Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,933,664 G→T 10.6% R30R (CGC→CGA)  minD ← MinC‑MinD‑MinE system ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,933,6640GT10.6% 123.4 / 3.6 44R30R (CGC→CGA) minDMinC‑MinD‑MinE system ATPase
Reads supporting (aligned to +/- strand):  ref base G (19/20);  new base T (2/3);  total (21/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCTTGATCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAG  >  NC_002947/1933524‑1933809
                                                                                                                                            |                                                                                                                                                 
tCCTTGATCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGc                                                                                                                                           <  2:42638/149‑1 (MQ=255)
tCCTTGATCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCACAGTGc                                                                                                                                           <  2:134566/149‑1 (MQ=255)
  cTTGATCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGa                                                                                                                                         >  8:95530/1‑149 (MQ=255)
   ttGATCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAg                                                                                                                                        >  6:139961/1‑149 (MQ=255)
   ttGATCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGAGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCTCGCACTGCgcg                                                                                                                                        >  7:175577/1‑147 (MQ=255)
     gATCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAg                                                                                                                                        <  1:197830/147‑1 (MQ=255)
      aTCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCTCGCACTGCGCGGcc                                                                                                                                     <  4:62487/149‑1 (MQ=255)
      aTCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCTCGCACTGCGCGGcc                                                                                                                                     <  6:73407/149‑1 (MQ=255)
      aTCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCTCGCACTGCGCGGc                                                                                                                                      <  8:175577/148‑1 (MQ=255)
      aTCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGc                                                                                                                                      <  3:233926/148‑1 (MQ=255)
       tCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCg                                                                                                                                    >  1:246852/1‑149 (MQ=255)
             ccTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGCCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGcc                                                                                                                                     >  7:245549/1‑142 (MQ=255)
             ccTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGCCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGcc                                                                                                                                     <  8:245549/142‑1 (MQ=255)
                 ttGCAGGTTGGCTTCGCCGTGGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATgg                                                                                                                          <  7:172227/149‑1 (MQ=255)
                     aGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAAt                                                                                                                            >  6:211721/1‑143 (MQ=255)
                     aGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAAt                                                                                                                            <  5:211721/143‑1 (MQ=255)
                          ggCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTa                                                                                                                                 <  2:78890/133‑1 (MQ=255)
                          ggCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTa                                                                                                                                 >  1:78890/1‑133 (MQ=255)
                                   gTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCt                                                                                                        >  2:228818/1‑149 (MQ=255)
                                        ccACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGcc                                                                                                    >  5:234820/1‑148 (MQ=255)
                                           cGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCcaca                                                                                                <  5:139961/149‑1 (MQ=255)
                                               gACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACAcccc                                                                                            >  6:177524/1‑149 (MQ=255)
                                                        gTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCgg                                                                                   <  3:264311/149‑1 (MQ=255)
                                                           caccacGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGaa                                                                                 <  2:246852/148‑1 (MQ=255)
                                                              cacGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTaa                                                                             <  1:228818/149‑1 (MQ=255)
                                                                 gcggcgTTCGCAGCCCATGATCAGGTCGAGGTTACGGAGGCCGACGTCGAAGTCGACAATGACTGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCa                                                                          <  1:107724/149‑1 (MQ=255)
                                                                 gcggcgTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCa                                                                          >  5:142191/1‑149 (MQ=255)
                                                                            aGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTg                                                               <  6:50537/149‑1 (MQ=255)
                                                                              cccATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGc                                                             >  4:48145/1‑149 (MQ=255)
                                                                                cATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTg                                                                                                            >  4:158573/1‑100 (MQ=255)
                                                                                cATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTg                                                                                                            <  3:158573/100‑1 (MQ=255)
                                                                                 aTGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCcaca                                                                                                >  5:213718/1‑111 (MQ=255)
                                                                                 aTGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCcaca                                                                                                <  6:213718/111‑1 (MQ=255)
                                                                                         gTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTc                                                  <  3:48145/149‑1 (MQ=255)
                                                                                                   cGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTaaa                                         <  7:95530/148‑1 (MQ=255)
                                                                                                    gCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTaaaaa                                       >  2:225299/1‑149 (MQ=255)
                                                                                                            aCGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCggg                               <  5:177524/149‑1 (MQ=255)
                                                                                                             cGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGAGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGa                              >  7:125441/1‑149 (MQ=255)
                                                                                                                  aaGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACtt                         >  4:119725/1‑149 (MQ=255)
                                                                                                                       gACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTccc                    >  8:97774/1‑149 (MQ=255)
                                                                                                                             gACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCaa               >  5:156213/1‑148 (MQ=255)
                                                                                                                                    taccggCCTCGCACTGCGCGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCACGGTGTTTCACCCCTAAAATTAGTCGGGACACTTGTCCCTGCAAAAAACTg        >  5:156103/5‑148 (MQ=255)
                                                                                                                                      gtgGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGa       <  6:234820/147‑1 (MQ=255)
                                                                                                                                         gCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAg  >  1:270457/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                 
TCCTTGATCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAG  >  NC_002947/1933524‑1933809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑