| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,683,251 | C→G | 18.8% | intergenic (+60/‑171) | recJ → / → PP_1478 | ssDNA‑specific exonuclease RecJ/xenobiotic reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,683,251 | 0 | C | G | 18.8% | 85.2 / 11.6 | 37 | intergenic (+60/‑171) | recJ/PP_1478 | ssDNA‑specific exonuclease RecJ/xenobiotic reductase |
| Reads supporting (aligned to +/- strand): ref base C (17/13); new base G (2/5); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTGGAGTTGGCGTACAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTCCGGGCCGGATCAC > NC_002947/1683104‑1683395 | ggTGGAGTTGGCGTACAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCcgcg < 8:121336/149‑1 (MQ=255) ttGGCGTTCAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCGCGGGCGgcgc < 8:178791/148‑1 (MQ=255) cGTACAAGCTGGATGTGAACGAGTTTCGCTGTAATTAGAGTGTGCATTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTTCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCGCGGGCGGCGCTCGAt < 4:191013/149‑1 (MQ=38) gTACAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCGCGGGCGGCGCTCGAtt < 7:152851/149‑2 (MQ=255) aaGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCGATCTca > 8:185269/1‑148 (MQ=255) gAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCGCGGGCGGCGCTCGAt < 1:119896/133‑1 (MQ=255) gAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCGCGGGCGGCGCTCGAt > 2:119896/1‑133 (MQ=255) gTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCgcggcg < 8:15249/121‑1 (MQ=255) gTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCgcggcg > 7:15249/1‑121 (MQ=255) tttCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCg < 6:32640/124‑1 (MQ=255) tttCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCg > 5:32640/1‑124 (MQ=255) agagTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTc > 8:79598/1‑136 (MQ=255) agagTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTc < 7:79598/136‑1 (MQ=255) tgtgCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAGATCGAGCGC‑‑CGCGGGGGCGGCGCTCGAtttgt > 5:149641/1‑109 (MQ=255) tgtgCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAGATCGAGCGC‑‑CGCGCGGGCGGCGCTCGAtttgt < 6:149641/113‑5 (MQ=38) cAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCca < 2:24392/149‑1 (MQ=255) aGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGaa > 4:211528/1‑133 (MQ=255) aGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGaa < 3:211528/133‑1 (MQ=255) tGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGc > 8:214954/1‑120 (MQ=255) ggAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCGAAGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCCGCGAACCCCTCGCCATCCCACAGAACCTCCCCccacc > 1:80509/1‑148 (MQ=255) ggAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAAt > 5:201719/1‑149 (MQ=255) aGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCt > 3:207887/1‑149 (MQ=255) gCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTg < 7:185269/149‑1 (MQ=255) cgcgCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGt < 4:207887/149‑1 (MQ=255) gcgcTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCg < 7:196290/77‑1 (MQ=255) gcgcTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCg > 8:196290/1‑77 (MQ=255) gCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTaa > 2:103692/1‑149 (MQ=255) gCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTaa > 4:68546/1‑149 (MQ=255) cGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAAt < 3:68546/149‑1 (MQ=255) cGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAAt < 1:103692/149‑1 (MQ=255) tCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCGATCTCACAGGCGCTGGAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAAt > 1:84829/1‑147 (MQ=255) tCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAAt < 2:84829/147‑1 (MQ=255) cctgcctTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCAc > 6:20306/1‑149 (MQ=255) ggATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCa < 5:149073/148‑1 (MQ=255) ggATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAg > 1:242445/1‑149 (MQ=255) cTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCAc < 2:242445/149‑1 (MQ=255) caAATCGAGCGC‑‑CGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGCACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAgtgt > 8:75390/1‑149 (MQ=255) aaaTCGAGCGC‑‑CGCCCGCGCGGCGCTCGATCTCCCAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGCACCTCCCCCCACCAATCTGGGCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAgtggc > 7:217234/1‑147 (MQ=255) ccgcGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTCCGGGCCGGATCAc > 7:56807/1‑148 (MQ=255) | GGTGGAGTTGGCGTACAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGC‑‑CGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTCCGGGCCGGATCAC > NC_002947/1683104‑1683395 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |