Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,807,193 C→G 10.9% A292A (GCC→GCG)  pyrG → CTP synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,807,1930CG10.9% 130.5 / 7.2 46A292A (GCC→GCG) pyrGCTP synthase
Reads supporting (aligned to +/- strand):  ref base C (22/19);  new base G (2/3);  total (24/22)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

TACAAGATCCCGGGCGTGCTGCATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGC‑TTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGAAGACATCGAGAACCAGGGCACCAGCCTGCTC  >  NC_002947/1807047‑1807337
                                                                                                                                                   |                                                                                                                                                
tACAAGATCCCGGGCGTGCTGCATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGC‑TTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCAt                                                                                                                                                >  4:8943/1‑149 (MQ=255)
    aGATCCCGGGCGTGCTGCATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGC‑TTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTc                                                                                                                                            <  3:174392/149‑1 (MQ=255)
       tCCCGGGCGTGCTGCATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGC‑TTCGGCCTGCAGTGCAATGGCGCCGACCTGTACGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGc                                                                                                                                         <  7:171285/149‑1 (MQ=255)
         ccGGGCGTGCTGCATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGC‑TTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATGGCGATGGTcacttc                                                                                                                                       <  1:66053/149‑6 (MQ=255)
         ccGGGCGTGCTGCATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGC‑TTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCaa                                                                                                                                       <  1:129280/149‑1 (MQ=255)
                tgctgcATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGC‑TTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATg                                                                                                                                <  3:8943/149‑1 (MQ=255)
                    gcATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGC‑TTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGGAAGTACATGGAgc                                                                                                                            >  1:92069/1‑149 (MQ=255)
                    gcATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGC‑TTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAgc                                                                                                                            >  3:116868/1‑149 (MQ=255)
                         cTCAGGGCCTGGACGACTTCGTCGTCGAGCGC‑TTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGctgctg                                                                                                                       >  6:208243/1‑149 (MQ=255)
                                 cTGGACGACTTCGTCGTCGAGCGCTTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCg                                                                                                                 >  7:167034/1‑148 (MQ=255)
                                            cgtcgtcgAGCAC‑TTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTg                                                                                                     <  8:227121/148‑1 (MQ=255)
                                                 tcgAGCGC‑TTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGaa                                                                                               <  8:167034/149‑1 (MQ=255)
                                                   gAGCGC‑TTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGTGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAg                                                                                              <  5:208243/148‑1 (MQ=255)
                                                          ttCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAg                                                                                       >  2:41242/1‑149 (MQ=255)
                                                          ttCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAg                                                                                       >  5:16984/1‑149 (MQ=255)
                                                              gCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGa                                                                                        >  1:183871/1‑144 (MQ=255)
                                                              gCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGa                                                                                        <  2:183871/144‑1 (MQ=255)
                                                                        cAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATc                                                                          <  1:41242/148‑1 (MQ=255)
                                                                          aTGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGAGGATAGAAGCGATGAGCCACGCCGGCAGCAc                                                                        >  4:34559/1‑148 (MQ=255)
                                                                           tGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCAcc                                                                       >  6:177517/1‑148 (MQ=255)
                                                                               gcCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCg                                                                  >  2:130961/1‑149 (MQ=255)
                                                                                        tgcGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAg                                                           <  1:28400/145‑1 (MQ=255)
                                                                                        tCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAgg                                                          <  2:181685/148‑1 (MQ=255)
                                                                                        tCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAg                                                           <  6:16984/147‑1 (MQ=255)
                                                                                        tCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAg                                                           <  1:130961/147‑1 (MQ=255)
                                                                                           gAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGc                                                                                                                  >  1:66227/1‑89 (MQ=255)
                                                                                           gAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGc                                                                                                                  <  2:66227/89‑1 (MQ=255)
                                                                                             gTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAAcc                                                    >  2:89092/1‑149 (MQ=255)
                                                                                                ggACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTgc                                                 >  3:114617/1‑149 (MQ=255)
                                                                                                ggACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTgc                                                 >  2:127733/1‑149 (MQ=255)
                                                                                                  aCAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTgcgc                                               <  2:220306/149‑1 (MQ=255)
                                                                                                  aCAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAACTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTgcg                                                >  7:78514/1‑148 (MQ=255)
                                                                                                      ggtggtCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACa                                           >  8:142773/1‑149 (MQ=255)
                                                                                                           tCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGAt                                      <  1:89092/149‑1 (MQ=255)
                                                                                                              aTGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCg                                   <  3:34559/149‑1 (MQ=255)
                                                                                                              aTGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCg                                   <  5:177517/149‑1 (MQ=255)
                                                                                                                        cAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGAAGACATc                          >  1:147134/1‑148 (MQ=255)
                                                                                                                         aaCCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGAAGACATc                          >  4:233585/1‑147 (MQ=255)
                                                                                                                            ccGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGAAGACATCGAGaa                     >  2:79309/1‑149 (MQ=255)
                                                                                                                                     ttgccgACCATGGCGATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGaa                                >  8:3220/6‑129 (MQ=255)
                                                                                                                                     ttgccgACCATGGCGATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGaa                                <  7:3220/124‑1 (MQ=255)
                                                                                                                                     ttgccgACCATGGCGATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGaa                                <  6:58812/124‑1 (MQ=255)
                                                                                                                                     ttgccgACCATGGCGATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGaa                                >  5:58812/6‑129 (MQ=255)
                                                                                                                                     gAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGAAGACATCGAGAACCAGGGCAc            >  5:84924/1‑149 (MQ=255)
                                                                                                                                            ccATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGAAGACATCGAGAACCAGGGCACCAGCCTg     >  6:165820/1‑149 (MQ=255)
                                                                                                                                               tCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGAAGACATCGAGAACCAGGGCACCAGCCTGCTc  <  4:116868/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TACAAGATCCCGGGCGTGCTGCATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGC‑TTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGAAGACATCGAGAACCAGGGCACCAGCCTGCTC  >  NC_002947/1807047‑1807337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑