Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,993,603 C→G 7.3% intergenic (+41/+57) PP_1780 → / ← PP_1781 mannosyltransferase/acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,993,6030CG 7.3% 170.0 / 2.2 54intergenic (+41/+57)PP_1780/PP_1781mannosyltransferase/acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (26/24);  new base G (2/2);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01

TGCCTTGGCTACATTGGAGTGAAAGTGCAGCTGAACTAAAACGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGGCGCAGATAGCGTTGAGCCGGGCGTTCCAGCCAGCGGTGGGTCAACAGGCTTAGCGGG  >  NC_002947/1993456‑1993750
                                                                                                                                                   |                                                                                                                                                   
tGCCTTGGCTACATTGGAGTGAAAGTGCAGCTGAACTAAAACGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCa                                                                                                                                                    <  1:173163/149‑1 (MQ=255)
       gCTACATTGGAGTGAAAGGGCAGCTGAACTAAAACGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCa                                                                                                                                              <  4:195567/148‑1 (MQ=255)
        cTACATTGGAGTGAAAGTGCAGCTGAACTAAAACGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCTGCGCCGCACt                                                                                                                                            <  1:45622/149‑1 (MQ=255)
            aTTGGAGTGAAAGTCCAGCTGAACTAAAACGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTAAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTCCAg                                                                                                                                        >  2:55466/1‑149 (MQ=255)
             ttGGAGTGAAAGTGCAGCTGAACTAAAACGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAgg                                                                                                                                       >  2:23077/1‑149 (MQ=255)
                gattGAAAGTGCAGCTGAACTAAAACGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCTGCGCCGCACTGc                                                                                                                                          <  4:53709/140‑1 (MQ=255)
                gAGTGAAAGTGCAGCTGAACTAAAACGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCTGCGCCGCACTGc                                                                                                                                          >  3:53709/1‑143 (MQ=255)
                     aaaGTGCAGCTGAACTAAAACGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTCAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGAACGCTAGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTg                                                                                                                                >  3:199629/1‑148 (MQ=255)
                      aaGTGCAGCTGAACTAAAACGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTTAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGcc                                                                                                                              <  7:40645/149‑1 (MQ=255)
                                  aCTAAAACGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTa                                                                                                                  <  3:26751/149‑1 (MQ=255)
                                  aCTAAAACGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTa                                                                                                                  <  1:155422/149‑1 (MQ=255)
                                    tAAAACGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCa                                                                                                                                         <  6:30718/124‑1 (MQ=255)
                                    tAAAACGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCa                                                                                                                                         <  6:26686/124‑1 (MQ=255)
                                    tAAAACGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCa                                                                                                                                         >  5:30718/1‑124 (MQ=255)
                                    tAAAACGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCa                                                                                                                                         >  5:26686/1‑124 (MQ=255)
                                    tAAAACGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTAAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTAt                                                                                                                 >  8:146314/1‑148 (MQ=255)
                                             tGGTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACg                                                                                                         >  1:239920/1‑147 (MQ=255)
                                                  ttGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCa                                                                                                                             >  3:216228/1‑122 (MQ=255)
                                                  ttGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCa                                                                                                                             <  4:216228/122‑1 (MQ=255)
                                                  ttGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTaaa                                                                                                  >  2:61402/1‑149 (MQ=255)
                                                    ggATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCtttt                                                                                                                                 >  1:228937/1‑116 (MQ=255)
                                                    ggATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCtttt                                                                                                                                 <  2:228937/116‑1 (MQ=255)
                                                    ggATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAAcg                                                                                                >  3:108243/1‑149 (MQ=255)
                                                      aTGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAAcgcg                                                                                              <  3:5473/149‑1 (MQ=255)
                                                            cGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTAt                                                                                         <  2:239920/148‑1 (MQ=255)
                                                                 aaTACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGGGGCGCAGCCGCACTGCAGGTCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCt                                                                                   <  6:252987/149‑1 (MQ=255)
                                                                      tCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATcc                                                                                       <  1:239331/140‑1 (MQ=255)
                                                                      tCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATcc                                                                                       >  2:239331/1‑140 (MQ=255)
                                                                           tGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAg                                                                         >  8:95024/1‑149 (MQ=255)
                                                                             aaGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGc                                                                                                                                                  <  5:5007/74‑1 (MQ=255)
                                                                             aaGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGc                                                                                                                                                  >  6:5007/1‑74 (MQ=255)
                                                                               gCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTg                                                                     >  4:81574/1‑149 (MQ=255)
                                                                               gCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTg                                                                     >  5:173982/1‑149 (MQ=255)
                                                                                cATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTgg                                                                    <  1:61402/149‑1 (MQ=255)
                                                                                  tAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGAAAGCAGTAAACGCGCTTATCCGGCTACGGGAACAGACTGGc                                                                   >  3:7533/1‑148 (MQ=255)
                                                                                      gcgcCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACt                                                                                                                      <  8:222721/93‑1 (MQ=255)
                                                                                      gcgcCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACt                                                                                                                      >  7:222721/1‑93 (MQ=255)
                                                                                       cgcCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCt                                                             <  3:81574/149‑1 (MQ=255)
                                                                                          cTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGgc                                                          >  3:190790/1‑149 (MQ=255)
                                                                                             cTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGGCGCa                                                       <  4:108243/149‑1 (MQ=255)
                                                                                              tCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGgcg                                                         >  2:237716/1‑146 (MQ=255)
                                                                                                aaGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGGCGCAGa                                                     >  2:226232/1‑148 (MQ=255)
                                                                                                      aCTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGGCGCa                                                       <  1:64438/140‑1 (MQ=255)
                                                                                                      aCTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGGCGCa                                                       >  2:64438/1‑140 (MQ=255)
                                                                                                      aCTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGGCGCAGATAGCGtt                                              <  7:43913/149‑1 (MQ=255)
                                                                                                              cGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGGCGCAGATAGCGTTGAGCCggg                                      >  8:35648/1‑149 (MQ=255)
                                                                                                                tACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGGCGCAGATAGCGTTGAGCCGGGCg                                    >  3:199895/1‑149 (MQ=255)
                                                                                                                              catGAACCCTGTAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGGCGCAGATAGCGTTGAGCCGGGCGTTCCAGCCAGCGGt                      <  1:226232/149‑1 (MQ=255)
                                                                                                                              catGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGGCGCAGATAGCGTTGAGCCGGGCGTTCCAGCCAGCGGt                      >  8:210023/1‑149 (MQ=255)
                                                                                                                               atGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGGCGCAGATAGCGTTGAGCCGGGCGTTCCAGCCAGCGGTg                     <  7:29375/149‑1 (MQ=255)
                                                                                                                                tGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGGCGCAGATAGCGTTGAGCCGGGCGTTCCAGCCAGCGGTgg                    <  7:35648/149‑1 (MQ=255)
                                                                                                                                      cTGCAGTGCGGCTGCGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGGCGCAGATAGCGTTGAGCCGGGCGTTCCAGCCAGCGGTGGGTCaa               >  7:13080/1‑148 (MQ=255)
                                                                                                                                      cTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGGCGCAGATAGCGTTGAGCCGGGCGTTCCAGCCAGCGGTgg                    >  3:174360/1‑143 (MQ=255)
                                                                                                                                      cTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGGCGCAGATAGCGTTGAGCCGGGCGTTCCAGCCAGCGGTgg                    <  4:174360/143‑1 (MQ=255)
                                                                                                                                                  gcAGCCGCACTGCAGGGCTTTTGCCTTATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGTCGCTGGCGCAGATAGCGTTGAGCCGGGCGTTCCAGCCAGCGGTGGGTCAACAGGCTTAGCggg  <  1:55466/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGCCTTGGCTACATTGGAGTGAAAGTGCAGCTGAACTAAAACGCCTGGTGTTGGATGTTACGGAAAATACTCAGCTGAAGCATAAGGCGCCTGCTCAAGCGCACTGATTGCGTACGCTTGGCCATCCATGAACCCTGCAGTGCGGCGCAGCCGCACTGCAGGGCTTTTGCCATATGACTTGTATGAGGCACGCAGTAAACGCGCTTATCCGGCTACGGGCACAGCCTGGCGACGCTGGCGCAGATAGCGTTGAGCCGGGCGTTCCAGCCAGCGGTGGGTCAACAGGCTTAGCGGG  >  NC_002947/1993456‑1993750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑