Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 213,354 T→A 9.9% L6287Q (CTG→CAG)  PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947213,3540TA 9.9% 196.4 / 9.5 70L6287Q (CTG→CAG) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base T (32/31);  new base A (2/5);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

GCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGCCA  >  NC_002947/213210‑213497
                                                                                                                                                |                                                                                                                                               
gCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTc                                                                                                                                             <  7:91158/149‑1 (MQ=255)
  aCGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGt                                                                                                                                           <  8:88214/149‑1 (MQ=255)
    gTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGa                                                                                                                                         >  8:74795/1‑149 (MQ=255)
    gTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGa                                                                                                                                         >  4:7523/1‑149 (MQ=255)
     tCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGAc                                                                                                                                        >  2:200883/1‑149 (MQ=255)
     tCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCATGTCGTGag                                                                                                                                        <  8:155093/149‑2 (MQ=255)
        gTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCAGGTCGTGAgcca                                                                                                                                     <  6:252584/149‑2 (MQ=255)
         tGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCAGGTCGTGAgccaa                                                                                                                                    <  6:196339/149‑3 (MQ=255)
           aCCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGa                                                                                                                                   <  6:133133/148‑1 (MQ=255)
            ccGACGTGCTGGGAACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGc                                                                                                                                 <  7:45082/149‑1 (MQ=255)
            ccGACGATCCGGGTACGCCATGCAACGAAGGCTACTTGGTCATGGTCACCATCACGGCCGACGTGACGTCGGTGGCCGAGAACGTCAAGCCGAGGTTCAACTTGCACATCAACACCGCCTTGGCTCTCGACCTGGTCGTGTCAGTGAGc                                                                                                                                 <  4:186709/149‑1 (MQ=17)
                 gaGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGcaacaa                                                                                                                            <  1:200883/149‑1 (MQ=255)
                    ccGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGc                                                                                                                         >  2:45103/1‑149 (MQ=255)
                     cGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGcc                                                                                                                        <  1:230330/149‑1 (MQ=255)
                            cccAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGccc                                                                                                                       <  4:194831/143‑1 (MQ=255)
                            cccAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGtca                                                                                                                 >  6:12173/1‑149 (MQ=255)
                            cccAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGtca                                                                                                                 >  6:214951/1‑149 (MQ=255)
                            cccAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGCCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGccc                                                                                                                       >  3:194831/1‑143 (MQ=255)
                                   aaCGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGtcatcatcag                                                                                                          >  8:251547/1‑148 (MQ=255)
                                     cGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAgg                                                                                                        <  1:45103/149‑1 (MQ=255)
                                      gAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGc                                                                                                       <  3:146010/149‑1 (MQ=255)
                                       aaGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCt                                                                                                      >  7:130834/1‑149 (MQ=255)
                                          gCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGc                                                                                                   >  7:184886/1‑149 (MQ=255)
                                             aCTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGaa                                                                                                <  7:74795/149‑1 (MQ=255)
                                               ttGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGtcatc                                                                                                               <  8:2266/132‑1 (MQ=255)
                                                   tCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAgc                                                                                          >  8:53406/1‑149 (MQ=255)
                                                    cAAGGTCACCATAACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAgcg                                                                                         >  5:20927/1‑149 (MQ=255)
                                                     aaGGTCACCATCACGGCCGACCAGGCCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAgcgc                                                                                        >  7:78615/1‑149 (MQ=255)
                                                      aGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAgcgcg                                                                                       >  4:184137/1‑149 (MQ=255)
                                                       ggTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAgcgcgc                                                                                      >  8:151419/1‑149 (MQ=255)
                                                       ggTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAgcgcgc                                                                                      <  5:257081/149‑1 (MQ=255)
                                                        gTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCg                                                                                                                                            <  3:236715/94‑1 (MQ=255)
                                                        gTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCg                                                                                                                                            >  4:236715/1‑94 (MQ=255)
                                                           aCCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATa                                                                                  <  8:130834/149‑1 (MQ=255)
                                                            ccATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATa                                                                                  >  5:132066/1‑148 (MQ=255)
                                                                cACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACAccc                                                                             >  3:161779/1‑149 (MQ=255)
                                                                 aCGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCCGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACAccc                                                                             <  5:214951/148‑1 (MQ=255)
                                                                 aCGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACAccc                                                                             <  8:184886/148‑1 (MQ=255)
                                                                    gCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGc                                                                         >  8:68308/1‑149 (MQ=255)
                                                                        aCCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTgcg                                                                     >  6:34284/1‑149 (MQ=255)
                                                                             aCCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAgg                                                                >  7:35983/1‑149 (MQ=255)
                                                                              ccTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAgg                                                                <  3:207945/148‑1 (MQ=255)
                                                                                 cGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGAt                                                            <  7:151419/149‑1 (MQ=255)
                                                                                  ggTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGAt                                                            <  3:112794/148‑1 (MQ=255)
                                                                                      gCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGt                                                       <  3:184137/149‑1 (MQ=255)
                                                                                          agaACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTa                                                    >  1:43377/1‑148 (MQ=255)
                                                                                               gTCAAGCCGACGTTCACCGTGCTCATCAAAACCGCCTTGGCTCACGACATGGTCGTGACCCTGAGCAACAATGACCATGTCATCATCAAGGCTGGCGAAACCAGAGCGCCATAAACCCACGCTGCGCAAGGCGATGACGTCTACAACGa                                              >  5:205775/1‑149 (MQ=18)
                                                                                                          gTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAgg                                                                <  7:121411/120‑1 (MQ=255)
                                                                                                          gTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAgg                                                                >  8:121411/1‑120 (MQ=255)
                                                                                                                    cacaTCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTgg                          <  1:269618/148‑1 (MQ=255)
                                                                                                                     acaTCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGAGGTCTACAACGATGCTGGCCAGATCAGCCTggg                         >  6:267762/1‑148 (MQ=255)
                                                                                                                       aTCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCAt                      >  5:270164/1‑149 (MQ=255)
                                                                                                                        tCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATg                                            >  1:257459/1‑126 (MQ=255)
                                                                                                                        tCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATg                                            <  2:257459/126‑1 (MQ=255)
                                                                                                                        tCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTg                                         <  2:131819/129‑1 (MQ=255)
                                                                                                                        tCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTg                                         >  1:131819/1‑129 (MQ=255)
                                                                                                                          aaCACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTaa                   <  4:161779/149‑1 (MQ=255)
                                                                                                                           acacCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAAt                  <  5:267762/149‑1 (MQ=255)
                                                                                                                            cacCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAAtt                 <  8:35983/149‑1 (MQ=255)
                                                                                                                             acCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTc                <  5:12173/149‑1 (MQ=255)
                                                                                                                             acCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAACGCGATGACGTCTACAACGATGCTGCGCAGATCAGCCTGGGCATTAATTc                >  8:242760/1‑149 (MQ=255)
                                                                                                                               cGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCgg              <  3:7523/149‑1 (MQ=255)
                                                                                                                                  tcagggtCACGACCAGGTCGTGAGCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAAtt                 <  6:195661/137‑1 (MQ=255)
                                                                                                                                  tcagggtCACGACCAGGTCGTGAGCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAAtt                 >  5:195661/7‑143 (MQ=255)
                                                                                                                                   ttGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGACTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGt          >  3:148819/1‑149 (MQ=255)
                                                                                                                                     ggCTCATGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTg         >  8:92972/1‑148 (MQ=255)
                                                                                                                                      ggtCACGACCAGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGa       >  4:255836/3‑149 (MQ=255)
                                                                                                                                        tCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTGATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAg           >  5:214709/1‑143 (MQ=255)
                                                                                                                                        tCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAg           <  6:214709/143‑1 (MQ=255)
                                                                                                                                           cGACCAGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGCCa  <  3:255836/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
GCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGCCA  >  NC_002947/213210‑213497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑