| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,892,228 | T→G | 12.9% | L157R (CTG→CGG) | PP_1697 → | transcriptional regulator, GntR family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,892,228 | 0 | T | G | 12.9% | 79.4 / 4.7 | 31 | L157R (CTG→CGG) | PP_1697 | transcriptional regulator, GntR family |
| Reads supporting (aligned to +/- strand): ref base T (17/10); new base G (2/2); total (19/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
TGCCGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTTGCTGCAACTGCCGG > NC_002947/1892087‑1892371 | tGCCGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGctgctg < 3:139649/146‑1 (MQ=255)tGCCGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGctgctg > 4:139649/1‑146 (MQ=255) ggCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTgg < 5:93986/149‑1 (MQ=255) ttCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCATACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCAGCGGCTGGaag > 6:229858/1‑138 (MQ=255) ttCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCATACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCAGCGGCTGGaag < 5:229858/140‑3 (MQ=255) tGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGAc > 2:244013/1‑131 (MQ=255) tGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAg > 6:44599/1‑149 (MQ=255) gCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAggg < 5:44599/149‑1 (MQ=255) cGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCCGCCGCGCCAAACCTTCCAGCAGCGGCTGGaagg < 8:33584/129‑4 (MQ=255) cGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCCGCCGCGCCAAACCTTCCAGCAGCGGCTGGaagg > 7:33584/1‑126 (MQ=255) aTTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGTAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCgctgc > 1:85236/1‑149 (MQ=255) catcatTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAgg < 5:255288/149‑1 (MQ=255) ttCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTc > 7:131641/1‑149 (MQ=255) acGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAg < 6:92075/136‑1 (MQ=255) acGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAg > 5:92075/1‑136 (MQ=255) tGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCg > 3:167000/1‑147 (MQ=255) ggCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAAc < 7:160844/149‑1 (MQ=255) gcgcCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAAcc < 6:128247/149‑1 (MQ=255) gcCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCt > 7:19975/1‑149 (MQ=255) aGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGAcc > 4:72968/1‑149 (MQ=255) gCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGAccg > 1:14508/1‑149 (MQ=255) cTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAg > 1:264915/1‑148 (MQ=255) cTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAg < 2:264915/148‑1 (MQ=255) tGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGt < 3:109679/81‑1 (MQ=255) tGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGt > 4:109679/1‑81 (MQ=255) tGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCg < 5:254443/149‑1 (MQ=255) gaagaaATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGa > 2:250059/1‑149 (MQ=255) ccGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCg > 5:61080/1‑149 (MQ=255) cAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTtgct > 7:212704/1‑149 (MQ=255) aaaCCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTtgctg > 4:28250/1‑149 (MQ=255) cTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGATCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTTGCTGCAAc > 4:242153/1‑149 (MQ=255) ttCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTTGCTGCAACt > 7:168756/1‑149 (MQ=255) gCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTTGCTGCAACTgccgg < 8:168756/149‑1 (MQ=255) | TGCCGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTTGCTGCAACTGCCGG > NC_002947/1892087‑1892371 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |