Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,289,100	C→T	8.5%	D461D (GAC→GAT)	pepN →	aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,289,100	0	C	T	8.5%	130.3 / 2.6	47	D461D (GAC→GAT)	pepN	aminopeptidase N
Reads supporting (aligned to +/- strand):  ref base C (22/21);  new base T (2/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.19e-01

TTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGACCCCGGACAAGGCCGAGAAACTGCCGTTCGTGATCCCGGTGGAGCTGGGCCTGCTGGATGCCGACGGCAACGACCTGCCGCTGCAACTGACTGGC  >  NC_002947/2288957‑2289244                                                                                                                                                 |                                                                                                                                                 tttGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGAcgccgc                                                                                                                                             >  8:206815/1‑149 (MQ=255)   tGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGAcgccgccg                                                                                                                                           >  6:204384/1‑149 (MQ=255)          cATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGAc                                                                                                                                    >  5:84874/1‑149 (MQ=255)          cATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGCTCAGCGAGGCGTATGACGCCGCCGCGCAGAc                                                                                                                                    >  5:154748/1‑149 (MQ=255)              atgGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGCGGTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCCCAGACCTa                                                                                                                                 >  1:209256/1‑149 (MQ=255)              atgGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTAc                                                                                                                                <  4:183920/149‑1 (MQ=255)               tgGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACa                                                                                                                               <  7:206815/149‑1 (MQ=255)                         gTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGtt                                                                                                                     >  7:112773/1‑149 (MQ=255)                              caccGACGACTTCATCAAGGCCATGGAAGACGCCAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTcc                                                                                                                   <  6:44236/146‑1 (MQ=255)                              caccGACGACTTCATCAAGGCCATGGAAGACGCCAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTcc                                                                                                                   >  5:44236/1‑146 (MQ=255)                                 cgacgaCTTCATCAAGGCCATGGAAGACGCCAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCaga                                                                                                             <  2:209256/149‑1 (MQ=255)                                  gacgacTTCATCAAGGCCATGGAAGACGCCAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCagag                                                                                                            >  1:160232/1‑149 (MQ=255)                                     gacTTCATCAAGGCCATGGAAGACGCCAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGATGCCGCCGCGCAGACCTACAGCCTGa                                                                                                                         >  8:8633/1‑133 (MQ=255)                                     gacTTCATCAAGGCCATGGAAGACGCCAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGATGCCGCCGCGCAGACCTACAGCCTGa                                                                                                                         <  7:8633/133‑1 (MQ=255)                                     gacTTCATCAAGGCCATGGAAGACGCCAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGATGCCGCCGCGCAGACCTACAGCCTGa                                                                                                                         <  1:90590/133‑1 (MQ=255)                                     gacTTCATCAAGGCCATGGAAGACGCCAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGATGCCGCCGCGCAGACCTACAGCCTGa                                                                                                                         >  2:90590/1‑133 (MQ=255)                                        ttCATCAAGGCCATGGAAGACGCCAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGccc                                                                                                      >  2:44460/1‑149 (MQ=255)                                             caAGGCCATGGAAGACGCCAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGAcgcc                                                                                                                                               >  8:150595/1‑103 (MQ=255)                                             caAGGCCATGGAAGACGCCAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGAcgcc                                                                                                                                               <  7:150595/103‑1 (MQ=255)                                                   cATGGAAGACGCCAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGCCCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGACCCCg                                                                                            >  7:123704/1‑148 (MQ=255)                                                    aTGGAAGACGCCAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGACCCCGGa                                                                                          >  3:214344/1‑149 (MQ=255)                                                    aTGGAAGACGCCAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGACCCCGGa                                                                                          <  6:194785/149‑1 (MQ=255)                                                         aGACGCCAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGACCCCGGACAAgg                                                                                     >  6:259405/1‑149 (MQ=255)                                                         aGACGCCAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGACCCCGGACAAgg                                                                                     <  4:214344/149‑1 (MQ=255)                                                          gACGCCAACGGC‑GTCGACTATACCCAGTTCAAGCGCTGGAACAGCCAGGCGGGCACCCCACGCCTGGACCTCAGCCAGGCGTATCACCCCGCCGCGCAGACCTACAGCCAGACGATCCGCCAGAGCTGCCCGCAGACCCCGGACAAgcc                                                                                    >  6:270274/1‑147 (MQ=255)                                                            cGCCAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGACCCCGGACAAGGcc                                                                                   <  6:154748/148‑1 (MQ=255)                                                               cAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGCGCTGCCCGCAGACCCCGGACAAgagtcgg                                                                                >  5:126584/1‑142 (MQ=255)                                                                  cGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGACCCCGGACAAGGCCGAGAAAc                                                                            <  8:112773/149‑1 (MQ=255)                                                                             tttACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGACCCCGGACAAGGCCGAGAAACTGCCGTTCGt                                                                  >  3:126370/1‑149 (MQ=255)                                                                                  ccAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGa                                                                                                 >  6:155571/1‑113 (MQ=255)                                                                                  ccAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGa                                                                                                 <  5:155571/113‑1 (MQ=255)                                                                                     gTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGACCCCGGACAAGGCCGAGAAACTGCCGTTCGTGATCCCgg                                                          >  3:212439/1‑149 (MQ=255)                                                                                           gcgcTGGTACAGCGAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCg                                                                                                                  <  6:186292/87‑1 (MQ=255)                                                                                           gcgcTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCg                                                                                                                  >  5:186292/1‑87 (MQ=255)                                                                                           gcgcTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGACCCCGGACAAGGCCGAGAAACTGCCGTTCGTGATCCCGGTGGAGc                                                    >  8:114463/1‑149 (MQ=255)                                                                                           gcgcTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGACCCCGGACAAGGCCGAGAAACTGCCGTTCGTGATCCCGGTGGAGc                                                    >  8:110988/1‑149 (MQ=255)                                                                                            cgcTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGACCCCGGACAAGGCCGAGAAACTGCCGTTCGTGATCCCGGTGGAGCt                                                   <  4:126370/149‑1 (MQ=255)                                                                                                    cAGCCAGGGGGGGACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGGTCCGGCAGAGCTGCCCGCAGACCCCGGACATGCCCGAGAAACTGCCGTTCGTGATCCCGGCGGAGCTGGGCctgc                                           <  5:270274/149‑1 (MQ=255)                                                                                                       ccAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGACCCCGGACAAGGCCGAGAAACTGCCGTTCGTGATCCCGGTGGAGCTGGGCctgctg                                         >  1:268185/1‑148 (MQ=255)                                                                                                              ggCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGACCCCGGACAAGGCCGAGAAACTGCCGTTCGTGATCCCGGTGGAGCTGGGCCTGCTGGATGCCGa                                 <  7:114463/149‑1 (MQ=255)                                                                                                              ggCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGACCCCGGACAAGGCCGAGAAACTGCCGTTCGTGATCCCGGTGGAGCTGGGCCTGCTGGATGCCGa                                 <  7:110988/149‑1 (MQ=255)                                                                                                                cACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGACCCCGGACAAGGCCGAGAAACTGCCGTTCGTGATCCCGGTGGAGCTGGGCCTGCTGGATGCCGACg                               <  6:181467/149‑1 (MQ=255)                                                                                                                cACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGACCCCGGACAAGGCCGAGAAACTGCCGTTCGTGATCCCGGTGGAGCTGGGCCTGCTGGATGCCGACg                               <  5:204384/149‑1 (MQ=255)                                                                                                                    ccACGCCTGGTTGGCAGGGAGGCGTATGACGCCGGCGCGCAGACCTACAGCCTTCCTTTCCGCCAGAGCTGCCCGCCGACCCCGGACACGGCCGAGAAACTGCCGTTCGTGATCCCTGTGGAGCTGGGCCTGCTGGATGCCGACgg                              <  8:229521/146‑1 (MQ=255)                                                                                                                    ccACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGACCCCGGACAAGGCCGAGAAACTGCCGTTCGTGATCCCGGTGGAGCTGGGCCTGCTGGATGCCGACgg                              >  7:229521/1‑146 (MQ=255)                                                                                                                    ccACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGACCCCGGACAAGGCCGAGAAACTGCCGTTCGTGATCCCGGTGGAGCTGGGCCTGCTGGATGCCGACGGCaa                           <  4:212439/149‑1 (MQ=255)                                                                                                                          cTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGACCCCGGACAAGGCCGAGAAACTGCCGTTCGTGATCCCGGTGGAGCTGGGCCTGCTGGATGCCGACGGCAACGACCt                     <  1:247580/149‑1 (MQ=255)                                                                                                                                         gCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGACCCCGGACAAGGCCGAGAAACTGCCGTTCGTGATCCCGGTGGAGCTGGGCCTGCTGGATGCCGACGGCAACGACCTGCCGCTGCAactgac      <  2:268185/149‑1 (MQ=255)                                                                                                                                           gTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGACCCCGGACAAGGCCGAGAAACTGCCGTTCGTGATCCCGGTGGAGCTGGGCCTGCTGGATGCCGACGGCAACGACCTGCCGCTGCAactgactg    >  6:61639/1‑149 (MQ=255)                                                                                                                                           gTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGACCCCGGACAAGGCCGAGAAACTGCCGTTCGTGATCCCGGTGGAGCTGGGCCTGCTGGATGCCGACGGCAACGACCTGCCGCTGCAactgactg    >  2:121157/1‑149 (MQ=255)                                                                                                                                              tGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGACCCCGGACAAGGCCGAGAAACTGCCGTTCGTGATCCCGGTGGAGCTGGGCCTGCTGGATGCCGACGGCAACGACCTGCCGCTGCAACTGACTGGc  <  1:121157/148‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                 TTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGC‑GTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCACCCCACGCCTGGAGGTCAGCGAGGCGTATGACGCCGCCGCGCAGACCTACAGCCTGACGTTCCGCCAGAGCTGCCCGCAGACCCCGGACAAGGCCGAGAAACTGCCGTTCGTGATCCCGGTGGAGCTGGGCCTGCTGGATGCCGACGGCAACGACCTGCCGCTGCAACTGACTGGC  >  NC_002947/2288957‑2289244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑