Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,445,681 G→A 15.4% intergenic (‑170/‑46) lexA‑I ← / → PP_2144 transcriptional repressor/TetR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,445,6810GA15.4% 91.5 / 8.5 39intergenic (‑170/‑46)lexA‑I/PP_2144transcriptional repressor/TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (16/17);  new base A (2/4);  total (18/21)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01

TACAGTGACTGGAATTATATACAGTACTGGGTGCGCTGCAAGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGTTGAACGCATTCTCGACGCTGCCGAGCAGCTGTTCGCGGAAAGGGGGTTCGCCGAAACCTCGTTGCGGCTGAT  >  NC_002947/2445533‑2445818
                                                                                                                                                    |                                                                                                                                         
tACAGTGACTGGAATTATATACAGTACTGGGTGCGCTGCAAGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTAACCGAGCCTTGACAGAAGCAGGGCTGAAACg                                                                                                                                           >  8:80429/1‑149 (MQ=255)
  cAGTGACTGGAATTATATACAGTACTGGGTGCGCTGCAAGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGt                                                                                                                                          <  3:163139/148‑1 (MQ=255)
       aCTGGAATTATATACAGTACTGGGTGCGCTGCAAGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACATACGtt                                                                                                                                     <  8:181925/148‑1 (MQ=255)
           gAATTATATACAGTACTGGGTGCGCTGCAAGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACATACGTTTCagc                                                                                                                                <  4:88242/149‑3 (MQ=255)
                   taCAGTACTGGGTGCGCTGCAAGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGt                                                                                                                         <  7:176108/148‑1 (MQ=255)
                    aCAGTACTGGGTGCGCTGCAAGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCACACACCTGttt                                                                                                                       >  7:8260/1‑149 (MQ=255)
                    aCAGTACTGGGTGCGCTGCAAGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGttt                                                                                                                       >  5:77800/1‑149 (MQ=255)
                     cAGTACTGGGTGCGCTGCAAGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGttt                                                                                                                       <  7:80429/148‑1 (MQ=255)
                     cAGTACTGGGTGCGCTGCAAGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACATACGTTTCag                                                                                                                                 <  3:232813/138‑2 (MQ=255)
                     cAGTACTGGGTGCGCTGCAAGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACATACGTTTCag                                                                                                                                 >  4:232813/1‑137 (MQ=255)
                        tACTGGGTGCGCTGCAAGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCt                                                                                                                   >  4:130742/1‑149 (MQ=255)
                              gTGCGCTGCAAGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGAcgga                                                                                                             <  2:35698/149‑1 (MQ=255)
                                        aGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGAcggagtagtcggag                                                                                                   <  7:11850/149‑1 (MQ=255)
                                           cAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGAcggagtagtcggagtag                                                                                                <  6:77800/149‑1 (MQ=255)
                                             aaaGGCGCAATAAACAGAAGAGGTGGAGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGAcggagtagtcggagtagt                                                                                               >  1:145037/1‑148 (MQ=255)
                                                    cAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGccc                                                                                       <  1:126483/149‑1 (MQ=255)
                                                     aaTAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTAGGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGccc                                                                                       >  7:141625/1‑148 (MQ=255)
                                                      aTAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCaa                                                                                     >  7:125642/1‑149 (MQ=255)
                                                          aCAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCg                                                                                  >  3:11115/1‑148 (MQ=255)
                                                           cAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATg                                                                                                                                       >  6:105835/1‑94 (MQ=255)
                                                           cAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATg                                                                                                                                       <  5:105835/94‑1 (MQ=255)
                                                           cAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGa                                                                                <  4:11115/149‑1 (MQ=255)
                                                                  ggTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGtt                                                                         <  8:141625/149‑1 (MQ=255)
                                                                                  ggCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAAcc                                                                            <  8:81887/130‑1 (MQ=255)
                                                                                  ggCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAAcc                                                                            >  7:81887/1‑130 (MQ=255)
                                                                                  ggCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGTTGAACGCATtctc                                                             >  8:92037/1‑145 (MQ=255)
                                                                                  ggCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGTTGAACGCATtctc                                                             <  7:92037/145‑1 (MQ=255)
                                                                                         tGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGTTGAACGCATTCTCGACGCTGcc                                                    >  5:136314/1‑147 (MQ=255)
                                                                                           gcgcTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGTTGAACGCATTCTCGACGCTGCCGag                                                 >  8:119807/1‑148 (MQ=255)
                                                                                               ttttAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGAcg                                                                                                               >  4:190581/1‑82 (MQ=255)
                                                                                               ttttAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGAcg                                                                                                               <  3:190581/82‑1 (MQ=255)
                                                                                                 ttAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGAcggagtagtcggagtag                                                                                                <  7:159301/95‑1 (MQ=255)
                                                                                                 ttAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGAcggagtagtcggagtag                                                                                                >  8:159301/1‑95 (MQ=255)
                                                                                                     aTGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGTTGAACGCATTCTCGACGCTGCCGAGCAGCTGTTcgc                                      <  2:101390/149‑1 (MQ=255)
                                                                                                            ccGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGTTGAACGCATTCTCGACGCTGCCGAGCAGCTGTTCGCGGAAAgg                               <  7:119807/149‑1 (MQ=255)
                                                                                                                  aGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGTTGAACGCATTCTCGACGCTGCCGAGCAGCTGTTCGCGGAAAGGGGGTTc                         >  6:101876/1‑149 (MQ=255)
                                                                                                                         gAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGTTGAACGCATTCTCGACGCTGCCGAGCAGCTGTTCGCGGAAAGGGGGTTCGCCGaaa                  <  2:145037/149‑1 (MQ=255)
                                                                                                                                         aGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGTTGAACGCATTCTCGACGCTGCCGAGCAGCTGTTCGCGGAAAGGGGGTTCGCCGAAACCTCGTTGCGGCTGAt  >  2:10967/1‑149 (MQ=255)
                                                                                                                                            tttGAAACATACGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGTTGAACGCATTCTCGACGCTGCCGagca                                               >  5:145182/3‑101 (MQ=255)
                                                                                                                                            tttGAAACATACGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGTTGAACGCATTCTCGACGCTGCCGagca                                               <  6:145182/99‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                         
TACAGTGACTGGAATTATATACAGTACTGGGTGCGCTGCAAGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGTTGAACGCATTCTCGACGCTGCCGAGCAGCTGTTCGCGGAAAGGGGGTTCGCCGAAACCTCGTTGCGGCTGAT  >  NC_002947/2445533‑2445818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑