Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,892,529	C→G	15.0%	intergenic (+39/‑56)	PP_1697 → / → PP_1698	transcriptional regulator, GntR family/MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,892,529	0	C	G	15.0%	103.2 / 9.4	40	intergenic (+39/‑56)	PP_1697/PP_1698	transcriptional regulator, GntR family/MFS transporter
Reads supporting (aligned to +/- strand):  ref base C (15/19);  new base G (2/4);  total (17/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

GTCGTGATCGAGCGCCTGGCCCGCGATTACGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTCGGCGGCTGGTCGCTCGATGCGCTGGAAGTGCAG  >  NC_002947/1892386‑1892677                                                                                                                                                |                                                                                                                                                     gtCGTGATCGAGCGCCTGGCCCGCGATTACGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACgg                                                                                                                                                 <  6:261992/149‑1 (MQ=255)    gTGATCGAGCGCCTGGCCCGCGATTACGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGGCAGCACGgcgc                                                                                                                                              <  3:9697/149‑1 (MQ=255)          gAGCGCCTGGCCCGCGATTACGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGGCAGCACGGCGCAgaca                                                                                                                                         <  1:256309/148‑4 (MQ=255)          gAGCGCCTGGCCCGCGATTACGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGc                                                                                                                                        >  2:113883/1‑149 (MQ=255)                  ggCCCGCGATTACGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACg                                                                                                                                                  >  4:209170/1‑131 (MQ=255)                  ggCCCGCGATTACGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACg                                                                                                                                                  <  3:209170/131‑1 (MQ=255)                   gCCCGCGATTACGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCATTGGCTTCGCCTGc                                                                                                                               <  8:95079/149‑1 (MQ=255)                   gCCCGCGATTACGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGc                                                                                                                               <  4:77630/149‑1 (MQ=255)                         gATTACGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTca                                                                                                                         <  8:141670/149‑1 (MQ=255)                                  ggCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGa                                                                                                                <  1:113883/149‑1 (MQ=255)                                        ccTCTGGAATGGCTCGGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGaa                                                                                                          <  4:190197/149‑1 (MQ=255)                                         ctctGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAg                                                                                                         >  1:198040/1‑149 (MQ=255)                                          tctGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGtgctgc                                                                                                                                                      <  1:154469/103‑1 (MQ=255)                                          tctGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGtgctgc                                                                                                                                                      >  2:154469/1‑103 (MQ=255)                                          tctGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCacac                                                                                                                      >  2:182229/1‑135 (MQ=255)                                          tctGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCacac                                                                                                                      <  1:182229/135‑1 (MQ=255)                                                   gcgcCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACACACGACTAGAAGGATAACAAt                                                                                                >  8:188439/1‑148 (MQ=255)                                                    cgccgcTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCa                                                                                              <  5:77183/149‑1 (MQ=255)                                                     gccgcTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGc                                                                                                                                   >  3:242834/1‑111 (MQ=255)                                                     gccgcTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGc                                                                                                                                   <  4:242834/111‑1 (MQ=255)                                                      ccgcTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATg                                                                                            >  2:61965/1‑149 (MQ=255)                                                       cgcTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGt                                                                                           <  8:156634/149‑1 (MQ=255)                                                          tCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCAACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGtt                                                                                          >  8:198285/1‑147 (MQ=255)                                                              gcgGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTg                                                                                    >  1:65203/1‑149 (MQ=255)                                                               cgGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTgg                                                                                   <  3:97281/149‑1 (MQ=255)                                                                        gcagcaCTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATcgc                                                                             >  8:174093/1‑146 (MQ=255)                                                                        gcagcaCTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCgag                                                                          <  7:198285/149‑1 (MQ=255)                                                                         cagcaCTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCCTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAg                                                                                                                                            <  2:19535/82‑1 (MQ=255)                                                                         cagcaCTTCCGCTACCGCATCGACATTCGATAAACCGGTCGCCTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAg                                                                                                                                            >  1:19535/1‑82 (MQ=255)                                                                           gcaCTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATc                                                                       <  5:144694/149‑1 (MQ=255)                                                                            caCTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCa                                                                      <  2:198040/149‑1 (MQ=255)                                                                               ttCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCt                                                                   >  2:239418/1‑149 (MQ=255)                                                                                   gCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACg                                                               >  2:251450/1‑149 (MQ=255)                                                                                    cTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTGGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACgg                                                              >  1:85192/1‑149 (MQ=255)                                                                                       ccGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCgaga                                                                         <  6:118447/135‑1 (MQ=255)                                                                                       ccGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCgaga                                                                         >  5:118447/1‑135 (MQ=255)                                                                                              gagaTTCGTTAATCCGGTCGCTTCACCCTTCACCTGTCTGCGACGTGCTGCAACGGCGCAGTGGCTTCGCCTGCCCGTAACACCCACGAATAGCAGGATAACAATCATGTTCAGCTGGTCGCTCGAGATCACCTCACGGGAACGCAAg                                                     <  5:187837/145‑1 (MQ=255)                                                                                                         aaCCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGc                                          <  4:257847/148‑1 (MQ=255)                                                                                                                                   cTGCGCCGTGGCAGCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTcgg                                 <  4:103679/131‑1 (MQ=255)                                                                                                                                   cTGCGCCGTGGCAGCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTcgg                                 >  3:103679/1‑131 (MQ=255)                                                                                                                                   cTGCGCCGTGGCAGCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTcgg                                 >  2:20784/1‑131 (MQ=255)                                                                                                                                   cTGCGCCGTGGCAGCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTcgg                                 <  1:20784/131‑1 (MQ=255)                                                                                                                                              tgcCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTCGGCGGCTGGTCGCTCGATGCGCTGGAAGTg     >  3:205644/1‑148 (MQ=255)                                                                                                                                                ccACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTCGGCGGCTGGTCGCTCGATGCGCTGGAAGTGCa   <  4:205644/148‑1 (MQ=255)                                                                                                                                                ccACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTCGGCGGCTGGTCGCTCGATGCGCTGGAAGTGCAg  <  7:174093/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                     GTCGTGATCGAGCGCCTGGCCCGCGATTACGCCGGCAAACCTCTGGAATGGCGCCGCTCGCGCGGCCATGCGCAGCACTTCCGCTACCGCATCGACATTCGTTAAACCGGTCGCTTCACCCTTCACCTGTCTGCGCCGTGCTGCCACGGCGCAGTGGCTTCGCCTGCCCGTCACACCCACGACTAGAAGGATAACAATCATGTTCAGCTGGTATCGCGAGATCACCTCACGGGAACGCAAGACATTCTGGGCCTGTTTCGGCGGCTGGTCGCTCGATGCGCTGGAAGTGCAG  >  NC_002947/1892386‑1892677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑