Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,921,549 T→A 18.6% intergenic (+24/+37) PP_1720 → / ← PP_1721 alcohol dehydrogenase/phosphoserine phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,921,5490TA18.6% 88.0 / 15.7 43intergenic (+24/+37)PP_1720/PP_1721alcohol dehydrogenase/phosphoserine phosphatase
Reads supporting (aligned to +/- strand):  ref base T (12/23);  new base A (5/3);  total (17/26)
Fisher's exact test for biased strand distribution p-value = 2.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.27e-01

GGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGG  >  NC_002947/1921404‑1921687
                                                                                                                                                 |                                                                                                                                          
ggTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTAc                                                                                                                                         <  1:184757/149‑1 (MQ=255)
       gccgcAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGTTACCAACCAGGTGTCGGGCAAGCTGGTGATTGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGaa                                                                                                                                  <  1:82796/149‑1 (MQ=255)
       gccgcAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCCGGCCCCTTCGCGGGTAAACCCGCGaa                                                                                                                                  <  1:72406/149‑1 (MQ=255)
                       acCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCGGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGccc                                                                                                                  <  6:243384/149‑1 (MQ=255)
                         cTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCa                                                                                                                 >  4:240089/1‑148 (MQ=255)
                                   gAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTc                                                                                                      <  2:188028/149‑1 (MQ=255)
                                   gAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCACGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTc                                                                                                      <  7:6447/149‑1 (MQ=255)
                                    aGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTca                                                                                                     <  1:172394/149‑1 (MQ=255)
                                     gTTTGCCCTGGCGGCGTAGGCCGCGCTGTAGACCACCCAGGTTTCGGGCAAGCTGGTGATGGTTATCGACCCTAGCCTGGTGTATGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTca                                                                                                     <  4:180262/148‑1 (MQ=255)
                                     gTTTGCCCAGGCGGCGTATGCGTAGCTGTAGACCAACCTGGTGTCGGGCAAGGTGGTTATGGTGATCGACCCTAGCCTGGTGTACGGGGGGCTGGCCCTTTCGCGGTTTTACCCGCTAAGGGGCCGGCACAGCCCATAAAAACCTTca                                                                                                     <  8:161028/148‑1 (MQ=255)
                                     gTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTcag                                                                                                    <  6:206193/149‑1 (MQ=255)
                                        tGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTCGTGTAAGCGGTGCTGGCCCCTTCGCGGGTTTACACGCGAAGGGGCCGGCACAGCCCATAAAAAACTTcaccc                                                                                                  >  8:124923/1‑145 (MQ=255)
                                               gcggcgTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGcac                                                                                                                       >  3:73379/1‑120 (MQ=255)
                                               gcggcgTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGCGGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCa                                                                                          <  2:63232/149‑1 (MQ=255)
                                                         ccGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCa                                                                                <  7:133872/149‑1 (MQ=255)
                                                          cGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCAc                                                                               <  6:266323/149‑1 (MQ=255)
                                                                 gagaCCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGa                                                                        >  7:153714/1‑149 (MQ=255)
                                                                 gagaCCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGa                                                                        >  7:149751/1‑149 (MQ=255)
                                                                       aaccaGGTGTCGGGCAAGCTTGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGTTTTACCGGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCATAGTCGGCGACCCATTGATCTCgg                                                                  <  5:201487/149‑1 (MQ=255)
                                                                        accaGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGc                                                                 <  8:153714/149‑1 (MQ=255)
                                                                        accaGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGc                                                                 <  8:149751/149‑1 (MQ=255)
                                                                          caGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCAt                                                               >  8:71145/1‑149 (MQ=255)
                                                                                 cGGGCAAGCTGGTGATGGTGAGCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCGTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTcgcg                                                        <  7:10514/149‑1 (MQ=255)
                                                                                  gggCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCgcgc                                                       <  6:70243/149‑1 (MQ=255)
                                                                                    gCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCaa                                                     >  3:126178/1‑149 (MQ=255)
                                                                                                                     tgtAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATc                    <  3:240089/149‑1 (MQ=255)
                                                                                                                          gCGGGTCTGGCCCCTTCGCGGTTTACCCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCActc               <  3:155380/149‑1 (MQ=255)
                                                                                                                          gCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCgg                          <  5:112761/138‑1 (MQ=255)
                                                                                                                          gCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCgg                          >  6:112761/1‑138 (MQ=255)
                                                                                                                                  ggCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTcaac          >  4:62460/1‑146 (MQ=255)
                                                                                                                                  ggCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTcaac          <  3:62460/146‑1 (MQ=255)
                                                                                                                                   gCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCAGTGATCTCGGCATGCTCGCGCAACCCTGCGTCCGGGTTCCCCACATGCGGGTGATCCCCTCTccacaac       >  3:245209/1‑148 (MQ=255)
                                                                                                                                   gCCCCTTCGCGGGTAAACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTcaacaac       >  2:100126/1‑148 (MQ=255)
                                                                                                                                     cccTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCgg                                         <  3:237450/112‑1 (MQ=255)
                                                                                                                                     cccTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCgg                                         >  4:237450/1‑112 (MQ=255)
                                                                                                                                      ccTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACg   <  7:71145/149‑1 (MQ=255)
                                                                                                                                       cTTCGCGGGTAAACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACt                                                 <  3:67058/102‑1 (MQ=255)
                                                                                                                                       cTTCGCGGGTAAACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACt                                                 >  4:67058/1‑102 (MQ=255)
                                                                                                                                        ttCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACgg  >  4:116366/1‑148 (MQ=255)
                                                                                                                                        ttCGCGGGTAAACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCTGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACgg  >  2:206098/1‑148 (MQ=255)
                                                                                                                                        ttCGCGGGTAAACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTcaa           <  7:136316/139‑1 (MQ=255)
                                                                                                                                        ttCGCGGGTAAACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTcaa           >  8:136316/1‑139 (MQ=255)
                                                                                                                                        ttCGCGGGTAAACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACgg  >  2:3674/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                          
GGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGG  >  NC_002947/1921404‑1921687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑