Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,989,797	C→T	8.0%	A349A (GCC→GCT)	PP_1780 →	mannosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,989,797	0	C	T	8.0%	139.4 / 2.4	50	A349A (GCC→GCT)	PP_1780	mannosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (21/24);  new base T (2/2);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.95e-01

TGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGCGTATGGCGGCGACGCAAGCCTGGATCGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTTGCTGCGCAAATTCCCCAAGCTGCACCATCAGTTGAAACTGTTCGCTCAGAACCGCGGGCTTATTGGTGGCGGTGCGTTCGCATCGGCG  >  NC_002947/1989652‑1989944                                                                                                                                                   |                                                                                                                                                    tgAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGCGTATGGCGGCGACGCAAGCCTGGATCGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATc                                                                                                                                                  <  1:248603/149‑1 (MQ=255)   aaCGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGCGTATGGCGGCGACGCAAGCCTGGATCGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCa                                                                                                                                                 <  6:139385/148‑1 (MQ=255)   aaCGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGCGTATGGCGGCGACGCAAGCCTGGATCGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCa                                                                                                                                                 <  4:234407/148‑1 (MQ=255)     cGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGCGTATGGCGGCGACGCAAGCCTGGATCGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGga                                                                                                                                              <  8:155623/149‑1 (MQ=255)      ggCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGCGTATGGCGGCGACGCAAGCCTGGATCGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTCAAAACGCACAGCCAGGCCGCTTGCCATCAGga                                                                                                                                              <  1:161547/149‑1 (MQ=255)                accaTCTGTCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGCGTATGGCGGCGACGCAAGCCTGGATCGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGga                                                                                                                                              >  6:175419/1‑138 (MQ=255)                accaTCTGTCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGCGTATGGCGGCGACGCAAGCCTGGATCGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGga                                                                                                                                              <  5:175419/138‑1 (MQ=255)                        tCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGCGTATGGCGGCGACGCAAGCCTGGATCGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCgg                                                                                                                           >  5:106673/1‑149 (MQ=255)                               cccAGCAACGGCTGGCTCTTGCTGATGAGCGTATGGCGGCGACGCAAGCCTGGATCGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCa                                                                                                                     <  7:153877/148‑1 (MQ=255)                                ccAGCAACGGCTGGCTCTTGCTGATGAGCGTATGGCGGCGACGCAAGCCTGGATCGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAgg                                                                                                                   >  2:116761/1‑149 (MQ=255)                                 cAGCAACGGCTGGCTCTTGCTGATGAGCGTATGGCGGCGACGCAAGCCTGGATCGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGt                                                                                                                  <  1:116761/149‑1 (MQ=255)                                 cAGCAACGGCTGGCTCTTGCTCATGAGCGTATGGCGGCGACGCAACCCTGGATCGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCACGCCGCATCCCATCAGCAAGATACGGTACGTACTCGGCAACCACGt                                                                                                                  >  8:191875/1‑149 (MQ=255)                                    cAACGGCTGGCTCTTGCTGATGAGCGTATGGCGGCGACGCAAGCCTGGATCGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATtcggtcg                                                                                                                                   >  4:88802/1‑129 (MQ=255)                                    cAACGGCTGGCTCTTGCTGATGAGCGTATGGCGGCGACGCAAGCCTGGATCGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATtcggtcg                                                                                                                                   <  3:88802/129‑1 (MQ=255)                                      aCGGCTGGCTCTTGCTGATGAGCGTATGGCGGCGACGCAAGCCTGGATCGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGtgctgc                                                                                                             <  3:169250/149‑1 (MQ=255)                                                 ttGCTGATGAGCGTATGGCGGCGACGCAAGCCTGGATCGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGa                                                                                                           <  3:239237/140‑1 (MQ=255)                                                 ttGCTGATGAGCGTATGGCGGCGACGCAAGCCTGGATCGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGa                                                                                                           >  4:239237/1‑140 (MQ=255)                                                     tgatgaGCGTATGGCGGCGACGCAAGCCTGGATCGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAAc                                                                                               >  7:105325/1‑148 (MQ=255)                                                              tATGGCGGCGACGCAAGCCTGGATCGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGGC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTtgctgc                                                                                     <  6:45437/149‑1 (MQ=255)                                                                tGGCGGCGACGCAAGCCTGGATCGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTTGCTgcgc                                                                                   <  6:106673/149‑1 (MQ=255)                                                                          gCAAGCCTGGATCGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATtcggtcg                                                                                                                                   >  4:190567/1‑91 (MQ=255)                                                                          gCAAGCCTGGATCGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATtcggtcg                                                                                                                                   <  3:190567/91‑1 (MQ=255)                                                                          gCAAGCCTGGATCGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTTGCTGCGCAAATTcccc                                                                          >  8:157813/1‑148 (MQ=255)                                                                             aggcTGGATCGATTAGCAACATGACAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACGAGGTGCTGCGACACAATGCCAACCAGTTGCTGCGGAAATTCCCCAAGc                                                                      <  7:191875/146‑1 (MQ=255)                                                                                 tGGGTCGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTTGCTGCGCAAATTCCCCAAGCTGCa                                                                  >  5:150236/1‑149 (MQ=255)                                                                                      cGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACaa                                                                                                      >  5:68642/1‑108 (MQ=255)                                                                                      cGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACaa                                                                                                      <  6:68642/108‑1 (MQ=255)                                                                                           aGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACATCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCTGGTGCTGCGACACAATGCCAACCAGTTGCTGCGCAAATTCCCCAAGCTGCACCATCAGtt                                                         <  5:236152/148‑1 (MQ=255)                                                                                            gCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCTATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTTGCTGCGCAAATTCCCCAAGCTGCACCATCAGTTg                                                        >  1:102130/1‑148 (MQ=255)                                                                                            gCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTtg                                                                                         >  2:21205/1‑115 (MQ=255)                                                                                            gCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTtg                                                                                         <  1:21205/115‑1 (MQ=255)                                                                                             cAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCTATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTTGCTGCGCAAATTCCCCAAGCTGCAc                                                                 >  3:6849/1‑138 (MQ=255)                                                                                             cAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCTATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTTGCTGCGCAAATTCCCCAAGCTGCAc                                                                 <  4:6849/138‑1 (MQ=255)                                                                                              aaCAGGCGAATTCGCTGGTTGCTTTATGC‑AATCGCACAGCCAGTCCTCTTGCAATAAGGATTATTCGTTCGGTACTCGGCAACCAGTTGGTTCGACAAAAt                                                                                                     <  2:233295/101‑1 (MQ=255)                                                                                              aaCAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTTGCTGCGCAAATTCCCCAAGCTGCACCATCAGTTGaaa                                                     <  7:157813/149‑1 (MQ=255)                                                                                               aCAGGCCAAATCGCTGGTAGCTGTCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTtgctgc                                                                                     >  7:169343/1‑116 (MQ=255)                                                                                                     cAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTTGCTGCGCAAATTCCCCAAGCTGCACCATCAGTTGAAACTGTTCg                                              >  2:11069/1‑149 (MQ=255)                                                                                                       aaTCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTTGCTGCGCAAATTCCCCAAGCTGCACCATCAGTTGAAACTGTTCGCt                                            <  2:39049/149‑1 (MQ=255)                                                                                                                  gCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCTATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTTGCTGCGCAAATTCCCCAAGCTGCACCATCAGTTGAAACTGTTCGCTCAGAACCGCgg                                 <  2:102130/149‑1 (MQ=255)                                                                                                                    tATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTTGCTGCGCAAATTCCCCAAGCTGCACCATCAGTTGAAACTGTTCGCTCAGAACCGCGGGc                               >  7:201569/1‑149 (MQ=255)                                                                                                                          c‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGAATCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTTGCTGCGCAAATTCCCCAAGCTGCACCATCAGTTGAAACTGTTCGCTCAGAACCGCGGGCTTATtg                         >  1:177078/1‑149 (MQ=255)                                                                                                                                 cacaGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTTGCTGCGCAAATTCCCCAAGCTGCACCATCAGTTGAAACTGTTCGCTCAGAACCGCGGGCTTATTGGTGGCg                   <  6:150236/149‑1 (MQ=255)                                                                                                                                   caGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTTGCTGCGCAAATTCCCCAAGCTGCACCATCAGTTGAAACTGTTCGCTCAGAACCGCGGGCTTATTGGTGGCGGt                 <  8:105325/149‑1 (MQ=255)                                                                                                                                    aGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTTGCTGCGCAAATTCCCCAAGCTGCACCATCAGTTGaaa                                                     >  7:207060/1‑112 (MQ=255)                                                                                                                                    aGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTTGCTGCGCAAATTCCCCAAGCTGCACCATCAGTTGaaa                                                     <  8:207060/112‑1 (MQ=255)                                                                                                                                      ccAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTTGCTGCGCAAATTCCCCAAGCTGCACCATCAGTTGAAACTGTTCGCTCAGAACCGCGGGCTTATTGGTGGCGGTGCg              >  4:176699/1‑149 (MQ=255)                                                                                                                                        aGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTTGCTGCGCAAATTCCCCAAGCTGCACCATCAGTTGAAACTGTTCGCTCAGAACCGCGGGCTTATtggt                       >  6:92367/1‑138 (MQ=255)                                                                                                                                        aGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTTGCTGCGCAAATTCCCCAAGCTGCACCATCAGTTGAAACTGTTCGCTCAGAACCGCGGGCTTATtggt                       <  5:92367/138‑1 (MQ=255)                                                                                                                                         ggCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTTGCTGCGCAAATTCCCCAAGCTGCACCATCAGTTGAAACTGTTCGCTCAGAACCGCGGGCTTATTGGTGGCGGTGCGTTc           >  6:5058/1‑149 (MQ=255)                                                                                                                                               ttGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTTGCTGCGCAAATTCCCCAAGCTGCACCATCAGTTGAAACTGTTCGCTCAGAACCGCGGGCTTATTGGTGGCGGTGCGTTCGCATcg     >  1:170374/1‑149 (MQ=255)                                                                                                                                                  ccATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTTGCTGCGCAAATTCCCCAAGCTGCACCATCAGTTGAAACTGTTCGCTCAGAACCGCGGGCTTATTGGTGGCGGTGCGTTCGCATcggcg  <  2:177078/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    TGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGCGTATGGCGGCGACGCAAGCCTGGATCGATAAGCAACAGGCCAAATCGCTGGTAGCTATCGTC‑AAACGCACAGCCAGGCCGCTTGCCATCAGGATGATTCGGTCGGTACTCGGCAACCAGGTGCTGCGACACAATGCCAACCAGTTGCTGCGCAAATTCCCCAAGCTGCACCATCAGTTGAAACTGTTCGCTCAGAACCGCGGGCTTATTGGTGGCGGTGCGTTCGCATCGGCG  >  NC_002947/1989652‑1989944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑