Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,113,679 A→C 10.8% Y203D (TAT→GAT)  PP_1882 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,113,6790AC10.8% 91.4 / 3.1 36Y203D (TAT→GAT) PP_1882membrane protein
Reads supporting (aligned to +/- strand):  ref base A (17/15);  new base C (2/2);  total (19/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTCACGCACAACAACCCGTTCACCAGGGCGTATTCCAGGCGCCAGCTGGCCAGCTCATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGAGGTCGATCCTGTGCCCCGTGGCCGCGTA  >  NC_002947/2113533‑2113826
                                                                                                                                                  |                                                                                                                                                   
gCTCACGCACAACAACCCGTTCACCAGGGCGTATTCCAGGCGCCAGCTGGCCAGCTCATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATaa                                                                                                                                                    <  2:86426/148‑1 (MQ=255)
                      aCCAGGGCGTATTCCAGGCGCCAGCTGGCCAGCTCATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTTATCAGGgcgac                                                                                                                                             <  4:195100/133‑3 (MQ=255)
                      aCCAGGGCGTATTCCAGGCGCCAGCTGGCCAGCTCATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTTATCAGGgcgac                                                                                                                                             >  3:195100/1‑131 (MQ=255)
                        cAGGGCGTATTCCAGGCGCCAGCTGGCCAGCTCATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCACTTCGATGCGGc                                                                                                                           >  2:270697/1‑149 (MQ=255)
                           ggCGTATTCCAGGCGCCAGCTGGCCAGCTCATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTAt                                                                                                                        >  2:14477/1‑149 (MQ=255)
                             cGTATTCCAGGCGCCAGCTGGCCAGCTCATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTAtcg                                                                                                                      >  2:123493/1‑149 (MQ=255)
                                     aGGCGCCAGCTGGCCAGCTCATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTc                                                                                                              >  4:5488/1‑149 (MQ=255)
                                         gcCAGCTGGCCAGCTCATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGAtt                                                                                                          <  1:123493/149‑1 (MQ=255)
                                            aGCTGGCCAGCTCATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTc                                                                                                        >  1:126525/1‑148 (MQ=255)
                                                 gCCAGCTCATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCcagc                                                                                                    <  1:14477/147‑1 (MQ=255)
                                                     gCTCATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGAtc                                                                                              >  7:37931/1‑149 (MQ=255)
                                                                          tcgatGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATc                                                                         <  2:126525/149‑1 (MQ=255)
                                                                           cgatGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCACCTCACGCTCGTCGCTGATc                                                                         <  8:37931/148‑1 (MQ=255)
                                                                             atGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAg                                                                      <  6:177960/149‑1 (MQ=255)
                                                                                    ccAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGca                                                               >  1:155199/1‑149 (MQ=255)
                                                                                      aGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGcatc                                                             <  5:48231/149‑1 (MQ=255)
                                                                                        tCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTACAGCAGCATATCCAAACGCTCGTAGCTGATCGAGCTGAGcatcat                                                           >  4:64952/1‑149 (MQ=255)
                                                                                                    aGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGt                                                <  2:155199/148‑1 (MQ=255)
                                                                                                      gTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGc                                                                              <  8:161068/116‑1 (MQ=255)
                                                                                                      gTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGc                                                                              >  7:161068/1‑116 (MQ=255)
                                                                                                             gCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGAAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTTATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGtt                                       <  3:64952/148‑1 (MQ=255)
                                                                                                              cATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAATGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTaa                                                       >  8:31194/1‑131 (MQ=255)
                                                                                                              cATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTaa                                                       <  7:31194/131‑1 (MQ=255)
                                                                                                              cATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTgg                                     >  2:3982/1‑149 (MQ=255)
                                                                                                                tAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTa                                   <  3:5488/149‑1 (MQ=255)
                                                                                                                 aaaGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAg                                                                      >  6:161203/1‑113 (MQ=255)
                                                                                                                 aaaGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAg                                                                      <  5:161203/113‑1 (MQ=255)
                                                                                                                               tGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCcagca                                                                                                   >  2:75523/1‑70 (MQ=255)
                                                                                                                               tGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCcagca                                                                                                   <  1:75523/70‑1 (MQ=255)
                                                                                                                               tGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGAGGTCGATCCt                    >  7:157280/1‑149 (MQ=255)
                                                                                                                                   cGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTg                                                                   >  6:223807/1‑98 (MQ=255)
                                                                                                                                   cGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTg                                                                   <  5:223807/98‑1 (MQ=255)
                                                                                                                                      gagcGCCCTTATCAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGcatc                                                             <  4:31668/98‑1 (MQ=255)
                                                                                                                                      gagcGCCCTTATCAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGcatc                                                             >  3:31668/4‑101 (MQ=255)
                                                                                                                                            ccTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGAGGTCGATCCTGTGCCCCGTGGcc       >  6:155191/1‑149 (MQ=255)
                                                                                                                                            ccTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCAATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGAGGTCGATCCTGTGCCCCGTGGcc       >  6:44871/1‑149 (MQ=255)
                                                                                                                                                 tAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGAGGTCGATCCTGTGCCCCGTGGCCGCGTa  <  8:157280/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GCTCACGCACAACAACCCGTTCACCAGGGCGTATTCCAGGCGCCAGCTGGCCAGCTCATGGGTTGGCAGCACGATCGATGTGACCCAGTCCGTGTTGATCAGGTTCATCGCATAAAGGGCAACGGGCTGGCCGTTGTCGCCCTGATAAGGGCGCTCGCGCAATTCGATGCGGCTATCGTCGCGTTCGATTTCCAGCAGGATCTCCTCACGCTCGTCGCTGATCGAGCTGAGCATCATGTAAGACGAGTTGAACGGTTGGTACTGGAGGTCGATCCTGTGCCCCGTGGCCGCGTA  >  NC_002947/2113533‑2113826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑