Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,921,550 T→A 21.0% intergenic (+25/+36) PP_1720 → / ← PP_1721 alcohol dehydrogenase/phosphoserine phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,921,5500TA21.0% 72.8 / 16.0 43intergenic (+25/+36)PP_1720/PP_1721alcohol dehydrogenase/phosphoserine phosphatase
Reads supporting (aligned to +/- strand):  ref base T (12/22);  new base A (5/4);  total (17/26)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

GGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGGCAAGTCATTCC  >  NC_002947/1921404‑1921698
                                                                                                                                                  |                                                                                                                                                    
ggTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTAc                                                                                                                                                    <  1:184757/149‑1 (MQ=255)
       gccgcAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGTTACCAACCAGGTGTCGGGCAAGCTGGTGATTGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGaa                                                                                                                                             <  1:82796/149‑1 (MQ=255)
       gccgcAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCCGGCCCCTTCGCGGGTAAACCCGCGaa                                                                                                                                             <  1:72406/149‑1 (MQ=255)
                       acCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCGGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGccc                                                                                                                             <  6:243384/149‑1 (MQ=255)
                         cTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCa                                                                                                                            >  4:240089/1‑148 (MQ=255)
                                   gAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTc                                                                                                                 <  2:188028/149‑1 (MQ=255)
                                   gAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCACGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTc                                                                                                                 <  7:6447/149‑1 (MQ=255)
                                    aGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTca                                                                                                                <  1:172394/149‑1 (MQ=255)
                                     gTTTGCCCTGGCGGCGTAGGCCGCGCTGTAGACCACCCAGGTTTCGGGCAAGCTGGTGATGGTTATCGACCCTAGCCTGGTGTATGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTca                                                                                                                <  4:180262/148‑1 (MQ=255)
                                     gTTTGCCCAGGCGGCGTATGCGTAGCTGTAGACCAACCTGGTGTCGGGCAAGGTGGTTATGGTGATCGACCCTAGCCTGGTGTACGGGGGGCTGGCCCTTTCGCGGTTTTACCCGCTAAGGGGCCGGCACAGCCCATAAAAACCTTca                                                                                                                <  8:161028/148‑1 (MQ=255)
                                     gTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTcag                                                                                                               <  6:206193/149‑1 (MQ=255)
                                        tGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTCGTGTAAGCGGTGCTGGCCCCTTCGCGGGTTTACACGCGAAGGGGCCGGCACAGCCCATAAAAAACTTcaccc                                                                                                             >  8:124923/1‑145 (MQ=255)
                                               gcggcgTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGcac                                                                                                                                  >  3:73379/1‑120 (MQ=255)
                                               gcggcgTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGCGGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCa                                                                                                     <  2:63232/149‑1 (MQ=255)
                                                         ccGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCa                                                                                           <  7:133872/149‑1 (MQ=255)
                                                          cGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCAc                                                                                          <  6:266323/149‑1 (MQ=255)
                                                                 gagaCCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGa                                                                                   >  7:153714/1‑149 (MQ=255)
                                                                 gagaCCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGa                                                                                   >  7:149751/1‑149 (MQ=255)
                                                                       aaccaGGTGTCGGGCAAGCTTGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGTTTTACCGGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCATAGTCGGCGACCCATTGATCTCgg                                                                             <  5:201487/149‑1 (MQ=255)
                                                                        accaGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGc                                                                            <  8:153714/149‑1 (MQ=255)
                                                                        accaGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGc                                                                            <  8:149751/149‑1 (MQ=255)
                                                                          caGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCAt                                                                          >  8:71145/1‑149 (MQ=255)
                                                                                 cGGGCAAGCTGGTGATGGTGAGCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCGTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTcgcg                                                                   <  7:10514/149‑1 (MQ=255)
                                                                                  gggCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCgcgc                                                                  <  6:70243/149‑1 (MQ=255)
                                                                                    gCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCaa                                                                >  3:126178/1‑149 (MQ=255)
                                                                                                                     tgtAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATc                               <  3:240089/149‑1 (MQ=255)
                                                                                                                          gCGGGTCTGGCCCCTTCGCGGTTTACCCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCActc                          <  3:155380/149‑1 (MQ=255)
                                                                                                                          gCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCgg                                     <  5:112761/138‑1 (MQ=255)
                                                                                                                          gCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCgg                                     >  6:112761/1‑138 (MQ=255)
                                                                                                                                  ggCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTcaac                     >  4:62460/1‑146 (MQ=255)
                                                                                                                                  ggCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTcaac                     <  3:62460/146‑1 (MQ=255)
                                                                                                                                   gCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCAGTGATCTCGGCATGCTCGCGCAACCCTGCGTCCGGGTTCCCCACATGCGGGTGATCCCCTCTccacaac                  >  3:245209/1‑148 (MQ=255)
                                                                                                                                   gCCCCTTCGCGGGTAAACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTcaacaac                  >  2:100126/1‑148 (MQ=255)
                                                                                                                                     cccTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCgg                                                    <  3:237450/112‑1 (MQ=255)
                                                                                                                                     cccTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCgg                                                    >  4:237450/1‑112 (MQ=255)
                                                                                                                                      ccTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACg              <  7:71145/149‑1 (MQ=255)
                                                                                                                                       cTTCGCGGGTAAACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACt                                                            <  3:67058/102‑1 (MQ=255)
                                                                                                                                       cTTCGCGGGTAAACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACt                                                            >  4:67058/1‑102 (MQ=255)
                                                                                                                                        ttCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACgg             >  4:116366/1‑148 (MQ=255)
                                                                                                                                        ttCGCGGGTAAACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCTGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACgg             >  2:206098/1‑148 (MQ=255)
                                                                                                                                        ttCGCGGGTAAACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTcaa                      <  7:136316/139‑1 (MQ=255)
                                                                                                                                        ttCGCGGGTAAACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTcaa                      >  8:136316/1‑139 (MQ=255)
                                                                                                                                        ttCGCGGGTAAACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACgg             >  2:3674/1‑148 (MQ=255)
                                                                                                                                                  tACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGGCAAGTCATTcc  >  7:101601/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGGCAAGTCATTCC  >  NC_002947/1921404‑1921698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑