Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,989,572	T→C	7.7%	G274G (GGT→GGC) ‡	PP_1780 →	mannosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,989,572	0	T	C	7.7%	184.0 / 4.5	67	G274G (GGT→GGC) ‡	PP_1780	mannosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (29/31);  new base C (3/2);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

CAGCGCCTTCGATGCTGAGTATGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGCGTATGGCGG  >  NC_002947/1989428‑1989720                                                                                                                                                 |                                                                                                                                                     cAGCGCCTTCGATGCTGAGTATGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGc                                                                                                                                                  <  5:277423/149‑1 (MQ=255)       cTTCGATGCTGAGTATGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAgg                                                                                                                                            <  6:30084/149‑1 (MQ=255)        ttCGATGCTGAGTATGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAgg                                                                                                                                            >  4:121803/1‑148 (MQ=255)            aTGCTGAGTATGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGcc                                                                                                                                       >  7:133421/1‑149 (MQ=255)               cTGAGTATGGGGTGACCCTTGAGGACCTGGCGGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGTGCTGACCCAGCTCCAggttaactt                                                                                                                                     <  3:194160/148‑8 (MQ=255)                 gAGTATGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTtgt                                                                                                                                  >  8:214716/1‑149 (MQ=255)                   gTATGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTtgtg                                                                                                                                 >  6:268260/1‑148 (MQ=255)                    tATGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTgg                                                                                                                                <  2:135512/148‑1 (MQ=255)                      tGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGAtt                                                                                                                             >  3:217114/1‑149 (MQ=255)                        gggTGACCCTTGAGGACCTGGCTGAGCGCTATAAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGGCAGCACCAGGCAGCCCTTGTGGAttt                                                                                                                            <  4:66911/148‑1 (MQ=255)                         ggTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGAttt                                                                                                                            >  4:137788/1‑147 (MQ=255)                           tGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGc                                                                                                                        <  8:99797/149‑1 (MQ=255)                              cccTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAg                                                                                                                     >  2:209644/1‑149 (MQ=255)                               ccTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCACAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAgg                                                                                                                    <  1:209644/149‑1 (MQ=255)                               acTTGAGGACCTGGATCAGCGCTATGAAGAAACGCTGGCTAGCAACTTCCAGCAAGTGCGCGAAAGCGTCGATAGATGCCATACTGCATTGACGCAAGATAACCAGGACATGCGACAGCAACACGCAGACCTTGTGGATGAGAGCCAg                                                                                                                     >  8:222240/2‑148 (MQ=255)                                   gAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGt                                                                                                                >  4:12300/1‑149 (MQ=255)                                    aGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTc                                                                                                               <  3:154095/149‑1 (MQ=255)                                      gACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGa                                                                                                             <  8:133421/149‑1 (MQ=255)                                       aCCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAg                                                                                                            <  5:202986/149‑1 (MQ=255)                                        ccTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGc                                                                                                           <  3:12300/149‑1 (MQ=255)                                           ggCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCAAGCAAGTGGGCGAAAGCGTCGAAATCTGCAATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGAACCAGGCAGCACTTGTGGATTTGAGCCAGTAAGTAGAGCGt                                                                                                         >  3:210945/1‑148 (MQ=255)                                            gCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGt                                                                                                         <  8:214663/147‑1 (MQ=255)                                            gCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCa                                                                                                       >  3:93613/1‑149 (MQ=255)                                              tGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATc                                                                                                     <  1:167145/149‑1 (MQ=255)                                                    cTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCg                                                                                               <  7:214716/149‑1 (MQ=255)                                                    cTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATGCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTTTGGATTTGAGCCAAGAAGTCGAGCGAAATAGCGcc                                                                                                >  8:190125/1‑148 (MQ=255)                                                     tATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCCTTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCg                                                                                               >  6:268353/1‑148 (MQ=255)                                                     tATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCg                                                                                               >  5:201043/1‑148 (MQ=255)                                                       tGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAAc                                                                                            >  3:20847/1‑149 (MQ=255)                                                          agaaAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTgg                                                                                         <  4:217114/149‑1 (MQ=255)                                                           gaaAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTg                                                                                          >  5:273955/1‑147 (MQ=255)                                                             aaGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGgaga                                                                                      <  7:181833/149‑1 (MQ=255)                                                               ggctggctAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGc                                                                                    <  3:121803/149‑1 (MQ=255)                                                                  tggctAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCt                                                                                  >  6:21842/1‑148 (MQ=255)                                                                   ggctAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTc                                                                                 >  3:196710/1‑148 (MQ=255)                                                                      taccAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGa                                                                                                                          >  7:225838/4‑104 (MQ=255)                                                                      tAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCGTTGTGGATTTGa                                                                                                                          <  8:225838/104‑1 (MQ=255)                                                                      tAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTcaa                                                                               >  5:265155/1‑147 (MQ=255)                                                                      tAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTcaa                                                                               <  6:265155/147‑1 (MQ=255)                                                                       aGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTcaagc                                                                             <  4:210945/148‑1 (MQ=255)                                                                          aaCTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAgt                                                                         >  8:32185/1‑149 (MQ=255)                                                                           aCTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAgtg                                                                        <  6:273955/149‑1 (MQ=255)                                                                                 aGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAAc                                                                  >  8:136663/1‑149 (MQ=255)                                                                                 aGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGCCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAAc                                                                  >  2:161547/1‑149 (MQ=255)                                                                                     aGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGcc                                                              <  5:258129/149‑1 (MQ=255)                                                                                          gCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAAGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAac                                                         <  7:190125/149‑1 (MQ=255)                                                                                           cGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCGAGTGGTGAACGGCCTGAac                                                         >  5:139385/1‑148 (MQ=255)                                                                                           cGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAacc                                                        <  4:93613/149‑1 (MQ=255)                                                                                              aaGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGGGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAaccacc                                                     <  5:268260/149‑1 (MQ=255)                                                                                                     gAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGt                                               <  4:196710/148‑1 (MQ=255)                                                                                                     gAAAGCTGCAATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGt                                               <  7:136663/148‑1 (MQ=255)                                                                                                             ccATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGa                                           >  4:247204/1‑144 (MQ=255)                                                                                                             ccATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGa                                           <  3:247204/144‑1 (MQ=255)                                                                                                             ccATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATAcc                                       >  3:187303/1‑148 (MQ=255)                                                                                                                     cATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGcc                                                              <  8:252213/117‑1 (MQ=255)                                                                                                                          tCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCATGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCTTCTGTCCGATACCCAGCAACggctgg                          <  5:153834/148‑1 (MQ=255)                                                                                                                                    aCCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCtgat               <  4:187303/149‑1 (MQ=255)                                                                                                                                    aCCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCtgat               >  4:169250/1‑149 (MQ=255)                                                                                                                                     ccTGGTGCTGACCCAGCTCCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAAc                                <  3:226/131‑1 (MQ=255)                                                                                                                                     ccTGGTGCTGACCCAGCTCCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAAc                                >  4:226/1‑131 (MQ=255)                                                                                                                                     ccTGGTGCTGACCCAGCTCCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCtgat               >  5:140172/1‑148 (MQ=255)                                                                                                                                     ccTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGACTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCtgat               >  6:39787/1‑148 (MQ=255)                                                                                                                                         gAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGc           <  5:21842/148‑1 (MQ=255)                                                                                                                                           gCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAac                                                         >  3:86880/1‑100 (MQ=255)                                                                                                                                           gCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAac                                                         <  4:86880/100‑1 (MQ=255)                                                                                                                                             tGGGTCAGCACCAGGCAGCCATTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTCGAGAGCCTCAAGCAAGTGGTGAACGACCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGATGATGAGCGTATg      >  7:122627/1‑149 (MQ=255)                                                                                                                                               ggTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGCGTATggc    >  2:76247/1‑149 (MQ=255)                                                                                                                                                 tCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGCGTATggcgg  <  7:32185/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                     CAGCGCCTTCGATGCTGAGTATGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGCGTATGGCGG  >  NC_002947/1989428‑1989720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑