| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,301,427 | G→C | 8.3% | intergenic (‑71/‑260) | sbcD ← / → PP_5497 | exonuclease subunit SbcD/rhs repeat‑associated core domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,301,427 | 0 | G | C | 8.3% | 141.6 / 4.7 | 48 | intergenic (‑71/‑260) | sbcD/PP_5497 | exonuclease subunit SbcD/rhs repeat‑associated core domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base G (20/24); new base C (2/2); total (22/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01 | |||||||||||
CGTGTTCGAAGTCGCGTTCCTGGCCGTGCAGGCTTTGGCCCAGGTGCCAGTCGGAGGTATGAAACAGACGCATGGATGACCTGTTGGTGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTCTCATAGAACTGCGCATCACTTACTGATTTAGCAGGGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAATCCAACGCGGTGCATGGCACCGGCTGCGCCGGTGTTCGCGGGC > NC_002947/2301284‑2301565 | cGTGTTCGAAGTCGCGTTCCTGGCCGTGCAGGCTTTGGCCCAGGTGCCAGTCGGAGGTATGAAACAGACGCATGGATGACCTGTTGGTGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCgg > 7:122651/1‑149 (MQ=255)cGTGTTCGAAGTCGCGTTCCTGGCCGTGCAGGCTTTGGCCCAGGTGCCAGTCGGAGGTATGAAACAGACGCATGGATGACCTGTTGGTGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCgg > 8:238219/1‑149 (MQ=255) ttCGAAGTCGCGTTCCTGGCCGTGCAGGCTTTGGCCCAGGTGCCAGTCGGAGGTATGAAACAGACGCATGGATGACCTGTTGGTGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCACAGCGGcccc < 8:161534/149‑2 (MQ=255) gcgTTCCTGGCCGTGCAGGCTTGAGCCCAGGTGCCAGTCGGAGGTATGAAACAGACGCATGGATGACCTGTTGGTGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCggg > 7:40236/1‑148 (MQ=255) ccTGGCCGTGCAGGCTTTGGCCCAGGTGCCAGTCGGAGGTATGAAACAGACGCATGGATGACCTGTTGGTGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGc < 8:122651/148‑1 (MQ=255) ctatctGCAGGCTTTGGCCCAGGGGCCAGGCGGAGGGATGAATCAGACGCATGGATGACCTGTTGGTTGTGAGGCGGGCAAGCGTGGAAAGGGCGGTATGGTCACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGcctg < 7:172875/143‑3 (MQ=255) gCCGTGCAGGCTTTGGCCCAGGTGCCAGTCGGAGGTATGAAACAGACGCATGGATGACCTGTTGGTGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCg < 8:216980/126‑1 (MQ=255) gCCGTGCAGGCTTTGGCCCAGGTGCCAGTCGGAGGTATGAAACAGACGCATGGATGACCTGTTGGTGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCg > 7:216980/1‑126 (MQ=255) ccGTGCAGGCTTTGGCCCAGGTGCCAGTCGGAGGTATGAAACAGACGCATGGATGACCTGTTGGTGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTt < 4:180267‑M1/149‑2 (MQ=255) ccGTGCAGGCTTTGGCCCAGGTGCCAGTCGGAGGTATGAAACAGACGCATGGATGACCTGTTGGTGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTt < 7:82523‑M1/149‑2 (MQ=255) gTGCAGGCTTTGGCCCAGGTGCCAGTCGGAGGTATGAAACAGACGCATGGATGACCTGTTGGTGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtc > 7:26932‑M1/1‑146 (MQ=255) cAGGCTTTGGCCCAGGTGCCAGTCGGAGGTTTGAAACAGACGCATGGATGACCTGTTGGTGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctatc < 8:96960‑M1/149‑7 (MQ=255) cAGGCTTTGGCCCAGGTGCCAGTCGGAGGTAAGAAACAGACGCATGGATGACCTGTTGGAGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGGAACCCAAGCGTGGGGCCGCTGGGCGGCCCGAGCGCGCGccagccag > 8:172875/1‑141 (MQ=255) aagGATTTGGCCCATGTGTCAGTCGGAGGTATGAAACAGACGCATGGATGAGCTGTTGGTGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctat < 4:142006‑M1/147‑6 (MQ=255) ccAGGTGCCAGTCGGAGGTATGAAACAGACGCATGGATGACCTGTTGGTGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaaga > 3:11003‑M1/1‑132 (MQ=255) ccAGTCGGAGGTATGAAACAGACGCATGGATGACCTGTTGGTGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataaccca < 5:232894‑M1/148‑24 (MQ=255) aGTCGGAGGTATGAAACAGACGCATGGATGACCTGTTGGTGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactg > 5:136585‑M1/1‑123 (MQ=255) tCGGAGGTATGAAACAGACGCATGGATGACCTGTTGGTGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATTGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgtt < 1:188521‑M1/149‑29 (MQ=255) cGGAGGTATGAAACAGACGCATGGATGGCCTGTTGGTGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGAGTGGGGCCGCTGTGCTGCCCTATCGCTGGCAAGCCAGATtctatcaaacaaaagctaaaccactctt > 4:215381‑M1/1‑120 (MQ=255) ggAGGTATGAAACAGACGCATGGATGACCTGTTGGTGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgttct > 2:154973‑M1/1‑119 (MQ=255) tATGAAACAGACGCATGGATGACCTGTTGGTGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgttctttcta < 7:238219‑M1/149‑36 (MQ=255) aTGAAACAGACGCATGGATGACCTGTTGGTGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgttctttctag > 8:171437‑M1/1‑113 (MQ=255) tGAAACAGACGCATGGATGACCTGTTGGTGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgttctttctag < 8:26932‑M1/148‑37 (MQ=255) gCATGGATGACCTGTTGGTGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgttctttctagcgaaaaaaa > 3:7888‑M1/1‑102 (MQ=255) cttggtcgacctgttGGTGTCGAGTGGGGCAAGCGGGGAAATGGCGGTATGGTATCCCAAGGGTGGGGCCGCTGTGCGGCCCGATCGCGTGCAAGCCAGCTtctatcaaacaaaagataacgcactgttctttctagcgaaaaaaacag < 7:42664‑M1/142‑49 (MQ=255) ggATGACCTGTTGGTGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgttctttctagcgaaaaaaacaggcg > 6:84255‑M1/1‑98 (MQ=255) ttGGTGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgttctttctagcgaaaaaaacaggcgcgcggggt < 2:241008‑M1/147‑60 (MQ=255) tgtCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgttctttctagcgaaaaaaacaggcgcgcggggtcaatgg > 5:224249‑M1/1‑84 (MQ=255) tgtCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgttctttctagcgaaaaaaacaggcgcgcggggtcaatgg < 6:136585‑M1/149‑66 (MQ=255) tctggcaaGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgttctttctagcgaaaaaaacaggcgcgcggggtcaatggcggcgaa < 4:7888‑M1/146‑73 (MQ=255) catgcggGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgttctttctagcgaaaaaaacaggcgcgcgggggcaatggcggcgaagtgtt < 3:215381‑M1/146‑78 (MQ=255) aaGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgttctttctagcgaaaaaaacaggcgcgcggggtcaatggcggcgaagtgttc < 8:40236‑M1/149‑79 (MQ=255) cGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgttctttctagcgaaaaaaacaggcgcgcggggtcaatggcggcgaagtgctcact < 7:171437‑M1/149‑82 (MQ=255) ggggAAAGGGCGGTATGGTAACCCAAGCGCGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgttctttctagcgaaaaaaacaggcgcgcggtttcaatggctgcgaagtgttcactc < 4:106210‑M1/149‑83 (MQ=255) gCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgttctttgtagcgaaaaaaacaggcgcgcggggtcaatggcggcgaagtggtcactcttgtatacg > 5:38234‑M1/1‑58 (MQ=255) gCGGTATGGTAACCCAAGCGTGGGGCCGCTGAGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgttctttctagcgaaaaaaacaggcgcgcggggtcaatggcggcgaagtgttcactcttctatacg > 1:27762‑M1/1‑58 (MQ=255) ggTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgttctttctagcgaaaaaaacaggcgcgcggggtcaatggcggcgaagtgttcactcttctatacgtg < 6:39821‑M1/149‑94 (MQ=255) gTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgttctttctagcgaaaaaaaca > 3:95182‑M1/1‑55 (MQ=255) gTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgttctttctagcgaaaaaaaca < 4:95182‑M1/102‑48 (MQ=255) gTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgttctttctagcgaaaaaaacaggcgcgcggggtcaatggcggcgaagtgttcactcttctatacgtggtcac < 4:109862‑M1/148‑99 (MQ=255) gTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgtactttctagcgaaaaaaacaggcgcgcggggtcaatggcggcgaagtgttcactcttctatacgtggtca > 1:84783‑M1/1‑50 (MQ=255) aCCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtcaatcaaacaaaagataacccacatttctttctagcgaaaaaaaaaggcgcgcggggtcaatggcggcgaagtgttcactcttctattcttggtcacaga > 8:193995‑M1/1‑47 (MQ=255) cccATGCGTGGGGCCGCTGTGCGTCCCGATCGCGGGCAAGCAAGCTtctatcaaacaaaagataacccactgttctttctagcgaataaaacaggcgcgcggggtcaatggaggcgaagtgttcactcttctatacgtggtgacagcaa < 5:19796‑M1/149‑104 (MQ=255) ccAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgttctttctagcgaaaaaaacaggcgcgcggggtcaatggcggcgaagtgttcactcttctatacgtggtcacagcaag > 3:185623‑M1/1‑45 (MQ=255) gatcgggccgcACAGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgttctttctagcgaaaaaaacaggcgcgcggggtcaatggcggcgaagtgttcactcttctatacgtggtcacagcaagctgtg > 8:111066‑M1/5‑40 (MQ=255) gTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgttctttctagcgaaaaaaacaggcgcgcggggtcaatggcggcgaagtgttcactcttctatacgtggtcacagcaagctgtgc < 1:133788‑M1/149‑111 (MQ=255) atcgggccgCACAGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgttctttctagcgaaaaaaacaggcgcgcggggtcaatggcggcgaagtgttcactcttctatacgtggtcacagcaagctgtgc < 7:111066‑M1/146‑111 (MQ=255) tcgggccGCACAGCGGCCCGATCGCGGGCAAGCCAGCTtctatcaaacaaaagataacccactgttctttctagcgaaaaaaacaggcgcgcggggtcaatggcggcgaagtgttcactcttctatacgtggtcacagcaagctgtgcc > 6:240686‑M1/3‑38 (MQ=255) | CGTGTTCGAAGTCGCGTTCCTGGCCGTGCAGGCTTTGGCCCAGGTGCCAGTCGGAGGTATGAAACAGACGCATGGATGACCTGTTGGTGTCGAGTCGGGCAAGCGGGGAAAGGGCGGTATGGTAACCCAAGCGTGGGGCCGCTGTGCGGCCCGATCGCGGGCAAGCCAGCTCTCATAGAACTGCGCATCACTTACTGATTTAGCAGGGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAATCCAACGCGGTGCATGGCACCGGCTGCGCCGGTGTTCGCGGGC > NC_002947/2301284‑2301565 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |