Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,386,384 A→C 12.0% R240R (CGT→CGG)  PP_2094 ← nitrate‑binding protein NasS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,386,3840AC12.0% 104.7 / 2.5 39R240R (CGT→CGG) PP_2094nitrate‑binding protein NasS
Reads supporting (aligned to +/- strand):  ref base A (15/19);  new base C (2/3);  total (17/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTCATAATGGCCGAGCAGGCGGGGGGCTATGCTGTCCAGCGGGGTGTCCAGGTAAGCGCTTGCGCTGAGCAGTTGCGCGGTGCTGCGGCGGTTTTCCGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGGGCGCAGGCCAGGACCTTTTCCGGGTGATCCGGCCAGATCGACTGGCTGGTGGCCAGGGTGAAGCCTTGGCCTTTGGCCACCGCGTCGGCGGCCCAGGGCTCACCAA  >  NC_002947/2386236‑2386526
                                                                                                                                                    |                                                                                                                                              
ccTCATAATGGCCGAGCAGGCGGGGGGCTATGCTGTCCAGCGGGGTGTCCAGGTAAGCGCTTGCGCTGAGCAGTTGCGCGGTGCTGCGGCGGTTTTCCGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCa                                                                                                                                                >  1:154362/1‑149 (MQ=255)
     tAATGGCCGAGCAGGCGGGGGGCTATGCTGTCCAGCGGGGTGGCCAGGTAAGCGCTTGCGCTGAGCAGTTGCGCGGTGCGGCGGCGGGTGTCCGGGGTTGGGGCGGTGAAGGGGGGGGGGGGGAGGGGGGCCGTGATCGGCGCCCgggg                                                                                                                                           >  8:39556/1‑148 (MQ=255)
        tGGCCGAGCAGGCGGGGGGCTATGCTGTCCAGCGGGGTGTCCAGGTAAGCGCTTGCGCTGAGCAGTTGCGCGGTGCTGCGGCGGTTTTCCGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCt                                                                                                                                        >  5:28967/1‑149 (MQ=255)
            cGAGCAGGCGGGGGGCTATGCTGTCCAGCGGGGTGTCCAGGTAAGCGCTTGCGCTGAGCAGTTGCGCGGTGCTGCGGCGGTTTTCCGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTg                                                                                                                                    <  8:60886/149‑1 (MQ=255)
             gAGCAGGCGGGGGGCTATGCTGTCCAGCGGGGTGTCCAGGTAAGCGCTTGCGCTGAGCAGTTGCGCGGTGCTGCGGCGGTTTTCCGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTgg                                                                                                                                   >  7:123087/1‑149 (MQ=255)
              aGCAGGCGGGGGGCTATGCTGTCCAGCGGGGTGTCCAGGTAAGCGCTTGCGCTGAGCAGTTGCGCGGTGCTGCGGCGGTTTTCCGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTggg                                                                                                                                  <  2:187575/149‑1 (MQ=255)
               gCAGGCGGGGGGCTATGCTGTCCAGCGGGGTGTCCAGGTAAGCGCTTGCGCTGAGCAGTTGCGCGGTGCTGCGGCGGTTTTCCGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCCCGTGCGCTgatcaag                                                                                                                                 <  5:28578/149‑7 (MQ=255)
               gCAGGCGGGGGGCTATGCTGTCCAGCGGGGTGTCCAGGTAAGCGCTTGCGCTGAGCAGTTGCGCGGTGCTGCGGCGGTTTTCCGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTgggg                                                                                                                                 <  5:120883/149‑1 (MQ=255)
                        gggCTATGCTGTCCAGCGGGGTGTCCAGGTAAGCGCTTGCGCTGAGCAGTTGCGCGGTGCTGCGGCGGTTTTCCGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCg                                                                                                                        <  8:145144/149‑1 (MQ=255)
                         ggCTATGCTGTCCAGCGGGGTGTCCAGGTAAGCGCTTGCGCTGAGCAGTTGCGCGGTGCTGCGGCGGTTTTCCGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCCCGTGCGCTgatc                                                                                                                                    <  5:36866/136‑4 (MQ=255)
                         ggCTATGCTGTCCAGCGGGGTGTCCAGGTAAGCGCTTGCGCTGAGCAGTTGCGCGGTGCTGCGGCGGTTTTCCGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCCCGTGCGCTgatc                                                                                                                                    >  6:36866/1‑133 (MQ=255)
                              tGCTGTCCAGCGGGGTGTCCAGGTAAGCGCTTGCGCTGAGCAGTTGCGCGGTGCTGCGGCGGTTTTCCGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCa                                                                                                                     >  1:61817/1‑146 (MQ=255)
                                   tCCAGCGGGGTGTCCAGGTAAGCGCTTGCGCTGAGCAGTTGCGCGGTGCTGCGGCGGTTTTCCGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGAcgcg                                                                                                             <  2:61817/149‑1 (MQ=255)
                                   tCCAGCGGGGTGTCCAGGTAAGCGCTTGCGCTGAGCAGTTGCGCGGTGCTGCGGCGGTTTTCCGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGAcgcg                                                                                                             >  5:268468/1‑149 (MQ=255)
                                    ccAGCGGGGTGTCCAGGTAAGCGCTTGCGCTGAGCAGTTGCGCGGTGCTGCGGCGGTTTTCCGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCgg                                                                                                            <  8:226858/149‑1 (MQ=255)
                                     cAGCGGGGTGTCCAGGTAAGCGCTTGCGCTGAGCAGTTGCGCGGTGCTGCGGCGGTTTTCCGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCgg                                                                                                            <  6:268468/148‑1 (MQ=255)
                                               tCCAGGTAAGCGCTTGCGCTGAGCAGTTGCGCGGTGCTGCGGCGGTTTTCCGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGGGCGCAGGCCAg                                                                                                 <  8:123087/149‑1 (MQ=255)
                                                         cgcTTGCGCTGAGCAGTTGCGCGGTGCTGCGGCGGTTTTCCGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGGGCGCAGGCCAGGACCTTTTcc                                                                                       <  8:187050/149‑1 (MQ=255)
                                                              gcgcTGAGCAGTTGCGCGGTGCTGCGGCGGTTTTCCGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGGGCGCAGGCCAGGACCTTTTCCGGGt                                                                                   <  2:154362/148‑1 (MQ=255)
                                                                gcTGAGCAGTTGCGCGGTGCTGCGGCGGTTTTCCGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGGGCGCAGGCCAGGACCTTTTCCGGGTGAt                                                                                <  1:187728/149‑1 (MQ=255)
                                                                gcTGAGCAGTTGCGCGGTGCTGCGGCGGTTTTCCGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGGGCGCAGGCCAGGACCTTTTCCGGGTGAt                                                                                <  6:109907/149‑1 (MQ=255)
                                                                      cAGTTGCGCGGTGCTGCGGCGGTTTTCCGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGGGCGCAGGCCAGGACCTTTTCCGGGTGATCCGGc                                                                           >  8:133789/1‑148 (MQ=255)
                                                                                        gcggTTTTCCGGGCTTTGCTCGATGAATCGGCTGGCGGTTAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGGGCGCAGGCCAGGACCtt                                                                                           <  5:35959/114‑1 (MQ=255)
                                                                                        gcggTTTTCCGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGGGCGCAGGCCAGGACCtt                                                                                           >  6:35959/1‑114 (MQ=255)
                                                                                              ttCCGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGGGCGCAGGCCAGGACCTTTTCCGGGTGATCCGGCCAGATCGACTGGCTGGTGGCCatg                                                   <  7:133789/148‑3 (MQ=255)
                                                                                                 cGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGGGCGCAGGCCAGGACCTTTTCCgg                                                                                     <  6:170453/111‑1 (MQ=255)
                                                                                                 cGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGGGCGCAGGCCAGGACCTTTTCCgg                                                                                     >  5:170453/1‑111 (MQ=255)
                                                                                                         gCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGGGCGCAGGCCAGGACCTTTTCCGGGTGATCCGGCCAGATCGACTGGCTGGTGGCCAGGGTGAAGCCTTgg                                       >  1:267339/1‑149 (MQ=255)
                                                                                                            cGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGGGCGCAGGCCAGGACCTTTTCCGGGTGATCCGGCCAGATCGACTGGCTGGTGGCCAGGGTGAAGCCTTGGCCt                                    >  6:68929/1‑149 (MQ=255)
                                                                                                             gATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGGGCGCAGGCCAGGACCTTTTCCGGGTGATCCGGCCAGATCGACTGGCTGGTGGCCAGGGTGAAGCCTTg                                        >  3:218529/1‑144 (MQ=255)
                                                                                                             gATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGGGCGCAGGCCAGGACCTTTTCCGGGTGATCCGGCCAGATCGACTGGCTGGTGGCCAGGGTGAAGCCTTg                                        <  4:218529/144‑1 (MQ=255)
                                                                                                             gATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGGGCGCAGGCCAGGACCTTTTCCGGGTGATCCGGCCAGATCGACTGGCTGGTGGCCAGGGTGAAGCCTTGGCCtt                                   >  5:218966/1‑149 (MQ=255)
                                                                                                                gAATCGGCTGGCGGGGCGGAGCGCCTTGATCCCCGCCCGGGCGGTGTTGGGGTATTGCCCGGAAAAGCCGCGGGCGCAGGCCTGGACCTTTTCCGGGGGACCCGGCCAGATCGACTGGCGGGTGGCCCGGGTGAAGCCTTGGCCTTTg                                 <  4:52366/148‑1 (MQ=255)
                                                                                                                  aTCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGCGCGCAGGCCAGGACCTTTTCCGGGTGATCCGGCCAGATCGACTGGCTGGTGGCCAGGGTGAAGCCTTGGCCGTTCGc                               >  2:234718/1‑148 (MQ=255)
                                                                                                                      gCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGGGCGCAGGCCAGGACCTTTTCCGGGTGATCCGGCCAGATCGACTGGCTGGTGGCCAGGGTGAAGCCTTGGCCTTTGGCCACcg                          <  6:218966/149‑1 (MQ=255)
                                                                                                                             gTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGGGCGCAGGCCAGGACCTTTTCCGGGTGATCCGGCCAGATCGACTGGCTGGTGGCCAGGGTGAAGCCTTGGCCTTTGGCCACCGCGTcggc                   <  6:28967/149‑1 (MQ=255)
                                                                                                                                      ggcTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGGGCGCAGGCCAGGACCTTTTCCGGGTGATCCGGCCAGATCGACTGGCTGGTGGCCAGGGTGAAGCCTTGGCCTTTGGCCACCGCGTCGGCGGCCCAggg          <  5:168384/147‑1 (MQ=255)
                                                                                                                                       ccTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGGGCGCAGGCCAGGACCTTTTCCGGGTGATCCGGCCAGATCGACTGGCTGGTGGCCAGGGTGAAGCCTTGGCCTTTGGCCACCGCGTCGGCGGCCCAGGGc         >  5:64307/1‑149 (MQ=255)
                                                                                                                                        cTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGGGCGCAGGCCAGGACCTTTTCCGGGTGATCCGGCCAGATCGACTGGCTGGTGGCCAGGGTGAAGCCTTGGCCTTTGGCCACCGCGTCGGCGGCCCAGGGCt        >  3:215864/1‑149 (MQ=255)
                                                                                                                                              cAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGGGCGCAGGCCAGGACCTTTTCCGGGTGATCCGGCCAGATCGACTGGCTGGTGGCCAGGGTGAAGCCTTGGCCTTTGGCCACCGCGTCGGCGGCCCAGGGCTCACCaa  >  3:107896/1‑149 (MQ=255)
                                                                                                                                                    aCGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGGGCGCAGGCCAGGACCTTTTCCGGGTGATCCGGCCAGATCGACTGGCTGGTGGCCAGGGTGAAGCCTTGGCCTTTg                                 >  3:204953/1‑112 (MQ=255)
                                                                                                                                                    aCGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGGGCGCAGGCCAGGACCTTTTCCGGGTGATCCGGCCAGATCGACTGGCTGGTGGCCAGGGTGAAGCCTTGGCCTTTg                                 <  4:204953/112‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
CCTCATAATGGCCGAGCAGGCGGGGGGCTATGCTGTCCAGCGGGGTGTCCAGGTAAGCGCTTGCGCTGAGCAGTTGCGCGGTGCTGCGGCGGTTTTCCGGGCTTTGCTCGATGAATCGGCTGGCGGTGAGGATCGCCTTGATCAGCGCACGGGCGCTGTTGGGGTATTGCTCGGCAAAGACGCGGGCGCAGGCCAGGACCTTTTCCGGGTGATCCGGCCAGATCGACTGGCTGGTGGCCAGGGTGAAGCCTTGGCCTTTGGCCACCGCGTCGGCGGCCCAGGGCTCACCAA  >  NC_002947/2386236‑2386526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑