Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,154,386 T→G 7.2% Q129P (CAG→CCG)  PP_1909 ← septum formation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,154,3860TG 7.2% 162.1 / 2.6 53Q129P (CAG→CCG) PP_1909septum formation protein
Reads supporting (aligned to +/- strand):  ref base T (25/24);  new base G (2/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGT  >  NC_002947/2154251‑2154530
                                                                                                                                       |                                                                                                                                                   
tGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGt                                                                                                                                        >  8:183019/1‑149 (MQ=255)
  gAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTgg                                                                                                                                      >  2:120503/1‑149 (MQ=255)
  gAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCGGGCAATGCCAGGTg                                                                                                                                       >  1:229117/1‑148 (MQ=255)
  gAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCGGGCAATGCCAGGTg                                                                                                                                       >  4:233827/1‑148 (MQ=255)
   aaCAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGgc                                                                                                                                     >  7:36011/1‑149 (MQ=255)
   aaCAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCGGGCAATGCCAGGTgga                                                                                                                                     <  2:229117/149‑2 (MQ=255)
     cAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCGGGCAATGCCAGGTggatt                                                                                                                                   <  3:233827/149‑4 (MQ=255)
      aaGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCt                                                                                                                                  <  8:56273/149‑1 (MQ=255)
        gCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGt                                                                                                                                <  7:96275/149‑1 (MQ=255)
            aCCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTcag                                                                                                                            <  6:86233/149‑1 (MQ=255)
            aCCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTcag                                                                                                                            <  2:143952/149‑1 (MQ=255)
              ccccAGCCCCTCCGCCTCGAAACTCCCTGCGCCATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTACAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTcagca                                                                                                                          <  2:206503/149‑1 (MQ=255)
                ccAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGTTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTgg                                                                                                                                      <  1:81124/135‑1 (MQ=255)
                ccAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTgg                                                                                                                                      >  2:81124/1‑135 (MQ=255)
                ccAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTcag                                                                                                                            <  2:101950/145‑1 (MQ=255)
                ccAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTcag                                                                                                                            >  1:101950/1‑145 (MQ=255)
                ccAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGt                                                                                                                        >  3:254946/1‑149 (MQ=255)
                 cAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTg                                                                                                                       >  1:88397/1‑149 (MQ=255)
                    ccccTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCACTGCTGAACAGCGCCa                                                                                                                    <  3:12276/149‑1 (MQ=255)
                      ccTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGc                                                                                                                  >  3:174101/1‑149 (MQ=255)
                       cTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTca                                                                                                                             >  1:122850/1‑137 (MQ=255)
                       cTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTca                                                                                                                             <  2:122850/137‑1 (MQ=255)
                           gCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGt                                                                                                           <  2:88397/149‑1 (MQ=255)
                                 aaaCTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGaaa                                                                                                     <  7:159854/149‑1 (MQ=255)
                                    cTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCt                                                                                                  <  7:183019/149‑1 (MQ=255)
                                       ccTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAc                                                                                               >  3:70474/1‑149 (MQ=255)
                                           cgcAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑ctgc                                                                                          >  1:35333/1‑149 (MQ=255)
                                                  aaGCGGCTGTTCTTCTGCCCCGTAACACTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGAACGCAATCCACCTGGCATTCCCCGGTGCCGCTGTTCAGCAGTCCCACAC‑‑AGGT‑AGAAACCTCAA‑CTGCTGCCCGCt                                                                                  >  8:240865/2‑149 (MQ=255)
                                                                     aCGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGtt                                                                    <  8:10223/145‑1 (MQ=255)
                                                                     aCGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGtt                                                                    >  7:10223/1‑145 (MQ=255)
                                                                      cGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCAc                                                                              <  8:86284/134‑1 (MQ=255)
                                                                      cGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCAc                                                                              >  7:86284/1‑134 (MQ=255)
                                                                      cGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTg                                                                   >  2:149092/1‑145 (MQ=255)
                                                                      cGTAACGCTCGACCTGCTACCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAATCTCACACTGCTGCCCGCTGCACTCCAGTAGTTg                                                                   <  1:149092/146‑1 (MQ=255)
                                                                        tAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGc                                                              >  6:132068/1‑148 (MQ=255)
                                                                               cGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGt                                                                                                                        >  4:20157/1‑86 (MQ=255)
                                                                               cGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGt                                                                                                                        <  3:20157/86‑1 (MQ=255)
                                                                                                aGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGc                                       <  2:35333/147‑1 (MQ=255)
                                                                                                aGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCtt                                     <  4:70474/149‑1 (MQ=255)
                                                                                                 gTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTg                                    >  8:138960/1‑149 (MQ=255)
                                                                                                 gTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTg                                    >  2:217241/1‑149 (MQ=255)
                                                                                                     aCGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGcc                                  >  1:56895/1‑147 (MQ=255)
                                                                                                     aCGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCa                                <  5:132068/149‑1 (MQ=255)
                                                                                                       gCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCAAGGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGcc                                  >  1:83595/1‑147 (MQ=255)
                                                                                                                cGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCt                          >  7:78797/1‑144 (MQ=255)
                                                                                                                cGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCt                          <  8:78797/144‑1 (MQ=255)
                                                                                                                  gTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGcc                   <  4:254946/149‑1 (MQ=255)
                                                                                                                       aGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATa              <  8:36011/149‑1 (MQ=255)
                                                                                                                         gCACGCAATCCTCCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATAcc            <  7:138960/149‑1 (MQ=255)
                                                                                                                           aCGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCg           >  3:185569/1‑148 (MQ=255)
                                                                                                                                   cACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGt  >  8:244054/1‑149 (MQ=255)
                                                                                                                                    aCCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTg    >  5:260039/1‑146 (MQ=255)
                                                                                                                                    aCCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTg    <  6:260039/146‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                                   
TGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGT  >  NC_002947/2154251‑2154530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑