| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,064,594 | T→A | 11.1% | Q135L (CAG→CTG) | PP_1841 ← | cytochrome c family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,064,594 | 0 | T | A | 11.1% | 92.2 / 3.8 | 36 | Q135L (CAG→CTG) | PP_1841 | cytochrome c family protein |
| Reads supporting (aligned to +/- strand): ref base T (18/14); new base A (2/2); total (20/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 | |||||||||||
GGGCAAAGCCCTGGCGCACCGGGCTGTCGGCCGGCAGCTTCGGGTCGGGCAGCAGCGCAGGAAACCGCTGGTCCACCGGGGCCAGCTTGCGAAGGGTGGCAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCAGGTAGAACGGCCCGGCACTTGGCTTGCCTTGACCCAGTGGCGGCCACGGCTTGCCTGGGTCTTCCACGGCCAACCAGGCCCGTGCTGGCCCTGGCTGCAGCAATGCCGCAGCGGGCATTTCGGCGGCA > NC_002947/2064454‑2064740 | gggCAAAGCCCTGGCGCACCGGGCTGTCGGCCGGCAGCTTCGGGTCGGGCAGCAGCGCAGGAAACCGCTGGTCCACCGGGGCCAGCTTGCGAAGGGTGGCAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGCAGGCCAgcggca > 7:185481/1‑146 (MQ=255)gggCAAAGCCCTGGCGCACCGGGCTGTCGGCCGGCAGCTTCGGGTCGGGCAGCAGCGCAGGAAACCGCTGGTCCACCGGGGCCAGCTTGCGAAGGGTGGCAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGCAGGCCAgcggca < 8:185481/147‑2 (MQ=255) ggCAAAGCCCTGGCGCACCGGGCTGTCGGCCGGCAGCTTCGGGTCGGGCAGCAGCGCAGGAAACCGCTGGTCCACCGGGGCCAGCTTGCGAAGGGTGGCAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAg > 6:269196/1‑149 (MQ=255) aaGCCCTGGCGCACCGGGCTGTCGGCCGGCAGCTTCGGGTCGGGCAGCAGCGCAGGAAACCGCTGGTCCACCGGGGCCAGCTTGCGAAGGGTGGCAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCca < 2:102190/149‑1 (MQ=255) gcACCGGGCTGTCGGCCGGCAGATTCGCGTCGGGCAGCAGCGCAGGAAACCGCTGGTCCACCGGCGCCAGCTTGCGAAGGGTGGCAATCTGCAATGGCCACTGTGCAGGGCGGATCCCGCTGCCCTGCGGCGCAGTCCCCACCAgg > 2:211039/1‑146 (MQ=255) cACCGGGCTGTCGGCCGGCAGCTTCGGGTCGGGCAGCAGCGCAGGAAACCGCTGGTCCACCGGGGCCAGCTTGCGAAGGGTGGCAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCAGGTAg > 8:233436/1‑148 (MQ=255) aCCGGGCTGTCGGCCGGCAGCTTCGGGTCGGGCAGCAGCGCAGGAAACCGCTGGTCCACCGGGGCCAGCTTGCGAAGGGTGGCAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCAGGTAGaa < 7:233436/149‑1 (MQ=255) gCTGTCGGCCGGCAGCTTCGGGTCGGGCAGCAGCGCAGGAAACCGCTGGTCCACCGGGGCCAGCTTGCGAAGGGTGGCAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCAGGTAg > 6:109402/1‑142 (MQ=255) gCTGTCGGCCGGCAGCTTCGGGTCGGGCAGCAGCGCAGGAAACCGCTGGTCCACCGGGGCCAGCTTGCGAAGGGTGGCAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCAGGTAg < 5:109402/142‑1 (MQ=255) tCGGCCGGCAGCTTCGGGTCGGGCAGCAGCGCAGGAAACCGCTGGTCCACCGGGGCCAGCTTGCGAAGGGTGGCAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCAGGTAGAACGGCCCg < 5:71165/147‑1 (MQ=255) cggccggcAGCTTCGGGTCGGGCAGCAGCGCAGGAAACCGCTGGTCCACCGGGGCCAGCTTGCGAAGGGTGGCAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCAGGTAGAACGGCCCGGCa > 6:271752/1‑149 (MQ=255) ggccggcAGCTTCGGGTCGGGCAGCAGCGCAGGAAACCGCTGGTCCACCGGGGCCAGCTTGCGAAGGGTGGCAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGCAGGCCAgcggca < 3:193306/119‑2 (MQ=255) ggccggcAGCTTCGGGTCGGGCAGCAGCGCAGGAAACCGCTGGTCCACCGGGGCCAGCTTGCGAAGGGTGGCAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGCAGGCCAgcggca > 4:193306/1‑118 (MQ=255) cggcAGCTTCGGGTCGGGCAGCAGCGCAGGAAACCGCTGGTCCACCGGGGCCAGCTTGCGAAGGGTGGCAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCAGGTAGCACGGCCCGGCCCtgg > 4:159683/1‑147 (MQ=255) ggtcgggCAGCAGCGCAGGAAACCGCTGGTCCACCGGGGCCAGCTTGCGAAGGGTGGCAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCAGGTAGAACGGCCCGGCACTTGGCTTGCCTTGa > 1:246117/1‑149 (MQ=255) ggCAGCAGCGCAGGAAACCGCTGGTCCACCGGGGCCAGCTTGCGAAGGGTGGCAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCAGGTAGAACGGCCCGGCACTTGGCTTGCCTTGACCCAg > 4:48301/1‑149 (MQ=255) agcagcGCGGGAAACCGCTGGTCGACCGGGGCCAGCTTGCGAAGGGTGGCAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCAGGTAGAACGGCCCGGCACTTGGCTTGCCTTGACCCAGTgg > 7:90318/1‑149 (MQ=255) gcgcAGGAAACCGCTGGTCCACCGGGGCCAGCTTGCGAAGGGTGGCAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCAGGTAGAACGGCCCGGCACTTGGCTTGCCTTGACCCAGTggcggc < 1:50999/149‑1 (MQ=255) gAAACCGCTGGTCCACCGGGGCCAGCTTGCGAAGGGTGGCAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCAGGTAGAACGGCCCGGCACTTGGCTTGCCTTGACCCAGTGGCGGCCACGGc > 2:21500/1‑149 (MQ=255) aaCCGCTGGTCCACCGGGGCCAGCTTGCGAAGGGTGGCAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCAGGTAGAACGGCCCGGCACTTGGCTTGCCTTGACCCAGTGGCGGCCACGGCtt > 4:212458/1‑149 (MQ=255) cGGGGCCAGCTTGCGAAGGGTGGCAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCAGGTAGAACGGCCCGGCACTTGGCTTGCCTTGACCCAGTGGCGGCCACGGCTTGCCTGGGTCTTCCa > 7:159063/1‑149 (MQ=255) cTTGCGAAGGGTGGCAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCAGGTAGAACGGCCCGGCACTTGGCTTGCCTTGACCCAGTGGCGGCCACGGCTTGCCTGGGTCTTCCACGGCCAAcc < 5:271752/149‑1 (MQ=255) gCGAAGGGTGGCAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCAGGTAGAACGGCCCGGCACTTGGCTTGCCTTGACCCAGTGGCGGCCACGGCTTGCCTGGGTCTTCCACGGCCAACCAg < 3:48301/148‑1 (MQ=255) gAAGGGTGGCAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCAGGTAGAACGGCCCGGCACTTGGCTTGCCTTGACCCAGTGGCGGCCACGGCTTGCCTGGGTCTTCCACGGCCAACCAGGcc > 5:81039/1‑149 (MQ=255) gTGGCAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGGTGGCCTGCGGCGCAGTCCACACCAGGTAGAACGGCCCAGCACTTGGCTTGCCTTGACCCAGTGGCGGCCACGGCTTGCCTGGGTCTTCCACGGCCAACCAGGCCCGTg < 1:211039/148‑1 (MQ=255) cAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCAGGTAGAACGGCCCGGCACTTGGCTTGCCTTGACCCAGTGGCGGCCACGGCTTGCCTGGGTCTTCCACGGCCAACCAGGCCCGTGCTGGc < 6:81039/149‑1 (MQ=255) ggAATGGCCACTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCAGGTAGAACGGCCCGGCACTTGGCTTGCCTTGACCCAGTGGCGGCCACGGCTTGCCTGGGTCTTCCACGGCCAACCAGGCCCGTGCTGGCCCTGGc > 6:184743/1‑149 (MQ=255) gAATGGCCACTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCAGGTAGAACGGCCCGGCACTTGGCTTGCCTTGACCCAGTGGCGGCCACGGCTTGCCTGGGTCTTCCACGGCCAACCAGGCCCGTGCTGGCCCt < 8:159063/145‑1 (MQ=255) gAATGGCCACTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCAGGTAGAACGGCCCGGCACTTGGCTTGCCTTGACCCAGTGGCGGCCACGGCTTGCCTGGGTCTTCCACGGCCAACCAGGCCCGTGCTGGCCCTgg < 2:246117/147‑1 (MQ=255) tGGCCACTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCAGGTAGAACGGCCCGGCACTTGGCTTGCCTTGACCCAGTGGCGGCCACGGCTTGCCTGGGTCTTCCACGCCCAACCAGGCCCGTGCTGGCCCTGGCTgca < 1:21500/149‑1 (MQ=255) aCTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCAGGTAGAACGGCCCGGCACTTGGCTTGCCTTGACCCAGTGGCGGCCACGGCTTGCCTGGGTCTTCCACGGCCAACCAGGCCCGTGCTGGCCCTGGCTGCAGCAAt < 8:90318/149‑1 (MQ=255) aCTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCACGTAGAACGGCCCGGCACTTGGCTTGCCTTGACCCAGTGGCGGCCACGGCTTGCCTGGGTCTTCCACGGCCAACCAGGCCCGTGCTGGCCCTGGCTGCAGCAAt > 3:99059/1‑149 (MQ=255) aGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCAGGTAGAACGGCCCGGCACTTGGCTTGCCTTGACCCAGTGGCGGCCACGGCTTGCCTGGGTCTTCCACGGCCAACCAGGCCCGTGCTGGCACTGGCTGCAGCAATGCCGCAg > 3:56636/1‑149 (MQ=255) tggtgtGGACTGCGCCGCAGGCCACACCAGGTAGAACGGCCCGGCACTTGGCTTGCCTTGACCCAGTGGCGGCCACGGCTTGCCTGGGTCTTCCACGGCCAACCAGGCCCGTGCTGGCCCTGGCTGCAGCAATGCCGCAGCGGGCAttt > 6:60330/6‑149 (MQ=255) cTGGCCTGCGGCGCAGTCCACACCAGGTAGAACGGCCCGGCACTTGGCTTGCCTTGACCCAGTGGCGGCCACGGCTTGCCTGGGTCTTCCACGGCCAACCAGGCCCGTGCTGGCCCTGGCTGCAGCAATGCCGCAGCGGGCATTTcggc < 5:269196/149‑1 (MQ=255) cTGCGGCGCAGTCCACACCAGGTAGAACGGCCCGGCACTTGGCTTGCCTTGACCCAGTGGCGGCCACGGCTTGCCTGGGTCTTCCACGGCCAACCAGGCCCGTGCTGGCCCTGGCTGCAGCAATGCCGCAGCGGGCATTTCGGCGGCa > 8:129110/1‑148 (MQ=255) | GGGCAAAGCCCTGGCGCACCGGGCTGTCGGCCGGCAGCTTCGGGTCGGGCAGCAGCGCAGGAAACCGCTGGTCCACCGGGGCCAGCTTGCGAAGGGTGGCAATCTGGAATGGCCACTGTTCAGGGCGGATGCCGCTGGCCTGCGGCGCAGTCCACACCAGGTAGAACGGCCCGGCACTTGGCTTGCCTTGACCCAGTGGCGGCCACGGCTTGCCTGGGTCTTCCACGGCCAACCAGGCCCGTGCTGGCCCTGGCTGCAGCAATGCCGCAGCGGGCATTTCGGCGGCA > NC_002947/2064454‑2064740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |