| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 197,632 | T→C | 24.1% | T1046T (ACT→ACC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 197,632 | 0 | T | C | 24.1% | 100.5 / 27.9 | 58 | T1046T (ACT→ACC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base T (23/21); new base C (9/5); total (32/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGT > NC_002947/197487‑197778 | acacCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGt > 1:204357/1‑148 (MQ=21) cacCTTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCa < 5:176243/149‑1 (MQ=21) tGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGt > 4:212731/1‑148 (MQ=39) tGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGt < 3:212731/148‑1 (MQ=39) tCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCAc > 6:223239/1‑148 (MQ=38) aGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCATCGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCCGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCga < 5:75989/149‑1 (MQ=255) aGCAATCATGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATc < 4:160627/147‑1 (MQ=37) aaTCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGaa > 4:132136/1‑149 (MQ=17) tCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGTGAATGACGTCTACAACAACGGGTCGACCGTCAGCGTCACCATCGAGAACg < 5:223361/149‑1 (MQ=12) tCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACg > 1:65695/1‑149 (MQ=17) cTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGc < 6:60761/148‑1 (MQ=17) cTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGc > 5:60761/1‑148 (MQ=17) cTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGc > 3:61823/1‑148 (MQ=17) cAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCGAGACCCCGGCCAATGACGTCTACAACAACGGGTCGACTGTCAGCGTCACCATCGAGAACGCCACAgg > 8:180088/1‑149 (MQ=11) gACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGC‑g > 3:120173/1‑149 (MQ=17) aCGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGC‑gg > 5:31190/1‑149 (MQ=17) gCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATc > 1:42736/1‑130 (MQ=17) gCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATc < 2:42736/130‑1 (MQ=17) cGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAAt > 4:112576/1‑149 (MQ=17) tAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTc < 2:67413/149‑1 (MQ=14) aCCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACATCAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGa < 6:32129/149‑1 (MQ=255) aCCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCg < 1:22983/148‑1 (MQ=255) aCCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGa < 2:53885/149‑1 (MQ=34) aCCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGa < 5:223239/149‑1 (MQ=34) cGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCac < 3:156134/120‑2 (MQ=2) cGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTAACCac > 4:156134/1‑119 (MQ=11) aCCCTGTCCAACGGCCACACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGCCCCCGGCGACTGAAGTCTACCACAACGGTTCGACTGTCAGCGGCCCCATCGCGAACGCCCCCGGC‑GGGAATTTCgcgcccc > 7:247392/1‑145 (MQ=11) aCCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGa > 8:101263/1‑143 (MQ=32) aCCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGa < 7:101263/143‑1 (MQ=32) cccTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTg > 8:87098/1‑149 (MQ=32) cGGCCAAACCATTACCGTTGAAACCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGACCCCGAAcc > 7:168136/1‑149 (MQ=18) gCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGACCCCGAACCCg < 7:160652/149‑1 (MQ=255) aaCGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGACCCCGAACCCGAccc > 5:9953/1‑147 (MQ=255) cgaTTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGACCCCGAACCCGACCCCg < 4:21133/147‑1 (MQ=12) ttACCGTTGAAGCCGGCAAAACCCAGGGCAGCGGCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCt < 6:9953/149‑1 (MQ=255) ccGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCt > 6:135012/1‑146 (MQ=12) ttGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATAGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGAc > 8:231156/1‑148 (MQ=255) gCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCCGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGAt > 5:31840/1‑149 (MQ=37) ggCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCaa < 2:267344/149‑2 (MQ=9) ccAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACACCGGTTCGACTGTCAGCGTCACCATCGAGAACGCCCCAGCC‑GGTAATTTCGAGCAACTGACCCCGAACCCGACGCCCGCTCCGCCCCCCATCACCGACTCgg > 1:188776/1‑149 (MQ=14) ccAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCgg < 7:87098/149‑1 (MQ=39) ccAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCCGTCGACACCACTACTGCg < 6:31840/148‑1 (MQ=255) aggccccGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGACCCCGAACCCGACTCCGGCTCa < 8:33676/106‑1 (MQ=255) aggccccGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGACCCCGAACCCGACTCCGGCTCa > 7:33676/4‑109 (MQ=255) gACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGACCCCGAACCCg > 7:115540/1‑97 (MQ=37) gACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGACCCCGAACCCg < 8:115540/97‑1 (MQ=37) tGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGAcccc < 2:4425/78‑1 (MQ=34) tGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGAcccc > 1:4425/1‑78 (MQ=34) tCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGGTAATTTCGAGCTACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAg > 1:70714/1‑149 (MQ=255) caacaaCGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCg > 7:133334/1‑139 (MQ=255) caacaaCGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCg < 8:133334/139‑1 (MQ=255) aacGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCg > 4:17945/1‑148 (MQ=255) acGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGaa < 8:168136/149‑1 (MQ=255) cGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAg > 4:98839/1‑149 (MQ=255) aCTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGa > 5:149363/1‑136 (MQ=255) aCTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGa < 6:149363/136‑1 (MQ=255) aCTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGt > 7:133477/1‑149 (MQ=255) cTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGt > 2:54093/1‑148 (MQ=255) | ACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGC‑GGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGT > NC_002947/197487‑197778 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |