Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,989,577	A→T	5.9%	H276L (CAC→CTC)	PP_1780 →	mannosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,989,577	0	A	T	5.9%	199.7 / 2.9	68	H276L (CAC→CTC)	PP_1780	mannosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (32/32);  new base T (2/2);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.15e-01

CTTCGATGCTGAGTATGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGCGTATGGCGGCGAC  >  NC_002947/1989434‑1989724                                                                                                                                                |                                                                                                                                                    cTTCGATGCTGAGTATGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAgg                                                                                                                                                <  6:30084/149‑1 (MQ=255)  ttCGATGCTGAGTATGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAgg                                                                                                                                                >  4:121803/1‑148 (MQ=255)      aTGCTGAGTATGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGcc                                                                                                                                           >  7:133421/1‑149 (MQ=255)         cTGAGTATGGGGTGACCCTTGAGGACCTGGCGGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGTGCTGACCCAGCTCCAggttaactt                                                                                                                                         <  3:194160/148‑8 (MQ=255)           gAGTATGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTtgt                                                                                                                                      >  8:214716/1‑149 (MQ=255)             gTATGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTtgtg                                                                                                                                     >  6:268260/1‑148 (MQ=255)              tATGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTgg                                                                                                                                    <  2:135512/148‑1 (MQ=255)                tGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGAtt                                                                                                                                 >  3:217114/1‑149 (MQ=255)                  gggTGACCCTTGAGGACCTGGCTGAGCGCTATAAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGGCAGCACCAGGCAGCCCTTGTGGAttt                                                                                                                                <  4:66911/148‑1 (MQ=255)                   ggTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGAttt                                                                                                                                >  4:137788/1‑147 (MQ=255)                     tGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGc                                                                                                                            <  8:99797/149‑1 (MQ=255)                        cccTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAg                                                                                                                         >  2:209644/1‑149 (MQ=255)                         ccTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCACAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAgg                                                                                                                        <  1:209644/149‑1 (MQ=255)                         acTTGAGGACCTGGATCAGCGCTATGAAGAAACGCTGGCTAGCAACTTCCAGCAAGTGCGCGAAAGCGTCGATAGATGCCATACTGCATTGACGCAAGATAACCAGGACATGCGACAGCAACACGCAGACCTTGTGGATGAGAGCCAg                                                                                                                         >  8:222240/2‑148 (MQ=255)                             gAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGt                                                                                                                    >  4:12300/1‑149 (MQ=255)                              aGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTc                                                                                                                   <  3:154095/149‑1 (MQ=255)                                gACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGa                                                                                                                 <  8:133421/149‑1 (MQ=255)                                 aCCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAg                                                                                                                <  5:202986/149‑1 (MQ=255)                                  ccTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGc                                                                                                               <  3:12300/149‑1 (MQ=255)                                     ggCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCAAGCAAGTGGGCGAAAGCGTCGAAATCTGCAATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGAACCAGGCAGCACTTGTGGATTTGAGCCAGTAAGTAGAGCGt                                                                                                             >  3:210945/1‑148 (MQ=255)                                      gCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGt                                                                                                             <  8:214663/147‑1 (MQ=255)                                      gCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCa                                                                                                           >  3:93613/1‑149 (MQ=255)                                        tGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATc                                                                                                         <  1:167145/149‑1 (MQ=255)                                              cTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCg                                                                                                   <  7:214716/149‑1 (MQ=255)                                              cTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATGCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTTTGGATTTGAGCCAAGAAGTCGAGCGAAATAGCGcc                                                                                                    >  8:190125/1‑148 (MQ=255)                                               tATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCCTTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCg                                                                                                   >  6:268353/1‑148 (MQ=255)                                               tATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCg                                                                                                   >  5:201043/1‑148 (MQ=255)                                                 tGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAAc                                                                                                >  3:20847/1‑149 (MQ=255)                                                    agaaAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTgg                                                                                             <  4:217114/149‑1 (MQ=255)                                                     gaaAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTg                                                                                              >  5:273955/1‑147 (MQ=255)                                                       aaGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGgaga                                                                                          <  7:181833/149‑1 (MQ=255)                                                         ggctggctAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGc                                                                                        <  3:121803/149‑1 (MQ=255)                                                            tggctAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCt                                                                                      >  6:21842/1‑148 (MQ=255)                                                             ggctAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTc                                                                                     >  3:196710/1‑148 (MQ=255)                                                                taccAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGa                                                                                                                              >  7:225838/4‑104 (MQ=255)                                                                tAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCGTTGTGGATTTGa                                                                                                                              <  8:225838/104‑1 (MQ=255)                                                                tAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTcaa                                                                                   >  5:265155/1‑147 (MQ=255)                                                                tAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTcaa                                                                                   <  6:265155/147‑1 (MQ=255)                                                                 aGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTcaagc                                                                                 <  4:210945/148‑1 (MQ=255)                                                                    aaCTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAgt                                                                             >  8:32185/1‑149 (MQ=255)                                                                     aCTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAgtg                                                                            <  6:273955/149‑1 (MQ=255)                                                                           aGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAAc                                                                      >  8:136663/1‑149 (MQ=255)                                                                           aGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGCCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAAc                                                                      >  2:161547/1‑149 (MQ=255)                                                                               aGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGcc                                                                  <  5:258129/149‑1 (MQ=255)                                                                                    gCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAAGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAac                                                             <  7:190125/149‑1 (MQ=255)                                                                                     cGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCGAGTGGTGAACGGCCTGAac                                                             >  5:139385/1‑148 (MQ=255)                                                                                     cGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAacc                                                            <  4:93613/149‑1 (MQ=255)                                                                                        aaGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGGGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAaccacc                                                         <  5:268260/149‑1 (MQ=255)                                                                                               gAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGt                                                   <  4:196710/148‑1 (MQ=255)                                                                                               gAAAGCTGCAATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGt                                                   <  7:136663/148‑1 (MQ=255)                                                                                                       ccATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGa                                               >  4:247204/1‑144 (MQ=255)                                                                                                       ccATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGa                                               <  3:247204/144‑1 (MQ=255)                                                                                                       ccATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATAcc                                           >  3:187303/1‑148 (MQ=255)                                                                                                               cATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGcc                                                                  <  8:252213/117‑1 (MQ=255)                                                                                                                    tCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCATGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCTTCTGTCCGATACCCAGCAACggctgg                              <  5:153834/148‑1 (MQ=255)                                                                                                                              aCCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCtgat                   <  4:187303/149‑1 (MQ=255)                                                                                                                              aCCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCtgat                   >  4:169250/1‑149 (MQ=255)                                                                                                                               ccTGGTGCTGACCCAGCTCCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAAc                                    <  3:226/131‑1 (MQ=255)                                                                                                                               ccTGGTGCTGACCCAGCTCCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAAc                                    >  4:226/1‑131 (MQ=255)                                                                                                                               ccTGGTGCTGACCCAGCTCCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCtgat                   >  5:140172/1‑148 (MQ=255)                                                                                                                               ccTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGACTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCtgat                   >  6:39787/1‑148 (MQ=255)                                                                                                                                   gAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGc               <  5:21842/148‑1 (MQ=255)                                                                                                                                     gCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAac                                                             >  3:86880/1‑100 (MQ=255)                                                                                                                                     gCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAac                                                             <  4:86880/100‑1 (MQ=255)                                                                                                                                       tGGGTCAGCACCAGGCAGCCATTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTCGAGAGCCTCAAGCAAGTGGTGAACGACCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGATGATGAGCGTATg          >  7:122627/1‑149 (MQ=255)                                                                                                                                         ggTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGCGTATggc        >  2:76247/1‑149 (MQ=255)                                                                                                                                           tCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGCGTATggcgg      <  7:32185/149‑1 (MQ=255)                                                                                                                                               cACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGCGTATGGCGGCGAc  >  4:56685/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                                    CTTCGATGCTGAGTATGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCCTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGCGTATGGCGGCGAC  >  NC_002947/1989434‑1989724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑