Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,851,115 A→C 12.2% A67A (GCA→GCC)  relA → ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,851,1150AC12.2% 109.5 / 8.3 41A67A (GCA→GCC) relAATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base A (21/15);  new base C (3/2);  total (24/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

CATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTGCTCTTGAGGTAGAGAAAAAGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGA  >  NC_002947/1850973‑1851260
                                                                                                                                              |                                                                                                                                                   
cATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTGCTCTTGAGGTAGAGAAAAAGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTg                                                                                                                                               <  3:177656/149‑1 (MQ=255)
cATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTGCTCTTGAGGTAGAGAAAAAGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTg                                                                                                                                               >  2:217138/1‑149 (MQ=255)
   ggTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTGCTCTTGAGGTAGAGAAAAAGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGa                                                                                                                                             >  2:46354/1‑148 (MQ=255)
    gTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTGCTCTTGAGGTAGAGAAAAAGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCCTGCCTGGaag                                                                                                                                           <  3:7823/149‑2 (MQ=255)
    gTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTGCTCTTGAGGTAGAGAAAAAGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGaaa                                                                                                                                           >  5:246766/1‑149 (MQ=255)
                     gCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTGCTCTTGAGGTAGAGAAAAAGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCt                                                                                                                          <  8:137855/149‑1 (MQ=255)
                                        tCGCGCAGCGCTGAAAGAAGCCAGCGAGTTTGCTCTTGAGGTAGAGAAAAAGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGCCCAgg                                                                                                     >  7:41109/1‑149 (MQ=255)
                                                  cTGAAAGAAGCCTGCGAGTTTGCTCTTGAGGTAGAGAAAAAGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCtggt                                                                                           >  7:225024/1‑149 (MQ=255)
                                                            ccTGCGAGTTTGCTCTTGAGGTAGAGAAAAAGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTc                                                                                 <  8:225024/149‑1 (MQ=255)
                                                                       gCTCTTGAGGTAGAGAAAAAGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCACGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTc                                                                      >  4:24936/1‑149 (MQ=255)
                                                                         tctTGAGGTAGAGAAAAAGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGa                                                                                                                                    >  4:235240/1‑87 (MQ=255)
                                                                         tctTGAGGTAGAGAAAAAGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGa                                                                                                                                    <  3:235240/87‑1 (MQ=255)
                                                                         tctTGAGGTAGAGAAAAAGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCgg                                                                    >  3:144553/1‑149 (MQ=255)
                                                                                gTAGAGAAAAAGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCtg                                                                                             >  5:167511/1‑117 (MQ=255)
                                                                                gTAGAGAAAAAGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCtg                                                                                             <  6:167511/117‑1 (MQ=255)
                                                                                gTAGAGAAAAAGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTgcgc                                                               >  3:102658/1‑147 (MQ=255)
                                                                                 tAGAGAAAAAGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAg                                                            <  1:217138/149‑1 (MQ=255)
                                                                                    agaAAAAGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAggg                                                          >  6:159154/1‑148 (MQ=255)
                                                                                      aaaaaGGGCAACACGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGAGCGAGGGc                                                         >  6:34255/1‑147 (MQ=255)
                                                                                       aaaaGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCAAg                                                      <  6:246766/149‑1 (MQ=255)
                                                                                        aaaGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCAAgg                                                     <  3:24936/149‑1 (MQ=255)
                                                                                        aaaGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCAAgg                                                     <  1:46354/149‑1 (MQ=255)
                                                                                          aGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCAAGGTc                                                   <  5:159154/149‑1 (MQ=255)
                                                                                           gggCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCAAGGTCa                                                  >  4:150212/1‑149 (MQ=255)
                                                                                               aaCCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCAAGGTCACCCt                                              >  3:221734/1‑149 (MQ=255)
                                                                                                      ccAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCAAGGTCACCCTCGCCGAg                                       <  4:144553/149‑1 (MQ=255)
                                                                                                          gCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCAAGGTCACCCTCGCCGAGGTca                                   <  4:221734/149‑1 (MQ=255)
                                                                                                                cTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACATCTCAAGCTCGACAAGGTCTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCAAGGTCACCCTCGCCTAGGAcagc                                 >  1:212747/1‑147 (MQ=255)
                                                                                                                cTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCAAGGTCACCCTCGCCGAGGTcagccagc                             >  5:247097/1‑149 (MQ=255)
                                                                                                                    gCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCgg                           >  4:195011/1‑147 (MQ=255)
                                                                                                                    gCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCgg                           <  3:195011/147‑1 (MQ=255)
                                                                                                                     cGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGttt                        <  3:150212/149‑1 (MQ=255)
                                                                                                                                tCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCggtggt             >  2:215833/1‑149 (MQ=255)
                                                                                                                                 gcgatttCCAGGCCTGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGgtggtg            >  3:76563/6‑149 (MQ=255)
                                                                                                                                 gcgatttCCAGGCCTGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTAATCTACCGCTCGGTGCGCGAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGGCCggtggt             >  6:7535/6‑148 (MQ=255)
                                                                                                                                 gcgatttCCAGGCCTGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTAATCTACCGCTCGGTGCGCGAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCggtggt             <  5:7535/143‑1 (MQ=255)
                                                                                                                                 ccAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGgtggtg            >  4:5211/1‑149 (MQ=255)
                                                                                                                                 ccAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCAGTGCGCGAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGgtggtg            >  2:102741/1‑149 (MQ=255)
                                                                                                                                   aGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCaa                                                       <  4:171588/104‑1 (MQ=255)
                                                                                                                                   aGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCaa                                                       >  3:171588/1‑104 (MQ=255)
                                                                                                                                     tttCCAGGCCTGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCAAGGTCACCCTCGACGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTcc         >  7:203268/2‑148 (MQ=255)
                                                                                                                                          aggcaggcCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCAAGGTCACCCTCGCCGAGGTc                                    <  7:56974/116‑1 (MQ=255)
                                                                                                                                          aggcaggcCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCAAGGTCACCCTCGCCGAGGTc                                    >  8:56974/1‑116 (MQ=255)
                                                                                                                                           ggcaggcCTGGAAATCGCCGAAATTCGGGCTGAC‑‑CTCCAGCTCGACCAGGACTCCATGGGGGCTGCGGTCATCTTCCGCTCGGTGCGCGAGGGCAAGGGCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTGCAAGCTGa  <  4:10031/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
CATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTGCTCTTGAGGTAGAGAAAAAGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGAC‑‑CTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGA  >  NC_002947/1850973‑1851260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑