Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,154,391 T→A 7.3% Q127H (CAA→CAT)  PP_1909 ← septum formation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,154,3910TA 7.3% 159.6 / 3.0 55Q127H (CAA→CAT) PP_1909septum formation protein
Reads supporting (aligned to +/- strand):  ref base T (26/25);  new base A (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-01

TGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTCCGAGCC  >  NC_002947/2154251‑2154537
                                                                                                                                            |                                                                                                                                                     
tGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGt                                                                                                                                               >  8:183019/1‑149 (MQ=255)
  gAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTgg                                                                                                                                             >  2:120503/1‑149 (MQ=255)
  gAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCGGGCAATGCCAGGTg                                                                                                                                              >  4:233827/1‑148 (MQ=255)
  gAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCGGGCAATGCCAGGTg                                                                                                                                              >  1:229117/1‑148 (MQ=255)
   aaCAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGgc                                                                                                                                            >  7:36011/1‑149 (MQ=255)
   aaCAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCGGGCAATGCCAGGTgga                                                                                                                                            <  2:229117/149‑2 (MQ=255)
     cAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCGGGCAATGCCAGGTggatt                                                                                                                                          <  3:233827/149‑4 (MQ=255)
      aaGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCt                                                                                                                                         <  8:56273/149‑1 (MQ=255)
        gCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGt                                                                                                                                       <  7:96275/149‑1 (MQ=255)
            aCCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTcag                                                                                                                                   <  2:143952/149‑1 (MQ=255)
            aCCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTcag                                                                                                                                   <  6:86233/149‑1 (MQ=255)
              ccccAGCCCCTCCGCCTCGAAACTCCCTGCGCCATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTACAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTcagca                                                                                                                                 <  2:206503/149‑1 (MQ=255)
                ccAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGTTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTgg                                                                                                                                             <  1:81124/135‑1 (MQ=255)
                ccAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTgg                                                                                                                                             >  2:81124/1‑135 (MQ=255)
                ccAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTcag                                                                                                                                   <  2:101950/145‑1 (MQ=255)
                ccAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTcag                                                                                                                                   >  1:101950/1‑145 (MQ=255)
                ccAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGt                                                                                                                               >  3:254946/1‑149 (MQ=255)
                 cAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTg                                                                                                                              >  1:88397/1‑149 (MQ=255)
                    ccccTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCACTGCTGAACAGCGCCa                                                                                                                           <  3:12276/149‑1 (MQ=255)
                      ccTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGc                                                                                                                         >  3:174101/1‑149 (MQ=255)
                       cTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTca                                                                                                                                    >  1:122850/1‑137 (MQ=255)
                       cTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTca                                                                                                                                    <  2:122850/137‑1 (MQ=255)
                           gCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGt                                                                                                                  <  2:88397/149‑1 (MQ=255)
                                 aaaCTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGaaa                                                                                                            <  7:159854/149‑1 (MQ=255)
                                    cTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCt                                                                                                         <  7:183019/149‑1 (MQ=255)
                                       ccTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAc                                                                                                      >  3:70474/1‑149 (MQ=255)
                                           cgcAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑ctgc                                                                                                 >  1:35333/1‑149 (MQ=255)
                                                  aaGCGGCTGTTCTTCTGCCCCGTAACACTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGAACGCAATCCACCTGGCATTCCCCGGTGCCGCTGTTCAGCAGTCCCACAC‑‑AGGT‑AGAAACCTCAA‑CTGCTGCCCGCt                                                                                         >  8:240865/2‑149 (MQ=255)
                                                                     aCGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGtt                                                                           <  8:10223/145‑1 (MQ=255)
                                                                     aCGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGtt                                                                           >  7:10223/1‑145 (MQ=255)
                                                                      cGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCAc                                                                                     <  8:86284/134‑1 (MQ=255)
                                                                      cGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCAc                                                                                     >  7:86284/1‑134 (MQ=255)
                                                                      cGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTg                                                                          >  2:149092/1‑145 (MQ=255)
                                                                      cGTAACGCTCGACCTGCTACCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAATCTCACACTGCTGCCCGCTGCACTCCAGTAGTTg                                                                          <  1:149092/146‑1 (MQ=255)
                                                                        tAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGc                                                                     >  6:132068/1‑148 (MQ=255)
                                                                               cGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGt                                                                                                                               >  4:20157/1‑86 (MQ=255)
                                                                               cGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGt                                                                                                                               <  3:20157/86‑1 (MQ=255)
                                                                                                aGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGc                                              <  2:35333/147‑1 (MQ=255)
                                                                                                aGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCtt                                            <  4:70474/149‑1 (MQ=255)
                                                                                                 gTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTg                                           >  8:138960/1‑149 (MQ=255)
                                                                                                 gTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTg                                           >  2:217241/1‑149 (MQ=255)
                                                                                                     aCGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGcc                                         >  1:56895/1‑147 (MQ=255)
                                                                                                     aCGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCa                                       <  5:132068/149‑1 (MQ=255)
                                                                                                       gCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCAAGGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGcc                                         >  1:83595/1‑147 (MQ=255)
                                                                                                                cGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCt                                 >  7:78797/1‑144 (MQ=255)
                                                                                                                cGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCt                                 <  8:78797/144‑1 (MQ=255)
                                                                                                                  gTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGcc                          <  4:254946/149‑1 (MQ=255)
                                                                                                                       aGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATa                     <  8:36011/149‑1 (MQ=255)
                                                                                                                         gCACGCAATCCTCCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATAcc                   <  7:138960/149‑1 (MQ=255)
                                                                                                                           aCGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCg                  >  3:185569/1‑148 (MQ=255)
                                                                                                                                   cACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGt         >  8:244054/1‑149 (MQ=255)
                                                                                                                                    aCCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTg           >  5:260039/1‑146 (MQ=255)
                                                                                                                                    aCCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTg           <  6:260039/146‑1 (MQ=255)
                                                                                                                                         gCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTCCGAGc   <  2:56895/149‑1 (MQ=255)
                                                                                                                                           aTTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTCCGAGcc  >  3:30869/1‑148 (MQ=255)
                                                                                                                                            ttGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTc                                                           <  8:230618/90‑1 (MQ=255)
                                                                                                                                            ttGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTc                                                           >  7:230618/1‑90 (MQ=255)
                                                                                                                                            |                                                                                                                                                     
TGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCC‑‑CGGTAAGAAAGCTCAC‑CTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTCCGAGCC  >  NC_002947/2154251‑2154537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑