| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,113,676 | G→T | 11.1% | Q204K (CAG→AAG) | PP_1882 ← | membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,113,676 | 0 | G | T | 11.1% | 104.6 / 4.9 | 36 | Q204K (CAG→AAG) | PP_1882 | membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (17/15); new base T (2/2); total (19/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01 | |||||||||||
GCTCACGCACAACAACCCGTTCACCAGGGCGTATTCCAGGCGCCAGCTGGCCAGCTCATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGAGGTCGATCCTGTGCCCCGTGGCC > NC_002947/2113533‑2113821 | gCTCACGCACAACAACCCGTTCACCAGGGCGTATTCCAGGCGCCAGCTGGCCAGCTCATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATaa < 2:86426/148‑1 (MQ=255) aCCAGGGCGTATTCCAGGCGCCAGCTGGCCAGCTCATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTTATCAGGgcgac < 4:195100/133‑3 (MQ=255) aCCAGGGCGTATTCCAGGCGCCAGCTGGCCAGCTCATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTTATCAGGgcgac > 3:195100/1‑131 (MQ=255) cAGGGCGTATTCCAGGCGCCAGCTGGCCAGCTCATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCACTTCGATGCGGc > 2:270697/1‑149 (MQ=255) ggCGTATTCCAGGCGCCAGCTGGCCAGCTCATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTAt > 2:14477/1‑149 (MQ=255) cGTATTCCAGGCGCCAGCTGGCCAGCTCATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTAtcg > 2:123493/1‑149 (MQ=255) aGGCGCCAGCTGGCCAGCTCATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTc > 4:5488/1‑149 (MQ=255) gcCAGCTGGCCAGCTCATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGAtt < 1:123493/149‑1 (MQ=255) aGCTGGCCAGCTCATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTc > 1:126525/1‑148 (MQ=255) gCCAGCTCATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCcagc < 1:14477/147‑1 (MQ=255) gCTCATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGAtc > 7:37931/1‑149 (MQ=255) tcgatGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATc < 2:126525/149‑1 (MQ=255) cgatGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCACCTCACGCTCGTCGCTGATc < 8:37931/148‑1 (MQ=255) atGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAg < 6:177960/149‑1 (MQ=255) ccAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGca > 1:155199/1‑149 (MQ=255) aGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGcatc < 5:48231/149‑1 (MQ=255) tCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTACAGCAGCATATCCAAACGCTCGTAGCTGATCGAGCTGAGcatcat > 4:64952/1‑149 (MQ=255) aGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGt < 2:155199/148‑1 (MQ=255) gTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGc > 7:161068/1‑116 (MQ=255) gTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGc < 8:161068/116‑1 (MQ=255) gCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGAAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTTATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGtt < 3:64952/148‑1 (MQ=255) cATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAATGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTaa > 8:31194/1‑131 (MQ=255) cATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTaa < 7:31194/131‑1 (MQ=255) cATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTgg > 2:3982/1‑149 (MQ=255) tAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTa < 3:5488/149‑1 (MQ=255) aaaGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAg < 5:161203/113‑1 (MQ=255) aaaGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAg > 6:161203/1‑113 (MQ=255) tGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCcagca < 1:75523/70‑1 (MQ=255) tGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCcagca > 2:75523/1‑70 (MQ=255) tGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGAGGTCGATCCt > 7:157280/1‑149 (MQ=255) cGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTg > 6:223807/1‑98 (MQ=255) cGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTg < 5:223807/98‑1 (MQ=255) gagcGCCCTTATCAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGcatc > 3:31668/4‑101 (MQ=255) gagcGCCCTTATCAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGcatc < 4:31668/98‑1 (MQ=255) ccTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGAGGTCGATCCTGTGCCCCGTGGcc > 6:155191/1‑149 (MQ=255) ccTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCAATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGAGGTCGATCCTGTGCCCCGTGGcc > 6:44871/1‑149 (MQ=255) | GCTCACGCACAACAACCCGTTCACCAGGGCGTATTCCAGGCGCCAGCTGGCCAGCTCATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGAGGTCGATCCTGTGCCCCGTGGCC > NC_002947/2113533‑2113821 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |