| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,273,070 | C→G | 20.0% | intergenic (+35/+256) | PP_2003 → / ← PP_2004 | hypothetical protein/AraC family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,273,070 | 0 | C | G | 20.0% | 49.2 / 8.7 | 25 | intergenic (+35/+256) | PP_2003/PP_2004 | hypothetical protein/AraC family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (9/11); new base G (2/3); total (11/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01 | |||||||||||
GTGGATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGC‑‑CGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAG > NC_002947/2272928‑2273192 | gtggATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGC‑‑CGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGaaa < 4:135502/149‑1 (MQ=255) aTGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGC‑‑CGCCCCCTCCCTTTGATGGGTCGCCTGAACCGCCCCATTCGCCGGCAGCCCGCGCAAGGGc > 2:105006/1‑149 (MQ=255) cAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCTCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGGCCCTTTCGCGGGCTGCCGGCGAAAGGGTCg < 1:212771/149‑1 (MQ=38) aGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGC‑‑CGCCCCCTCCCTTTGATGGGTCGCCTGAACCGGCCCTTTCGCGGGCTGCCGGCGAAAg < 8:186278/141‑1 (MQ=255) aGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGC‑‑CGCCCCCTCCCTTTGATGGGTCGCCTGAACCGGCCCTTTCGCGGGCTGCCGGCGAAAg > 7:186278/1‑141 (MQ=255) aGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGC‑‑CGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGtt < 7:83841/149‑1 (MQ=255) ccAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGCGGCCGC‑‑CGCCCCCTCCCTTTGATGGGTCGCCTGAACCGCCCCTTTCGCCGGCAGCCCGCGCAAGGGGCGGTTcc > 3:200447/1‑148 (MQ=255) aGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGC‑‑CGACCCCTCCCTTTGATGGGTCGCCGGAACCCCCCCTTTTGCCGGCCGCCCGCGGAAGGGGCGGTTCAgg > 4:22278/1‑149 (MQ=255) gCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGC‑‑CGCCCCCTCCCTTTGATGGGTCGCCTGAACCCGCCCTTTAGCCCGCAGCCCGCGAAAGGGCCGGGTATGGCTGTaa > 1:190532/1‑148 (MQ=255) ccTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGC‑‑CGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATc < 1:105006/149‑1 (MQ=255) tgGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGC‑‑CGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATcccc < 5:100439/148‑1 (MQ=255) cTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGC‑‑CGCCCCCTCCCTTTGAAGGGTCGCCTGAACCCACCCTTTCGCCCCCACCCCCCCAAAGCGCCCGTTAAGCCTGTAAACAACCCCTGTTACAAAACCCCcgcg > 8:239093/1‑148 (MQ=255) tGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGC‑‑CGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGa > 8:235780/1‑148 (MQ=255) aCCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGC‑‑CGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGaaa > 5:177875/1‑99 (MQ=255) aCCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGC‑‑CGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGaaa < 6:177875/99‑1 (MQ=255) cccATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGC‑‑CGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCGGGCTGCCGGCGaaa < 6:149962/98‑1 (MQ=255) cccATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGC‑‑CGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCGGGCTGCCGGCGaaa > 5:149962/1‑98 (MQ=255) ccATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGC‑‑CGCCCCCTCCCTTTGATGGGTCGCCATAACCCACCCAATACCCCGCACCCCGCCAAAAGGCCGGTTAAGGCTGTAAACATACCCAGTTACAAAACCCCCCCGATAAcat > 6:174857/1‑147 (MQ=255) gTGCAGCAGGATGCCCAGCCGGCCGAGGCCGC‑‑CGCCCCCGCCCTTTGATGGGTCGCCTGAACCGCCCCATTGGCCGGCCGCCCGCGCAAGGGCCGGGTCAGGCTGGAAACAACCCCGGGTACAGACGCCCCGCGCGAACtttgagacaa > 5:103666/1‑142 (MQ=255) cagcagGATGCCCAGCCGGCCGAGGCCGC‑‑CGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCt < 7:186097/149‑1 (MQ=255) ggATGCCCAGCCGGCCGAGGCCGC‑‑CGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTAc < 7:235780/149‑1 (MQ=255) ccggccgAGGCCGC‑‑CGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACt < 5:265059/149‑1 (MQ=255) aGGCCGC‑‑CGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGc < 2:213298/149‑1 (MQ=255) tCGCCTGAAACGACCCTTTCGCCTGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCTATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGc < 7:239093/149‑1 (MQ=255) cTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAg < 4:200447/149‑1 (MQ=255) | GTGGATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGC‑‑CGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAG > NC_002947/2272928‑2273192 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |