Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,842,287	A→G	100%	L836P (CTG→CCG)	gacS ←	sensor protein GacS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,842,287	0	A	G	100.0%	121.9 / NA	37	L836P (CTG→CCG)	gacS	sensor protein GacS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/19);  total (18/19)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCACCGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCAGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCTTGCCAGCGGCCAGGCGCAGGCCCTCTTCGGGGTCGAGTACCTTCAGTTCGTCGCCGTTGGTCAGCAGCTCGGCCTGCGCCTGCTGCGGTGTGCCCAGGCTCAGGCCGGT  >  NC_002947/1842142‑1842432                                                                                                                                                  |                                                                                                                                                  gCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCACCGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCcggca                                                                                                                                                <  2:176400/149‑1 (MQ=255)         gACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCACCGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCt                                                                                                                                         <  2:78408/148‑1 (MQ=255)             cGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCACCGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCTCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCa                                                                                                                                    <  1:249918/149‑1 (MQ=255)                 gCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCACCGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTc                                                                                                                                <  3:69453/149‑1 (MQ=255)                    cGTAACTGCGGCACGCCGCAATAGCGTGAGGCACCGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGc                                                                                                                             >  7:25792/1‑149 (MQ=255)                       tattGCGGCGTGCCGCAATAGCGTGAGGCACCGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCagcagc                                                                                                                          >  1:9370/4‑149 (MQ=255)                        aCTGCGGCACGCCGCAATAGCGTGAGGCACCGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCGGc                                                                                                                          <  5:89783/148‑1 (MQ=255)                        aCTGCGGCACGCCGCAATAGCGTGAGGCAACGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGATGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGAATCCAGGGAGGCCGGCAGCATGCTCAGCATCTAAGCGGc                                                                                                                          >  8:58827/1‑148 (MQ=255)                          tGCGGCACGCCGCAATAGCGTGAGGCACCGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCGGc                                                                                                                          >  7:220638/1‑146 (MQ=255)                               cACGCCGCAATAGCGTGAGGCACCGTTCAGACGGTGTACCTGCTAGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCGGCCAGATCTg                                                                                                                  <  1:157250/149‑1 (MQ=255)                                aCGCCGCAATAGCGTGAGGCACCGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGc                                                                                                                 <  3:172863/149‑1 (MQ=255)                                aCGCCGCAATAGCGTGAGGCACCGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGc                                                                                                                 <  3:140379/149‑1 (MQ=255)                                   ccgcAATAGCGTGAGGCACCGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCtt                                                                                                              <  4:177195/149‑1 (MQ=255)                                    cgcAATAGCGTGAGGCACCGTTCAGACGGTGTACCTGCTAGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCTGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCTTg                                                                                                             <  3:215417/149‑1 (MQ=255)                                         tAGCGTGAGGCACCGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCTTGCCAGc                                                                                                        >  7:145317/1‑149 (MQ=255)                                         tAGCGTGAGGCACCGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCTTGCCAGc                                                                                                        <  8:25792/149‑1 (MQ=255)                                              tGAGGCACCGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCAt                                                                                                                                            >  6:117935/1‑108 (MQ=255)                                              tGAGGCACCGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCAt                                                                                                                                            <  5:117935/108‑1 (MQ=255)                                                        ttCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCTTGCCAGCGGCCAGGCGCAGGcc                                                                                         >  8:133018/1‑149 (MQ=255)                                                             aCGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCg                                                                                                                            <  4:68239/109‑1 (MQ=255)                                                             aCGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCg                                                                                                                            >  3:68239/1‑109 (MQ=255)                                                                        gCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCTTGCCAGCGGCCAGGCGCAGGCCCTCTTCGGGGTCGAg                                                                          >  4:29884/1‑148 (MQ=255)                                                                              tCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCTTGCCAGCGGc                                                                                                     <  5:85376/115‑1 (MQ=255)                                                                              tCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCTTGCCAGCGGc                                                                                                     >  6:85376/1‑115 (MQ=255)                                                                                  gCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCTTGCCAGCGGCCAGGCGCAGGCCCTCTTCGGGGTCGAGTACCTTCAGtt                                                               >  6:47579/1‑149 (MQ=255)                                                                                  gCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCTTGCCAGCGGCCAGGCGCAGGCCCTCTTCGGGGTCGAGTACCTTCAGtt                                                               >  3:146982/1‑149 (MQ=255)                                                                                      tttCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCTTGCCAGCGGCCAGGCGCAGGCCCTCTTCGGGGTCGAGTACCTTCAGTtcgtc                                                           >  2:94484/1‑149 (MQ=255)                                                                                          ggTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCTTGCCAGCGGCCAGGCGCAGGCCCTCTTCGGGGTCGAGt                                                                         >  7:191752/1‑131 (MQ=255)                                                                                          ggTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCTTGCCAGCGGCCAGGCGCAGGCCCTCTTCGGGGTCGAGt                                                                         <  8:191752/131‑1 (MQ=255)                                                                                                           gcggcgCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCTTGCCAGCGGCCAGGCGCAGGCCCTCTTCGGGGTCGAGTACCTTCAGTTCGTCGCCGTTGGTCAGCAGCTCGgc                                      <  7:58827/149‑1 (MQ=255)                                                                                                                        cTTCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCTTGCCAGCGGCCAGGCGCAGGCCCTCTTCGGGGTCGAGTACCTTCAGTTCGTCGCCGTTGGTCAGCAGCTCGGCCTGCGCCtgctgc                         >  7:68292/1‑149 (MQ=255)                                                                                                                         ttCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCTTGCCAGCGGCCAGGCGCAGGCCCTCTTCGGGGTCGAGTACCTTCAGTTCGTCGCCGTTGGTc                                                 >  1:241847/1‑124 (MQ=255)                                                                                                                         ttCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCTTGCCAGCGGCCAGGCGCAGGCCCTCTTCGGGGTCGAGTACCTTCAGTTCGTCGCCGTTGGTc                                                 <  2:241847/124‑1 (MQ=255)                                                                                                                         ttCGCGGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCTTGCCAGCGGCCAGGCGCAGGCCCTCTTCGGGGTCGAGTACCTTCAGTTCGTCGCCGTTGGTCAGCAGCTCGGCCTGCGCCTGCTGCg                        <  8:220638/149‑1 (MQ=255)                                                                                                                             cgGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCTTGCCAGCGGCCAGGCGCAGGCCCTCTTCGGGGTCGAGTACCTTCAGTTCGTCGCCGTTGGTCAGCAGCTCGgcctgcgcctg                             >  3:239441/1‑140 (MQ=255)                                                                                                                             cgGTCCGTATCCAGGGAGGCCGGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCTTGCCAGCGGCCAGGCGCAGGCCCTCTTCGGGGTCGAGTACCTTCAGTTCGTCGCCGTTGGTCAGCAGCTCGgcctgcgcctg                             <  4:239441/140‑1 (MQ=255)                                                                                                                                             aGGCCGGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCTTGCCAGCGGCCAGGCGCAGGCCCTCTTCGGGGTCGAGTACCTTCAGTTCGTCGCCGTTGGTCAGCAGCTCGGCCTGCGCCTGCTGCGGTGTGCCCAGGCTCAGGCCg    <  3:29884/149‑1 (MQ=255)                                                                                                                                               gCCGGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCTTGCCAGCGGCCAGGCGCAGGCCCTCTTCGGGGTCGAGTACCTTCAGTTCGTCGCCGTTGGTCAGCAGCTCGGCCTGCGCCTGCTGCGGTGTGCCCAGGCTCAGGCCGGt  >  7:195014/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                  GCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTGAGGCACCGTTCAGACGGTGTACCTGCTCGATCAGGCCATTTCGGTCATCGGCTGCGCGGGCGGCGCGTATGGCTTCGCGGTCCGTATCCAGGGAGGCCAGCAGCATGCTCAGCATGTCAGCGGCCAGATCTGCCTTGCCAGCGGCCAGGCGCAGGCCCTCTTCGGGGTCGAGTACCTTCAGTTCGTCGCCGTTGGTCAGCAGCTCGGCCTGCGCCTGCTGCGGTGTGCCCAGGCTCAGGCCGGT  >  NC_002947/1842142‑1842432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑