Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,944,531 T→A 9.1% intergenic (+32/+230) actP‑I → / ← PP_1744 acetate permease/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,944,5310TA 9.1% 153.2 / 6.1 55intergenic (+32/+230)actP‑I/PP_1744acetate permease/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (19/31);  new base A (3/2);  total (22/33)
Fisher's exact test for biased strand distribution p-value = 3.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.41e-01

TTCTTCTCGGTCACCGACAAGTCCAAGGCGGCTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATACTGTTTAATCAC  >  NC_002947/1944386‑1944674
                                                                                                                                                 |                                                                                                                                               
ttcttcTCGGTCACCGACAAGTCCAAGGCGGCTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTc                                                                                                                                              <  7:179094/149‑1 (MQ=255)
  cttctCGGTCTCCGACAAGACCAAGGCGGCTCTCGATCAGCGCGCGCTGTTCTTCCCGATGTTCTTGGGTTGGCAGACTCGCCTGGGTGCCTGCGGGGGGGTCTCGCACTAACTTCAATCCCCTCCATTGAAAACGCCCCGCTTGTCg                                                                                                                                             <  2:264140/148‑1 (MQ=255)
     ctcGGTCACCGACAAGTCCAAGGCGGCTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCggg                                                                                                                                           <  5:164223/147‑1 (MQ=255)
         gTCACCGACAAGTCCAAGGCGGCTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGttt                                                                                                                                     <  6:17093/149‑1 (MQ=255)
          tCACCGACAAGTCCAAGGCGGCTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGtttt                                                                                                                                    >  7:66354/1‑149 (MQ=255)
           cACCGACAAGTCCAAGGCGGCTGAGGATGAGCGCGCGCTGTTCTTGCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGttttt                                                                                                                                   >  1:56009/1‑149 (MQ=255)
            aCCGACAAGTCCAAGGCGGCTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGtttttt                                                                                                                                  <  3:70823/149‑1 (MQ=255)
             ccGACAAGTCCAAGGCGGCTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCTCTAACATCAATCCCCTGCATTGAAAACGCCCCGACAAGCGGGGCGttttcaa                                                                                                                                 <  7:191995/149‑4 (MQ=255)
                 cAAGTCCAAGGCGGCTGAGGATGAGCGCGCGCTGTTCTTCCCGATTTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTc                                                                                                                             <  1:225171/149‑1 (MQ=255)
                   aGTCCAAGGCGGCTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCAt                                                                                                                           <  8:18611/149‑1 (MQ=255)
                        aaGGCGGCTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGttttt                                                                                                                                   <  4:40793/136‑1 (MQ=255)
                        aaGGCGGCTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGttttt                                                                                                                                   >  3:40793/1‑136 (MQ=255)
                                    gATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTAttt                                                                                                                              <  4:209553/129‑1 (MQ=255)
                                    gATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTAttt                                                                                                                              >  3:209553/1‑129 (MQ=255)
                                             gcgcTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGttttt                                                                                                                                   >  6:6078/1‑115 (MQ=255)
                                             gcgcTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGttttt                                                                                                                                   <  5:6078/115‑1 (MQ=255)
                                                        cccGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCGAGACAGGTTGGTGctct                                                                                       <  7:6397/148‑1 (MQ=255)
                                                        cccGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGctct                                                                                       <  2:36478/148‑1 (MQ=255)
                                                         ccGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCTATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGc                                                                                     <  2:56009/149‑1 (MQ=255)
                                                         ccGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGc                                                                                     >  6:178372/1‑149 (MQ=255)
                                                         ccGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGc                                                                                     >  5:246010/1‑149 (MQ=255)
                                                         ccGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGc                                                                                     >  6:201221/1‑149 (MQ=255)
                                                          cGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGcc                                                                                    >  4:110117/1‑149 (MQ=255)
                                                          cGCAGTTCGTGCGTTCGCAGACAGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGACAAGCGGGGCGtttt                                                                                                                                    >  7:168622/1‑101 (MQ=38)
                                                              gTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGTGGCGGTCTCGCACTAACATCAATCCCATGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATg                                                                                <  8:121558/149‑1 (MQ=255)
                                                                   tGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTccc                                                                           <  6:246010/149‑1 (MQ=255)
                                                                         cGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCAt                                                                      <  3:121010/148‑1 (MQ=255)
                                                                            aGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCg                                                                  >  6:79508/1‑149 (MQ=255)
                                                                             gACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTGGGGGCGTTTTTTATTTCATGCAGACGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGt                                                                 <  8:60743/149‑1 (MQ=255)
                                                                             gACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGt                                                                 <  5:178372/149‑1 (MQ=255)
                                                                             gACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGt                                                                 <  8:66354/149‑1 (MQ=255)
                                                                                   ccTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAgcgc                                                           <  3:110117/149‑1 (MQ=255)
                                                                                         tGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCAt                                                     <  1:119764/149‑1 (MQ=255)
                                                                                                 gggCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACg                                             >  6:7656/1‑149 (MQ=255)
                                                                                                   gCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGc                                           <  5:201221/149‑1 (MQ=255)
                                                                                                           gCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGt                                                                 >  6:111238/1‑119 (MQ=255)
                                                                                                           gCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGt                                                                 <  5:111238/119‑1 (MQ=255)
                                                                                                            cACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCg                                                                                                                    >  2:255149/1‑67 (MQ=255)
                                                                                                            cACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCg                                                                                                                    <  1:255149/67‑1 (MQ=255)
                                                                                                                   aTCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGcc                                                          >  5:212190/1‑118 (MQ=255)
                                                                                                                   aTCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGcc                                                          <  6:212190/118‑1 (MQ=255)
                                                                                                                           ccTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAg                   >  4:213500/1‑149 (MQ=255)
                                                                                                                             tGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTc                                                       <  7:83081/111‑1 (MQ=255)
                                                                                                                             tGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGaa                    <  8:234803/146‑1 (MQ=255)
                                                                                                                             tGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGGCAATGAAGc                  >  7:234803/1‑148 (MQ=255)
                                                                                                                             tGCATTGAAAACGCCCCGCTTGTCGCGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCACACAGCTTCGTGCTCTGCCCATGCTCCCCTCATGTCGTCAGCGCCTTc                                                       >  8:83081/1‑111 (MQ=255)
                                                                                                                              aaataaaaaaCGCCCCGACAAGCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCa                >  6:140137/7‑149 (MQ=255)
                                                                                                                                ataaaaaaCGCCCCGACAAGCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATa              <  5:140137/145‑1 (MQ=255)
                                                                                                                                  tGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATACt            >  4:149793/1‑149 (MQ=255)
                                                                                                                                    aaaaCGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATACTGt          <  3:213500/149‑1 (MQ=255)
                                                                                                                                    aaaaCGCCCCGACAAGCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATACTGt          >  7:186814/1‑149 (MQ=255)
                                                                                                                                     aaaCGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATACTGtt         >  2:100739/1‑149 (MQ=255)
                                                                                                                                        cGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATACTGTTTaa      <  4:36968/149‑1 (MQ=255)
                                                                                                                                          ccccGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATACTGTTTAATc    <  1:100739/149‑1 (MQ=255)
                                                                                                                                            ccGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATACTGTTTAATCAc  <  5:7656/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TTCTTCTCGGTCACCGACAAGTCCAAGGCGGCTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATACTGTTTAATCAC  >  NC_002947/1944386‑1944674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑