Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,953,927 C→T 11.4% A84V (GCC→GTC)  PP_1752 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,953,9270CT11.4% 86.7 / 3.0 35A84V (GCC→GTC) PP_1752hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (14/17);  new base T (2/2);  total (16/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.19e-01

AAGCAGCAGGCGCGTTTTCCGGTGATCGTGCTGATCAACGGCATTGAAGGCGCCGGCAAGGGTGAGACGGTAAAACTGCTCAACGAGTGGATGGACCCGCGCATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTCTTTGGCAACTGGTACAGCCAGATGCTGCAGGGGCGGGTGCACGGGGTGTTCAAGGATGCCGTGCTCGATCAGGCCATTACCGGTG  >  NC_002947/1953779‑1954073
                                                                                                                                                    |                                                                                                                                                  
aaGCAGCAGGCGCGTTTTCCGGTGATCGTGCTGATCAACGGCATTGAAGGCGCCGGCAAGGGTGAGACGGTAAAACTGCTCAACGAGTGGATGGACCCGCGCATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGgc                                                                                                                                                    >  8:38241/1‑149 (MQ=255)
aaGCAGCAGGCGCGTTTTCCGGTGATCGTGCTGATCAACGGCATTGAAGGCGCCGGCAAGGGTGAGACGGTAAAACTGCTCAACGAGTGGATGGACCCGCGCATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGgc                                                                                                                                                    >  6:235320/1‑149 (MQ=255)
aaGCAGCAGGCGCGTTTTCCGGTGATCGTGCTGATCAACGGCATTGAAGGCGCCGGCAAGGGTGAGACGGTAAAACTGCTCAACGAGTGGATGGACCCGCGCATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGgc                                                                                                                                                    >  7:226824/1‑149 (MQ=255)
aaGCAGCAGGCGCGTTTTCCGGTGATCGTGCTGATCAACGGCATTGAAGGCGCCGGCAAGGGTGAGACGGTAAAACTGCTCAACGAGTGGATGGACCCGCGCATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGgc                                                                                                                                                    >  3:176134/1‑149 (MQ=255)
         gcgcGTTTTCCGTTGATCGTGCTGATCAACGGCATTGAAGGCGCCGGCAAGGGTGAGACGGTAAAACTGCTCAACGAGTGGATGGACCCGCGCATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGgcccgcccgcc                                                                                                                                           <  4:176134/149‑1 (MQ=255)
             gTTTTCCGGTGATCGTGCTGATCAACGGCATTGAAGGCGCCGGCAAGGGTGAGACGGTAAAACTGCTCAACGAGTGGATGGACCCGCGCATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGGCCcgcccgcccgcc                                                                                                                                       >  7:159815/1‑149 (MQ=255)
                ttCCGGTGATCGTGCTGATCAACGGCATTGAAGGCGCCGGCAAGGGTGAGACGGTAAAACTGCTCAACGAGTGGATGGACCCGCGCATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGGCCCGCCCGCCCGCCTgg                                                                                                                                    >  8:5363/1‑149 (MQ=255)
                  ccGGTGATCGTGCTGATCAACGGCATTGAAGGCGCCGGCAAGGGTGAGACGGTAAAACTGCTCAACGAGTGGATGGACCCGCGCATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGGCCCGCCCGCCCGCCTGgc                                                                                                                                   <  7:38241/148‑1 (MQ=255)
                                  tCAACGGCATTGAAGGCGCCGGCAAGGGTGAGACGGTAAAACTGCTCAACGAGTGGATGGACCCGCGCATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTg                                                                                                                  <  8:159815/149‑1 (MQ=255)
                                         cATTGAAGGCGCCGGCAAGGGTGAGACGGTAAAACTGCTCAACGAGTGGATGGACCCGCGCATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGGCCTGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGccc                                                                                                            >  4:218907/1‑148 (MQ=255)
                                           ttGAAGGCGCCGGCAAGGGTGAGACGGTAAAACTGCTCAACGAGTGGATGGACCCGCGCATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAg                                                                                                         <  7:191853/149‑1 (MQ=255)
                                                ggCGCCGGCAAGGGTGAGACGGTAAAACTGCTCAACGAGTGGATGGACCCGCGCATGATCGATGTGCTCACCTTCGCCCAGCAGACCGACGAAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGc                                                                                                     >  2:221970/1‑148 (MQ=255)
                                                             gTGAGACGGTAAAACTGCTCAACGAGTGGATGGACCCGCGCATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGGCCTGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTc                                                                                       <  3:218907/149‑1 (MQ=255)
                                                                  aCGGTAAAACTTCTCAAAGAGTGGATGGACCCGCTCATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCgg                                                                                                                        <  2:16742/111‑1 (MQ=255)
                                                                  aCGGTAAAACTGCTCAACGAGTGGATGGACCCGCGCATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTCTTTgg                                                                                  <  5:235320/149‑1 (MQ=255)
                                                                      tAAAACTGCTCAACGAGTGGATGGACCCGCGCATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTCTTTGGCAAc                                                                              >  3:213130/1‑149 (MQ=255)
                                                                        aaaCTGCTCAACGAGTGGATGGACCCGCGCATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTCTTTGGCAACt                                                                             <  4:213130/148‑1 (MQ=255)
                                                                              cTCAACGATTGGATGGACCCGCGCATGATCGATGTGCTCACCTTCGACCATCAGACCGACGAAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCTAATGGGCGTTTTCTTTGGCAACTGGTACa                                                                       <  2:49339/148‑1 (MQ=255)
                                                                                  aCGAGTGGATGGACCCGCGCATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTCTTTGGCAACTGGAACAGc                                                                     >  5:84446/1‑146 (MQ=255)
                                                                                          atggaCCCGCGCATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGGTCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTCTTTGGCAACTGGTACAGCCAGAtgctg                                                            >  2:58816/1‑147 (MQ=255)
                                                                                          atggaCCCGCGCATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGGTCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTCTTTGGCAACTGGTACAGCCAGAtgctg                                                            >  6:222277/1‑147 (MQ=255)
                                                                                          atggaCCCGCGCATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTCTTTGGCAACTGGTACAGCCAGAtgctg                                                            >  8:136482/1‑147 (MQ=255)
                                                                                           tggaCCCGCGCATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTCTTTGGCAACTGGTACAGCCAGATGCTGCAg                                                         <  7:136482/149‑1 (MQ=255)
                                                                                                ccGCGCATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTCTTTGGCAACTGGTACAGCCAGATGCTGCAGGGGCg                                                    >  7:103840/1‑149 (MQ=255)
                                                                                                ccGCGCATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGGTTTCTTTGGCAACTGGTACAGCCAGATGCTGCAGGGGCg                                                    >  2:244136/1‑149 (MQ=255)
                                                                                                     cATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGGTCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTCTTTGGCAACTGGTACAGCCAGATGCTGCAGGGGCGGGTGc                                               <  5:222277/149‑1 (MQ=255)
                                                                                                     cATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGGTCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTCTTTGGCAACTGGTACAGCCAGATGCTGCAGGGGCGGGTGc                                               <  1:58816/149‑1 (MQ=255)
                                                                                                         aTCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTCTTTGGCAACTGGTACAGCCAGATGCTGCAGGGGCgg                                                   >  8:247990/1‑141 (MQ=255)
                                                                                                         aTCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTCTTTGGCAACTGGTACAGCCAGATGCTGCAGGGGCgg                                                   <  7:247990/141‑1 (MQ=255)
                                                                                                            gATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTCTTTGGCAACTGGTACAGCCAGATGCTGCAGGGGCGGGTGCACggg                                          <  6:226929/147‑1 (MQ=255)
                                                                                                            gATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTCTTTGGCAACTGGTACAGCCAGATGCTGCAGGGGCGGGTGCACggg                                          >  5:226929/1‑147 (MQ=255)
                                                                                                                  cTCACCTTCGACCAGCAGACCGACGAAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTCTTTGGCAACTGGTACAGCCAGATGCTGCAGGGGCGGGTGCACGGGGTGTTCaa                                  <  1:244136/149‑1 (MQ=255)
                                                                                                                  cTCACCTTCGACCAGCAGACCGACGAAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTCTTTGGCAACTGGTACAGCCAGATGCTGCAGGGGCGGGTGCACGGGGTGTTCaa                                  <  7:5363/149‑1 (MQ=255)
                                                                                                                             ccAGCAGACCGACGAAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTCTTTGGCAACTGGTACAGCCAGATGCTGCAGGGGCGGGTGCACGGGGTGTTCaa                                  <  6:274594/138‑1 (MQ=255)
                                                                                                                             ccAGCAGACAGACGAAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTCTTTGGCAACTGGTACAGCCAGATGCTGCAGGGGCGGGTGCACGGGGTGTTCaa                                  >  5:274594/1‑138 (MQ=255)
                                                                                                                               agcagACCGACGAAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTCTTTGGCAACTGGTACAGCCAGATGCTGCAGGGGCGGGTGCACGGGGTGTTCAAGGATGCCGTGCTc                     <  6:84446/149‑1 (MQ=255)
                                                                                                                                      cgacgaAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTCTTTGGCAACTGGTACAGCCAGATGCTGCAGGGGCGGGTGCACGGGG‑GTTCAAGGATGCCGTGCGCGATCAGGc             >  1:53422/1‑149 (MQ=255)
                                                                                                                                         cgaAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTCTTTGGCAACTGGTACAGCCAGATGCTGCAGGGGCGGGTGCACgg                                           >  2:7643/1‑117 (MQ=255)
                                                                                                                                         cgaAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTCTTTGGCAACTGGTACAGCCAGATGCTGCAGGGGCGGGTGCACgg                                           <  1:7643/117‑1 (MQ=255)
                                                                                                                                                 tGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTCTTTGGCAACTGGTACAGCCAGATGCTGCAGGGGCGGGTGCAC‑GGGTGTTCAAGGATGCCGTGCTCGATCAGGCCATTACCGGTg  <  2:53422/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AAGCAGCAGGCGCGTTTTCCGGTGATCGTGCTGATCAACGGCATTGAAGGCGCCGGCAAGGGTGAGACGGTAAAACTGCTCAACGAGTGGATGGACCCGCGCATGATCGATGTGCTCACCTTCGACCAGCAGACCGACGAAGAGCTGGCCCGCCCGCCCGCCTGGCGCTATTGGCGGGCCTTGCCGCCCAAGGGGCGAATGGGCGTTTTCTTTGGCAACTGGTACAGCCAGATGCTGCAGGGGCGGGTGCACGGGGTGTTCAAGGATGCCGTGCTCGATCAGGCCATTACCGGTG  >  NC_002947/1953779‑1954073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑