Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 225,366 C→T 9.0% A99T (GCC→ACC)  PP_0173 ← transcriptional factor‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947225,3660CT 9.0% 186.5 / 4.1 66A99T (GCC→ACC) PP_0173transcriptional factor‑like protein
Reads supporting (aligned to +/- strand):  ref base C (26/33);  new base T (2/4);  total (29/37)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGCATCCCGCAACTGCCGCGCCAGTGCATCGCTGATTCGCGCCACCAGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAGGTCACGCTCGACAGTGCGTTTGCTGACCGTGAAGCCAACGTCACGCAGGCGCCAGACCAGCTCGGCGCTG  >  NC_002947/225225‑225508
                                                                                                                                             |                                                                                                                                              
gcgcATCCCGCAACTGCCGCGCCAGTGCATCGCTGATTCGCGCCACCAGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAg                                                                                                                                         >  4:241422/1‑149 (MQ=255)
 cgcATCCCGCAACTGCCGCGCCAGTGCATCGCTGATTCGCGCCACCAGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGt                                                                                                                                        <  6:191844/149‑1 (MQ=255)
 cgcATCCCGCAACTGCCGCGCCAGTGCATCGCTGATTCGCGCCACCAGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGt                                                                                                                                        <  3:57760/149‑1 (MQ=255)
 cgcATCCCGCAACTGCCGCGCCAGTGCATCGCTGATTCGCGCCACCAGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGt                                                                                                                                        <  4:17464/149‑1 (MQ=255)
  gcATCCCGCAACTGCCGCGCCAGTGCATCGCTGATTCGCGCCACCAGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCATTGGCCGACCAGTg                                                                                                                                       >  7:190963/1‑149 (MQ=255)
        cGCAACTGCCGCGCCAGTGCATCGCTGATTCGCGCCACCAGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGTCGGCCAGTGccgtgg                                                                                                                                 <  1:114734/149‑5 (MQ=255)
        cGCAACTGCCGCGCCAGTGCATCGCTGATTCGCGCCACCAGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGTCGGCCAGTGccgtgg                                                                                                                                 <  2:192483/149‑5 (MQ=255)
        cGCAACTGCCGCGCCAGTGCATCGCTGATTCGCGCCACCAGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAAcc                                                                                                                                 >  8:242126/1‑149 (MQ=255)
         gCAACTGCCGCGCCAGTGCATCGCTGATTCGCGCCACCAGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCg                                                                                                                                <  5:18314/149‑1 (MQ=255)
                ccGCGCCAGTGCATCGCTGATTCGCGCCACCAGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCgaatggaa                                                                                                                         <  7:177713/149‑1 (MQ=255)
                     ccAGTGCATCGCTGATTCGCGCCACCAGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGctc                                                                                                                    <  7:63692/149‑1 (MQ=255)
                      cAGTGCATCGCTGATTCGCGCCACCAGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGTAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGTCGGCCAGTGcc                                                                                                                                     <  1:63762/131‑1 (MQ=255)
                      cAGTGCATCGCTGATTCGCGCCACCAGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGTAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGTCGGCCAGTGcc                                                                                                                                     >  2:63762/1‑131 (MQ=255)
                      cAGTGCATCGCTGATTCGCGCCACCAGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGctct                                                                                                                   <  4:22588/149‑1 (MQ=255)
                        gTGCATCGCTGATTCGCGCCACCAGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCgaatggaa                                                                                                                         >  2:264436/1‑141 (MQ=255)
                        gTGCATCGCTGATTCGCGCCACCAGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCgaatggaa                                                                                                                         <  1:264436/141‑1 (MQ=255)
                             tCGCTGATTCGCGCCACCAGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGt                                                                                                                                        <  6:96127/121‑1 (MQ=255)
                             tCGCTGATTCGCGCCACCAGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGt                                                                                                                                        >  5:96127/1‑121 (MQ=255)
                               gCTGATTCGCGCCACCAGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTg                                                                                                           <  7:122841/148‑1 (MQ=255)
                                cTGATTCGCGACACCAGCTCCACGCCCTCACCCTGCGAAGGCTGCAACGCATCCCCTAGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACTGCACTGGACGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCg                                                                                                         >  1:76434/1‑149 (MQ=255)
                                   aTTCGCGCCACCAGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGtt                                                                                                       <  7:242126/148‑1 (MQ=255)
                                   aTTCGCGCCACCAGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATGGCggag                                                                                                      >  1:96668/1‑146 (MQ=255)
                                      cgcgCCACCAGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCa                                                                                                    <  4:215362/148‑1 (MQ=255)
                                       gcgcCACCAGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGc                                                                                                  >  4:4613/1‑149 (MQ=255)
                                          ccaccaGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGt                                                                                                        >  4:100948/1‑140 (MQ=255)
                                          ccaccaGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGt                                                                                                        <  3:100948/140‑1 (MQ=255)
                                          ccaccaGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCgg                                                                                                >  6:3142/1‑148 (MQ=255)
                                            accaGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGaa                                                                                             >  3:213785/1‑149 (MQ=255)
                                             ccaGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGa                                                                                                                                             >  5:87497/1‑100 (MQ=255)
                                             ccaGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGa                                                                                                                                             <  6:87497/100‑1 (MQ=255)
                                             ccaGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAat                                                                                            <  3:4613/149‑1 (MQ=255)
                                             ccaGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGAGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGaa                                                                                             >  4:228786/1‑148 (MQ=255)
                                                  tCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAg                                                                                       >  4:238596/1‑149 (MQ=255)
                                                    ctctcccTCACCCTGCGATGGCTGCTACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGACCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGTGAATATCa                                                                                        <  2:76434/142‑1 (MQ=255)
                                                    cACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGc                                                                                      >  7:38527/1‑148 (MQ=255)
                                                             aCCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGt                                                                            <  3:238596/149‑1 (MQ=255)
                                                                   cGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAg                                                                        <  5:3142/147‑1 (MQ=255)
                                                                          tGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAgg                                                                       >  6:78353/1‑141 (MQ=255)
                                                                          tGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAgg                                                                       <  5:78353/141‑1 (MQ=255)
                                                                            cAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAg                                                                                                                                         <  4:21523/73‑1 (MQ=255)
                                                                            cAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAg                                                                                                                                         >  3:21523/1‑73 (MQ=255)
                                                                            cAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAGGTCACGCTCGa                                                             >  4:167358/1‑149 (MQ=255)
                                                                                  aTCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAGGTCACGCTCGACAGTGc                                                       >  1:114304/1‑149 (MQ=255)
                                                                                    ccccTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAGGTCACGCTCGACAGTGCGt                                                     >  3:105448/1‑149 (MQ=255)
                                                                                     cccTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAGGTCACGCTCGACAGTGCGtt                                                    <  3:241422/149‑1 (MQ=255)
                                                                                      ccTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATAGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAGGTCACGCTCGACAGTGCGttt                                                   <  5:18742/149‑1 (MQ=255)
                                                                                       atCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAGGTCACGCTCGACAGTGCGTTTg                                                  <  3:183599/148‑1 (MQ=255)
                                                                                          gCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAGGTCACGCTCGACAGTGCGTTTGc                                                 <  2:114304/147‑1 (MQ=255)
                                                                                               tgtgTCAGCAAGTCGAAATTACCGTGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAGGTCACGCTCGACAGTGCGTTTGCTGACCGt                                          <  3:228786/149‑1 (MQ=255)
                                                                                                 tgtCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAGGTCACGCTCGACAGTGCGTTTGCTGACCGTg                                         <  7:17308/148‑1 (MQ=255)
                                                                                                   tCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAGGTCACGCTCGACAGTGCGTTTGCTGACCGTGaa                                       <  2:269293/148‑1 (MQ=255)
                                                                                                   tCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAGGTCACGCTCGACAGTGCGTTTGCTGACCGTGaa                                       <  4:105448/148‑1 (MQ=255)
                                                                                                      gCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAGGTCACGCTCGACAGTGCGTTTGCTGACCGTGa                                        >  5:184820/1‑144 (MQ=255)
                                                                                                      gCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAGGTCACGCTCGACAGTGCGTTTGCTGACCGTGa                                        <  6:184820/144‑1 (MQ=255)
                                                                                                       cAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAACCTCGTTGAGGTCACGCTCGACAGTGCCTTTGCTGACCGTGAAGCCaa                                  >  7:185966/1‑149 (MQ=255)
                                                                                                          gTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAGGTCACGCTCGACAGTGCGTTTGCTGACCGTGaa                                       >  3:173814/1‑141 (MQ=255)
                                                                                                          gTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAGGTCACGCTCGACAGTGCGTTTGCTGACCGTGaa                                       <  4:173814/141‑1 (MQ=255)
                                                                                                                              cGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAGGTCACGCTCGACAGTGCGTTTGCTGACCGTGAAGCCAACGTCACGCAGGCGCCAGACCAg            <  4:213785/148‑1 (MQ=255)
                                                                                                                               gCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAGGTCACGCTCGACAGTGCGTTTGCTGACCGTGAAGCCAACGTCACGCAGGCGCCAGACCAGCt          >  1:134487/1‑149 (MQ=255)
                                                                                                                               gCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAGGTCACGCTCGACAGCGCGTTTGCTGACCGTGAAGCCAACGTCACGCAGGCGCCAGACCAGCt          >  5:63066/1‑149 (MQ=255)
                                                                                                                                cccACGGCACTGGTCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAGGTCACGCTCGACAGTGCGTTTGCTGACCg                                           >  2:57318/1‑115 (MQ=255)
                                                                                                                                cccACGGCACTGGTCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAGGTCACGCTCGACAGTGCGTTTGCTGACCg                                           <  1:57318/115‑1 (MQ=255)
                                                                                                                                   aCGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAGGTCACGCTCGACAGTGCGTTTGCTGACCGTGAAGCCAACGTCACGCAGGCGCCAGACCAGCTCGgc      >  6:9874/1‑149 (MQ=255)
                                                                                                                                       cACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAGGTCACGCTCGACAGTGCGTTTGCTGACCGTGAAGCCAACGTCACGCAGGCGCCAGACCAGCTCGgcg     >  7:45396/1‑146 (MQ=255)
                                                                                                                                       cACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAGGTCACGCTCGACAGTGCGTTTGCTGACCGTGAAGCCAACGTCACGCAGGCGCCAGACCAGCTCGgcg     <  8:45396/146‑1 (MQ=255)
                                                                                                                                       cACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAGGTCACGCTCGACAGTGCGTTTGCTGACCGTGAAGCCAACGTCACGCAGGCGCCAGACCAGCTCGGCGCTg  <  2:96668/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                              
GCGCATCCCGCAACTGCCGCGCCAGTGCATCGCTGATTCGCGCCACCAGCTCCACGCCCTCACCCTGCGATGGCTGCAACGCATCCCCTCGCAGATGTGTCAGCAAGTCGAAATTACCGCGCAATTCGCCCACGGCACTGGCCGACCAGTGCCAACCGAATGGAATGCTCTTGTCATTGCGTTCCAGCGGGAATATCAGCGAAAGCTCGTTGAGGTCACGCTCGACAGTGCGTTTGCTGACCGTGAAGCCAACGTCACGCAGGCGCCAGACCAGCTCGGCGCTG  >  NC_002947/225225‑225508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑