCGa                                                                                                                                      <  5:80441/149‑1 (MQ=255)
                aGCTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGAt                                                                                                                               <  4:259057/149‑1 (MQ=255)
                        cTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCa                                                                                                                      >  6:186316/1‑149 (MQ=255)
                               agcagGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGtt                                                                                                               <  4:36100/149‑1 (MQ=255)
                                       gCTGATCAGCTTGGTGTTGAGGATCGCTTCGAGCTGGTTGATGTCGTTGTGGTACACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCTCTTCGTTGCGCGGCa                                                                                                       >  1:229530/1‑149 (MQ=255)
                                         tGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACtt                                                                                                                   <  8:109561/135‑1 (MQ=255)
                                         tGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACtt                                                                                                                   >  7:109561/1‑135 (MQ=255)
                                            tCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAg                                                                                                   >  6:207256/1‑148 (MQ=255)
                                             cAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGa                                                                                                 >  2:161617/1‑149 (MQ=255)
                                                    gtgtTGAGGATCGCTTCGACCTGGTTGTTGTCGTTGTTGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGATGTTGATGCCAATCCACTTCGTTGCGCGGCACCAGGATGTCg                                                                                            <  1:161617/148‑1 (MQ=255)
                                                    gtgtTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGtt                                                                                          <  5:247510/149‑1 (MQ=255)
                                                    gtgtTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGt                                                                                           <  5:207256/148‑1 (MQ=255)
                                                       ttGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGa                                                                                        <  3:142704/148‑1 (MQ=255)
                                                         gAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACgg                                                                                     >  7:177623/1‑149 (MQ=255)
                                                             aTCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGAt                                                                                 <  8:177623/149‑1 (MQ=255)
                                                                 cTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGtt                                                                                                               <  3:231435/115‑1 (MQ=255)
                                                                 cTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGtt                                                                                                               >  4:231435/1‑115 (MQ=255)
                                                                  ttCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACg                                                                                      >  6:133560/1‑139 (MQ=255)
                                                                  ttCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACg                                                                                      <  5:133560/139‑1 (MQ=255)
                                                                     gACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGa                                                                                                                                      <  4:168083/89‑1 (MQ=255)
                                                                     gACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGa                                                                                                                                      >  3:168083/1‑89 (MQ=255)
                                                                           gTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACt                                                                   >  6:216925/1‑149 (MQ=255)
                                                                               aTGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCa                                                                <  1:105388/148‑1 (MQ=255)
                                                                                   cGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCg                                                           <  4:134650/149‑1 (MQ=255)
                                                                                          gTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCa                                                    <  5:186316/149‑1 (MQ=255)
                                                                                                                    tGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGt                          >  3:121752/1‑149 (MQ=255)
                                                                                                                      aCGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACg                                                                                      >  3:146459/1‑87 (MQ=255)
                                                                                                                      aCGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACg                                                                                      <  4:146459/87‑1 (MQ=255)
                                                                                                                        gATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCt                            <  5:146253/143‑1 (MQ=255)
                                                                                                                        gATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCt                            >  6:146253/1‑143 (MQ=255)
                                                                                                                            aGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCg                                                                                            >  6:231989/1‑75 (MQ=255)
                                                                                                                            aGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCg                                                                                            <  5:231989/75‑1 (MQ=255)
                                                                                                                              ctgctCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCAt                >  3:189350/1‑149 (MQ=255)
                                                                                                                                 ctCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCg                                                                                            <  8:125365/70‑1 (MQ=255)
                                                                                                                                 ctCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCg                                                                                            >  7:125365/1‑70 (MQ=255)
                                                                                                                                 ctCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGAGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATCg              >  5:109879/1‑148 (MQ=255)
                                                                                                                                 ctCGACGAGCCGATCGAACGCATCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAg                                        >  4:131687/1‑122 (MQ=39)
                                                                                                                                 ctCGACGAGCCGATCGAACGCATCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAg                                        <  3:131687/122‑1 (MQ=39)
                                                                                                                                  tCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATCGaa            >  8:78929/1‑149 (MQ=255)
                                                                                                                                    gacgaTGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGtt                         >  6:13150/1‑134 (MQ=255)
                                                                                                                                    gacgaTGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGtt                         <  5:13150/134‑1 (MQ=255)
                                                                                                                                       gaTGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATCGAAgtcgt       >  7:224195/1‑149 (MQ=255)
                                                                                                                                       gaTGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACAAGGATGTCGTTGACGGTGATCTTGTCAAGCGACTGCAGCCCGTCGAGCAGACACTGGCGAGGCGCTTAGTGCTGTTAATACTCGTCGAActcct       >  7:115418/1‑147 (MQ=255)
                                                                                                                                            gTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGa                                         >  6:103776/1‑110 (MQ=255)
                                                                                                                                            gTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGa                                         <  5:103776/110‑1 (MQ=255)
                                                                                                                                            gTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATCGAAGTCGTCGGcc  >  6:71271/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CAGCGCTTCCAGGGTCAGCTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCC‑ATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATCGAAGTCGTCGGCC  >  NC_002947/1665112‑1665400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑