Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,013,530 C→T 9.0% L164L (TTG→TTA)  PP_1793 ← group 2 family glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,013,5300CT 9.0% 119.7 / 2.7 45L164L (TTG→TTA) PP_1793group 2 family glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base C (19/21);  new base T (2/2);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01

CTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTC‑TGTGTGTCTCCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGCGGGGGGCTCGGACTGGCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTG  >  NC_002947/2013383‑2013678
                                                                                                                                                    |                                                                                                                                                    
cTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTC‑TGTGTGTCTCCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGCCTTCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGTCGCATTGATCTCAGGc                                                                                                                                                      <  7:237718/148‑1 (MQ=255)
cTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTC‑TGTGTGTCTCCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGc                                                                                                                                                      >  1:159894/1‑148 (MQ=255)
     ggTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTC‑TGTGTGTCTCCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTgg                                                                                                                                                <  1:17719/149‑1 (MQ=255)
      gTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTCTTGGGTGTCTCCCGGTACCGCCCCCCCCGTATCCTACGAGGGGCGTCGGCCTGCGCCGCGCGCAGCTGGATCACTTGGGGTAATTCTTTGGGCGAATTGATGTCGGGAAAGTgg                                                                                                                                                >  1:180140/1‑149 (MQ=255)
           cAAGGTCAATGGCCCATCCCTGGATCTCTC‑TGTGTGTCTCCAGGTACCGCACCACACCTATCCTACGATGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGa                                                                                                                                          <  3:216476/149‑1 (MQ=255)
            aaGGTCAATGGCCCATCCCTGGATCTCTC‑TGTGTGTCTCCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCaa                                                                                                                                            >  3:105055/1‑146 (MQ=255)
            aaGGTCAATGGCCCATCCCTGGATCTCTC‑TGTGTGTCTCCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCaa                                                                                                                                            <  4:105055/146‑1 (MQ=255)
                tCAATGGCCCATCCCTGGATCTCTC‑TGTGTGTCTCCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCAt                                                                                                                                     >  8:183381/1‑149 (MQ=255)
                 cAATGGCCCATCCCTGGATCTCTC‑TGTGTGTCTCCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCAtg                                                                                                                                    <  6:134682/149‑1 (MQ=255)
                       cccATCCCTGGATCTCTC‑TGTGTGTCTCCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTgcg                                                                                                                               >  5:254780/1‑148 (MQ=255)
                               tGGATCTCTC‑TGTGTGTCTCCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGc                                                                                                                      >  6:128845/1‑149 (MQ=255)
                                      ctc‑tGTGTGTCTCCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTTGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGCgggggg                                                                                                                <  8:70747/148‑1 (MQ=255)
                                       tc‑tGTGTGTCTCCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGCgggg                                                                                                                  <  2:180140/145‑1 (MQ=255)
                                       tc‑tGTGTGTCTCCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGCggg                                                                                                                   <  7:177366/144‑1 (MQ=255)
                                       tc‑tGTGTGTCTCCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGCGGGGGGCt                                                                                                              >  8:77015/1‑149 (MQ=255)
                                       tc‑tGTGTGTCTCCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGCGGGGGGCt                                                                                                              >  8:20313/1‑149 (MQ=255)
                                            tgtgtCTCCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGCGGGGGGCTCg                                                                                                            <  7:183381/147‑1 (MQ=255)
                                              tgtCTCCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGCGGGGGGCTCGGACt                                                                                                        <  8:168853/149‑1 (MQ=255)
                                               gtCTCCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTgg                                                                                                                       <  2:178128/133‑1 (MQ=255)
                                               gtCTCCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTgg                                                                                                                       >  1:178128/1‑133 (MQ=255)
                                                tctcCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGCCTGTGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGCGGGGGGCTCGGACTgg                                                                                                      <  3:132416/149‑1 (MQ=255)
                                                  tcCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGCGGGGGGCTCGGACTGGc                                                                                                     >  3:74663/1‑148 (MQ=255)
                                                           cGCACCACACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTTCGCAGAGTGGCGGGGGTCTCGGACTGGCTACGtgactg                                                                                           >  6:254854/1‑149 (MQ=255)
                                                             caccacACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCa                                                                                                                             >  8:154497/1‑113 (MQ=255)
                                                             caccacACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCa                                                                                                                             <  7:154497/113‑1 (MQ=255)
                                                                   acCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGCGGGGGGCTCGGACTGGCTACGTGCCTGCCAAagca                                                                                   <  7:77015/149‑1 (MQ=255)
                                                                     cTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTATTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTGTC‑GGGGGCTCGGACTGGCTACGTGACTGCCAAAgcagca                                                                                >  4:135185/1‑149 (MQ=255)
                                                                           tACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGCGGGGGGCTCGGACTGGCTACGTGCCTGCCAAAGCAGCATACg                                                                            >  5:129822/1‑148 (MQ=255)
                                                                             cGAGGGGCGTCGGCCTGCGCAGCGATCAGCTTGATCACTTGGGATAATTATTTGGGCGCATTGATATAAGTCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGCGGGGGGCTCGGACTGGCTACGTGCCTGCCAAAGCAGCATACGc                                                                           >  6:198847/1‑147 (MQ=255)
                                                                                 gggCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGCGGGGGGCTCGGACTGGCTACGTGCCTGCCAAAGCAGCATACGCAaggag                                                                     <  4:74663/149‑1 (MQ=255)
                                                                                   gCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGCGGGGGGCTCGGACTGGCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGc                                                                   <  6:129822/149‑1 (MQ=255)
                                                                                   gCGTCGGCCTGCGCCGCGAGCAGCTTGATCACCTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGCGGGGGGCTCGGACTGGCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGc                                                                   <  6:182271/149‑1 (MQ=255)
                                                                                   gCGTCGGCCTGCGCCGCGAGCAGCTTGATCACCTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGCGGGGGGCTCGGACTGGCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGc                                                                   >  5:182271/1‑149 (MQ=255)
                                                                                     gTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGCGGGGGGCTCGGACTGGCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGt                                                                 <  6:254780/149‑1 (MQ=255)
                                                                                         gCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGCGGGGGGCTCGGACTGGCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATc                                                             <  2:159894/149‑1 (MQ=255)
                                                                                                       cagcTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGCGGGGGGCTCGGACTGGCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATCGGGTAGTTTCTGc                                                >  8:237052/1‑148 (MQ=255)
                                                                                                          cTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGTAAGTGGGCAAGAATc                                                                                                                                       <  7:10742/58‑1 (MQ=255)
                                                                                                          cTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGTAAGTGGGCAAGAATc                                                                                                                                       >  8:10742/1‑58 (MQ=255)
                                                                                                          cTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGTAAGTGGGCAAGAATc                                                                                                                                       >  4:24755/1‑58 (MQ=255)
                                                                                                          cTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGTAAGTGGGCAAGAATc                                                                                                                                       <  3:24755/58‑1 (MQ=255)
                                                                                                                               tttGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGCGGGGGGCTCGGACTGGCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTgg                       >  4:179596/1‑149 (MQ=255)
                                                                                                                               tttGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGCGGGGGGCTCGGACTGGCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTgg                       >  4:128618/1‑149 (MQ=255)
                                                                                                                                    gcgcATTGATCTCATGCTAGTGGGCAAGAATCATGCTGCGCAGTGTGGCGGGGGGCTCTGACTGTCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAg                   <  5:254854/148‑1 (MQ=255)
                                                                                                                                    gcgcATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGCGGGGGGCTCGGACTGGCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATCGTGTAGTTTCTGCCATGCATGAGTGAGGTATCTGGTGGCGAg                   >  3:138945/1‑148 (MQ=255)
                                                                                                                                        aTTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGC‑GGGGGCTCGGACTGGCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGc             <  3:135185/149‑1 (MQ=255)
                                                                                                                                                   gCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGCGGGGGGCTCGGACTGGCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGa                                   <  6:226635/117‑1 (MQ=255)
                                                                                                                                                   gCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGCGGGGGGCTCGGACTGGCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGa                                   >  5:226635/1‑117 (MQ=255)
                                                                                                                                                    cAAGTTGGCAAGAATCATGCTGCGCAGTGTGGCGGTGGTCGAGGACTGGATCCGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATCGGGTAGTTTATGCCATGCCTGCGTGAGGTATCGGTGGGCGAGCACTGCAAGCCCTtgtg  <  4:138945/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTGCAGGTTGCCAAGGTCAATGGCCCATCCCTGGATCTCTC‑TGTGTGTCTCCAGGTACCGCACCACACCTATCCTACGAGGGGCGTCGGCCTGCGCCGCGAGCAGCTTGATCACTTGGGCTAATTCTTTGGGCGCATTGATCTCAGGCAAGTGGGCAAGAATCATGCTGCGCAGTGTGGCGGGGGGCTCGGACTGGCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTG  >  NC_002947/2013383‑2013678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑