| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,361,724 | C→G | 15.0% | R718P (CGG→CCG) | PP_2076 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,361,724 | 0 | C | G | 15.0% | 102.2 / 10.1 | 40 | R718P (CGG→CCG) | PP_2076 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (20/14); new base G (3/3); total (23/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
CTTTTGTAATGCTCAATCGAGTCAGTGAAATCCCACCACGCGAGTACCAACCCCCCGGTGCCAGCTAAACTGGCGCCCCATAGTTTCAAACGCCCTAGAGCGGCCGCTGCTCCTCGTCCGGTCACACTGTTCGCGCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCTTCACCCGCGCATCACTGTCAGG‑CAGTTCGCGCGCTTTGAAGGCCATCAGCATCC > NC_002947/2361583‑2361862 | ctTTTGTAATGCTCAATCGAGTCAGTGAAATCCCACCACGCGAGTACCAACCCCCCGGTGCCAGCTAAACTGGCGCCCCATAGTTTCAAACGCCCTAGAGCGGCCGCTGCTCCTCGTCCGGTCACACTGTTCGCGCGGTACGGCGCGaa < 1:260780/149‑2 (MQ=255) aaTCGAGTCAGTGAAATCCCACCACGCGAGTACCAACCCCCCGGTGCCAGCTAAACTGGCGCCCCATAGTTTCAAACGCCCTAGAGCGGCCGCTGCTCCTCGTCCGGTCACACTGTTCGCGCCGTACCGCGCGACCACCTGGTCcaca > 4:182590/1‑148 (MQ=255) accacGCGAGTACCAACCCCCCGGTGCCAGCTAAACTGGCGCCCCATAGTTTCAAACGCCCTAGAGCGGCCGCTGCTCCTCGTCCGGTCACACTGTTCGCGCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAg > 3:3190/1‑149 (MQ=255) cAACCCCCCGGTGCCAGCTAAACTGGCGCCCCATAGTTTCAAACGCCCTAGAGCGGCCGCTGCTCCTCGTCCGGTCACACTGTTCGCGCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCg > 8:201815/1‑149 (MQ=255) ccccGGTGCCAGCTAAACTGGCGCCCCATAGTTTCAAACGCCCTAGAGCGGCCGCTGCTCCTCGTCCGGTCACACTGTTCGCGCCGTACCGCGCGac > 1:246709/1‑97 (MQ=255) ccccGGTGCCAGCTAAACTGGCGCCCCATAGTTTCAAACGCCCTAGAGCGGCCGCTGCTCCTCGTCCGGTCACACTGTTCGCGCCGTACCGCGCGac < 2:246709/97‑1 (MQ=255) aaaCTGGCGCCCCATAGTTTCAAACGCCCTAGAGCGGCCGCTGCTCCTCGTCCGGTCACACTGTTCGCGCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAg < 4:178807/127‑1 (MQ=38) aaaCTGGCGCCCCATAGTTTCAAACGCCCTAGAGCGGCCGCTGCTCCTCGTCCGGTCACACTGTTCGCGCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAg > 3:178807/1‑127 (MQ=38) aaCTGGCGCCCCATAGTTTCAAACGCCCTAGAGCGGCCGCTGCTCCTCGTCCGGTCACACTGTTCGCGCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCa < 3:182590/147‑1 (MQ=37) aCTGGCGCCCCATAGTTTCAAACGCCCTAGAGCGGCCGCTGCTCCTCGTCCGGTCACACTGTTCGCGCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGa < 7:201815/149‑1 (MQ=38) cATAGTTTCAAACGCCCTAGAGCGGCCGCTGCTCCTCGTCCGGTCACACTGTTCGCGCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCtt > 5:19722/1‑149 (MQ=39) aaCGCCCTAGAGCGGCCGCTGCTCCTCGTCCGGTCACACTGTTCGCGCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGa < 7:185773/129‑1 (MQ=40) aaCGCCCTAGAGCGGCCGCTGCTCCTCGTCCGGTCACACTGTTCGCGCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGa > 8:185773/1‑129 (MQ=40) aCGCCCTAGAGCGGCCGCTGCTCCTCGTCCGGTCACACTGTTCGCGCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCTTCACCCGCGCAt < 6:19722/149‑1 (MQ=39) cGCCCTAGAGCGGCCGCTGCTCCTCGTCCGGTCACACTGTTCGCGCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCTTCACCCGCGCATc > 5:9833/1‑149 (MQ=39) cccTAGAGCGGCCGCTGCTCCTCGTCCGGTCACACTGTTCGCGCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCTTCACCCGCGCATCAc > 3:262694/1‑149 (MQ=39) gagCGGCCGCTGCTCCTCGTCCGGTCACACTGTTCGCGCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCTTCACCCGCGCATCACTGTCa < 4:3190/149‑1 (MQ=38) ctgctCCTCGTCCGGTCACACTGTTCGCGCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCTTCACCAGCGCATCACTGTCAGG‑CAGTTcg > 6:188104/1‑149 (MQ=255) tgctCCTCGTCCGGTCACACTGTTCGCGCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCTTCACCCGCGCATCACTGTCAGG‑CAGTTcgc > 8:36496/1‑149 (MQ=39) gctCCTCGTCCGGTCACACTGTTCGCGCCGTACCGCGCGACCACCTGTTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGtt < 6:17852/112‑1 (MQ=25) ctcctcGTCCGGTCACACTGTTCGCGCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCTTCACCCGCGCATCACTGTCAGG‑CAGTTcgc < 6:9833/147‑1 (MQ=39) tcGTCCGGTCACACTGTTCGCGCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGcc < 2:158059/82‑1 (MQ=38) tcGTCCGGTCACACTGTTCGCGCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGcc > 1:158059/1‑82 (MQ=38) tcGTCCGGTCACACTGTTCGCGCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCTTCACCCGCGCATCACTGTCAGG‑CAGTTCGCGCGCtt > 6:219631/1‑149 (MQ=39) cGGTCACACTGTTCGCGCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCTTCACCCGCGCATCACTGTCAGG‑CAGTTCGCGCGCTTTGaa < 7:36496/148‑1 (MQ=39) ggTCACACTGTTCGCGCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCTTCACCCGCGCATCACTGTCAg < 1:247103/128‑1 (MQ=38) ggTCACACTGTTCGCGCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCTTCACCCGCGCATCACTGTCAg > 2:247103/1‑128 (MQ=38) cacaCTGTTCGCGCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCTTCACCCGCGCATCACTGTCAGG‑CAGTTCGCGCGCTTTGAAGGCCa > 3:66618/1‑149 (MQ=39) ggtggtCGCGCGGTACGGCGCGAACACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCTTCACCCGCGCATCACTGTCAGG‑CAGTTCGCGCGCTTTGAAGGCCAt > 8:66807/6‑147 (MQ=255) tggtCGCGCGGTACGGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCTTCACCCGCGCATCACTGTCAGG‑CAGTTCGCGCGCTTTGAAGGCCATCAg > 3:257129/4‑148 (MQ=255) tGTTCGCGCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCTTCACCCGCGCATCACTGTCAGG‑CAGTTCGCGCGCTTTGAAGGCCATCAg > 6:77197/1‑148 (MQ=39) gtCGCGCGGTACGGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCTTCACCCGCGCATCACTGTCAGG‑CAGTTCGCGCGCTTTGAAGGCCATCAGCAt > 2:160787/2‑149 (MQ=255) tCGCGCGGTACGGCGCGAACACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCTTCACCCGCGCATCACTGTCAGG‑CAGTTCGCGCGCTTTGAAGGCCATCAGCATc < 7:66807/149‑1 (MQ=255) cgcgCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCTTCACCCGCGCATCACTGTCAGG‑CAGTTCGCGCGCt > 6:88885/1‑130 (MQ=40) cgcgCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCTTCACCCGCGCATCACTGTCAGG‑CAGTTCGCGCGCt < 5:88885/130‑1 (MQ=40) cgcgCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCTTCACCCGCGCATCACTGTCAGG‑CAGTTCGCGCGCTTTGAAGGCCATCAGCATcc > 5:255030/1‑149 (MQ=39) cgcgCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCTTCACCCGCGCATCACTGTCAGG‑CAGTTCGCGCGCTTTGAAGGCCATCAGCATcc > 2:24907/1‑149 (MQ=39) gcgcGGTACGGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCTTCACCCGCGCATCACTGTCAGGCCAGTTCGCGCGCTTTGAAGGCCATCAGCATc < 1:160787/148‑1 (MQ=255) gcgcCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCTTCAccc > 7:78088/1‑100 (MQ=40) gcgcCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCTTCAccc < 8:78088/100‑1 (MQ=40) | CTTTTGTAATGCTCAATCGAGTCAGTGAAATCCCACCACGCGAGTACCAACCCCCCGGTGCCAGCTAAACTGGCGCCCCATAGTTTCAAACGCCCTAGAGCGGCCGCTGCTCCTCGTCCGGTCACACTGTTCGCGCCGTACCGCGCGACCACCTGGTCCACATAACTGGCTCCAACTTCGAAGCCCGCCGCAGCCGTAGTCATCGCGGCCGCCAGGAGTTCGGTCTTCACCCGCGCATCACTGTCAGG‑CAGTTCGCGCGCTTTGAAGGCCATCAGCATCC > NC_002947/2361583‑2361862 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |