Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,398,675 T→A 10.3% T328S (ACC→TCC)  PP_2100 ← two‑component system sensor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,398,6750TA10.3% 99.3 / 3.8 39T328S (ACC→TCC) PP_2100two‑component system sensor protein
Reads supporting (aligned to +/- strand):  ref base T (16/19);  new base A (2/2);  total (18/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.87e-01

CCTGCTTTGCCGGCGCTGGCCAGTGCGGCGATGACATCGGCCCGGCCATCCTTGAGGCGAGCGACCATGTCGGCAATGCCACTGGCGCGCTGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGACAGTGGTGCAGCCGCGTCATCGGCTGCCACGCGCATCACGGGGTGCTTCTCCAGCCACTGCTGCTCGGCCGCGCTCAATTGCAGTTGGCGATCGCTCAGCAACAG  >  NC_002947/2398530‑2398816
                                                                                                                                                 |                                                                                                                                             
ccTGCTTTGCCGGCGCTGGCCAGTGCGGCGATGACATCGGCCCGGCCATCCTTGAGGCGAGCGACCATGTCGGCAATGCCACTGGCGCGCTGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGAt                                                                                                                                            <  6:223329/149‑1 (MQ=255)
  tGCTTTGCCGGCGCTGGCCAGTGCGGCGATGACATCGGCCCGGCCATCCTTGAGGCGAGCGACCATGTCGGCAATGCCACTGGCGCGCTGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGATGc                                                                                                                                          >  3:83103/1‑149 (MQ=255)
         ccGGCGCTGGCCAGTGCGGCGATGACATCGGCCCGGCCATCCTTGAGGCGAGCGACCATGTCGGCAATGCCACTGGCGCGCTGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGt                                                                                                                                               >  2:47695/1‑137 (MQ=255)
         ccGGCGCTGGCCAGTGCGGCGATGACATCGGCCCGGCCATCCTTGAGGCGAGCGACCATGTCGGCAATGCCACTGGCGCGCTGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGATGCCTCGGa                                                                                                                                    >  6:20532/1‑148 (MQ=255)
         ccGGCGCTGGCCAGTGCGGCGATGACATCGGCCCGGCCATCCTTGAGGCGAGCGACCATGTCGGCAATGCCACTGGCGCGCTGCACTTCGAAACGCAACACGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGt                                                                                                                                               <  1:47695/137‑1 (MQ=255)
           ggCGCTGGCCAGTGCGGCGATGACATCGGCCCGGCCATCCTTGAGGCGAGCGACCATGTCGGCAATGCCACTGGCGCGCTGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGATGCCTCGGAAAt                                                                                                                                 <  5:37097/149‑1 (MQ=255)
                  gCCAGTGCGGCGATGACATCGGCCCGGCCATCCTTGAGGCGAGCGACGATGTCGGCAATGCCACTGGCGCGCTGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTccc                                                                                                                            <  7:222458/147‑1 (MQ=255)
                  gCCAGTGCGGCGATGACATCGGCCCGGCCATCCTTGAGGCGAGCGACCATGTCGGCAATGCCACTGGCGCGCTGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTccc                                                                                                                            <  3:139572/147‑1 (MQ=255)
                           gcgATGACATCGGCCCGGCCATCCTTGAGGCGAGTGACCATGTCGGCAATGCCACTGGCGCGCTGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTCCCGAATCATCGaa                                                                                                                 <  2:184027/149‑1 (MQ=255)
                           gcgATGACATCGGCCCGGCCATCCTTGAGGCGAGCGACCATGTCGGCAATGCCACTGGCGCGCTGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTCCCGAATCATCGaa                                                                                                                 <  4:83103/149‑1 (MQ=255)
                               tGACATCGGCCCGGCCATCCTTGAGGCGAGCGACCATGTCGGCAATGCCACTGGCGCGCTGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTCCCGAATCATCGAAGTAg                                                                                                             <  5:20532/149‑1 (MQ=255)
                                   aTCGGCCCGGCCATCCTTGAGGCGAGCGACCATGTCGGCAATGCCACTGGCGCGCTGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGa                                                                                                           >  3:89662/1‑147 (MQ=255)
                                   aTCGGCCCGGCCATACTTGAGGCGAGCGACCATGTCGGCAATGCCACTGGCGCGCTGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTCGCGAATCATCGAAGTAGGACa                                                                                                         >  3:244483/1‑149 (MQ=255)
                                      ggcccggccATCCTTGAGGCGAGCGACCATGTCGGCAATGCCACTGGCGCGCTGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGATGCCt                                                                                                                                        >  1:127845/1‑115 (MQ=255)
                                      ggcccggccATCCTTGAGGCGAGCGACCATGTCGGCAATGCCACTGGCGCGCTGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGATGCCt                                                                                                                                        <  2:127845/115‑1 (MQ=255)
                                        cccggccATCCTTGAGGCGAGCGACCATGACGGCAATGCCACTGGCGCGCTGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGACAgtggt                                                                                                    <  2:42184/149‑1 (MQ=255)
                                          cggccATCCTTGAGGCGAGCGACCATGTCGGCAATGCCACTGGCGCGCTGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGACAgtggtg                                                                                                   >  8:38154/1‑148 (MQ=255)
                                                      aGGCGAGCGACCATGTCGGCAATGCCACTGGCGCGCTGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGACAGTGGTGCAGCCGCGTCATc                                                                                      >  2:245173/1‑149 (MQ=255)
                                                                 cATGTCGGCAATGCCACTGGCGCGCTGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGACAGTGGTGCAGCCGCGTCATCGGCTGCCAcgc                                                                           >  1:193923/1‑149 (MQ=255)
                                                                       ggCAATGCCACTGGCGCGCTGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGACAGTGGTGCAGCCGCGTCATCGGCTGCCACGCGCATc                                                                      <  1:245173/148‑1 (MQ=255)
                                                                           aTGCCACTGGCGCGCTGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGACAGTGGTGCAGCCGCGTCATCGGCTGCCACGCGCATCACggg                                                                 <  2:193923/149‑1 (MQ=255)
                                                                                agtGGCGCGCTGCACTTCGATTCGCAACGCGGTGCGCAGGGGAATGAGTTCCAGCAGGTCGGCGGAGATGCGTGGGAAATGTCCCGTATCATCGAAGTAGGAGTGTGGTGGAGCCGCGTCATCg                                                                                     <  8:13052/122‑1 (MQ=255)
                                                                                aCTGGCGCTCTGCACTTCGATACGCAACCAGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGAGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGAGAGTGGTGCATCCGCTTCATCg                                                                                     <  8:9101/124‑1 (MQ=255)
                                                                                aCTGGCGCGCTGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGACAGTGGTGCAGCCGCGTCATCGGCTGCCACGCGCATCACGGGGTGCt                                                            >  2:197877/1‑149 (MQ=255)
                                                                                aCTGGCGCGCTGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGAGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGACAGTGGTGCAGCCGCGTCATCg                                                                                     >  7:13052/1‑124 (MQ=255)
                                                                                aCTGGCGCGCTGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGAGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGACAGTGGTGCAGCCGCGTCATCg                                                                                     >  7:9101/1‑124 (MQ=255)
                                                                                    gcgcgcTGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGACAGTGGTGCAGCCGCGTCATCGGCTGCCACGCGCATCACGGGGTGCTtc                                                          <  7:38154/147‑1 (MQ=255)
                                                                                          tGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGACAGTGGTGCAGCCGCGTCATCGGCTGCCACGCGCa                                                                        >  8:242595/1‑127 (MQ=255)
                                                                                          tGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGACAGTGGTGCAGCCGCGTCATCGGCTGCCACGCGCa                                                                        <  7:242595/127‑1 (MQ=255)
                                                                                             aCTTCGAAACGCAACCCGCTGCGCAGGCGAATGACTTCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGACAGTGGTGCAGCCGCGTCATCGGCTGCCACGCGCATCACGGGGTGCTTCTCCAGCCActg                                               <  2:111989/149‑1 (MQ=255)
                                                                                                         aaCCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGACAGTGGTGCAGCCGCGTCATCTGCTGCCACGCGCATCACGGGGTGCTTCTCCAGCCACTGCTGCTCGGCcgc                                   <  4:89662/149‑1 (MQ=255)
                                                                                                                tGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGACAGTGGTGCAGCCGCGTCATCGGCTGCCACGCGCATCACGGGGTGCTTCTCCAGCCACTGCTGCTCGGCCGCGCTCAAt                            <  1:197877/149‑1 (MQ=255)
                                                                                                                         gAATGAGTTCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGACAGTGGTGCAGCCGCGTCATCGGCTGCCACGCGCATCACGGGGTGCTTCTCCAGCCACTGCTGCTCGGCCGCGCTCAATTGCAGTTgg                   >  8:103893/1‑149 (MQ=255)
                                                                                                                         gAATGAGTTCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGACAGTGGTGCAGCCGCGTCATCGGCTGCCACGCGCATCACGGGGTGCTTCTCCAGCCACTGCTGCTCGGCCGCGCTCAATTGCAGTTgg                   >  5:137005/1‑149 (MQ=255)
                                                                                                                              aGTTCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGACAGTGGTGCAGCCGCGTCATCGGCTGCCACGCGCATCACGGGGTGCTTCTCCAGCCACTGCTGCTCGGCCGCGCTCAATTGCAGTTGGCGATc              >  8:141578/1‑149 (MQ=255)
                                                                                                                                ttCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGACAGTGGTGCAGCCGCGTCATCGGCTGCCACGCGCATCACGGGGTGCTTCTCCAGCCACTGCTGCTCGGCCGCGCTCAATTGCAGTTGGCGa                <  4:264161/145‑1 (MQ=255)
                                                                                                                                ttCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGACAGTGGTGCAGCCGCGTCATCGGCTGCCACGCGCATCACGGGGTGCTTCTCCAGCCACTGCTGCTCGGCCGCGCTCAATTGCAGTTGGCGAtag             >  3:264161/1‑146 (MQ=255)
                                                                                                                                ttCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGACAGTGGTGCAGCCGCGTCATCGGCTGCCACGCGCATCACGGGGTGCTTCTCCAGCCACTGCTGCTCGGCCGCGCTCAATTGCAGTTGGCGATCGc            >  4:215865/1‑149 (MQ=255)
                                                                                                                                          tCGGCGGTGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGACAGTGGTGCAGCCGCGTCATCGGCTGCCACGCGCATCACGGGGTGCTTCTCCATCCACTGCTGCTCGGCCGCGCTCAATTGCAGTTGGCGATCGCTCAGCAACAg  <  4:244483/149‑1 (MQ=255)
                                                                                                                                             gcggTGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGACAGTGGTGCAGCCGCGTCATCGGCTGCCACGCGCATCACGGGGTGCTTCTCCAGCCACTGCTGCTCGGCCGCGCTCAATTGCAGTTGGCGATc              <  7:52992/134‑1 (MQ=255)
                                                                                                                                             gcggTGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGACAGTGGTGCAGCCGCGTCATCGGCTGCCACGCGCATCACGGGGTGCTTCTCCAGCCACTGCTGCTCGGCCGCGCTCAATTGCAGTTGGCGATc              >  8:52992/1‑134 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
CCTGCTTTGCCGGCGCTGGCCAGTGCGGCGATGACATCGGCCCGGCCATCCTTGAGGCGAGCGACCATGTCGGCAATGCCACTGGCGCGCTGCACTTCGAAACGCAACCCGGTGCGCAGGCGAATGAGTTCCAGCAGGTCGGCGGTGATGCCTCGGAAATGTCCCGAATCATCGAAGTAGGACAGTGGTGCAGCCGCGTCATCGGCTGCCACGCGCATCACGGGGTGCTTCTCCAGCCACTGCTGCTCGGCCGCGCTCAATTGCAGTTGGCGATCGCTCAGCAACAG  >  NC_002947/2398530‑2398816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑