| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,445,684 | T→C | 16.4% | intergenic (‑173/‑43) | lexA‑I ← / → PP_2144 | transcriptional repressor/TetR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,445,684 | 0 | T | C | 16.4% | 81.7 / 9.3 | 38 | intergenic (‑173/‑43) | lexA‑I/PP_2144 | transcriptional repressor/TetR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (15/16); new base C (2/4); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01 | |||||||||||
ACTGGAATTATATACAGTACTGGGTGCGCTGCAAGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGTTGAACGCATTCTCGACGCTGCCGAGCAGCTGTTCGCGGAAAGGGGGTTCGCCGAAACCTCGTTGCGGCTGAT > NC_002947/2445540‑2445818 | aCTGGAATTATATACAGTACTGGGTGCGCTGCAAGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACATACGtt < 8:181925/148‑1 (MQ=255) gAATTATATACAGTACTGGGTGCGCTGCAAGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACATACGTTTCagc < 4:88242/149‑3 (MQ=255) taCAGTACTGGGTGCGCTGCAAGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGt < 7:176108/148‑1 (MQ=255) aCAGTACTGGGTGCGCTGCAAGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCACACACCTGttt > 7:8260/1‑149 (MQ=255) aCAGTACTGGGTGCGCTGCAAGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGttt > 5:77800/1‑149 (MQ=255) cAGTACTGGGTGCGCTGCAAGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGttt < 7:80429/148‑1 (MQ=255) cAGTACTGGGTGCGCTGCAAGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACATACGTTTCag < 3:232813/138‑2 (MQ=255) cAGTACTGGGTGCGCTGCAAGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACATACGTTTCag > 4:232813/1‑137 (MQ=255) tACTGGGTGCGCTGCAAGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCt > 4:130742/1‑149 (MQ=255) gTGCGCTGCAAGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGAcgga < 2:35698/149‑1 (MQ=255) aGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGAcggagtagtcggag < 7:11850/149‑1 (MQ=255) cAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGAcggagtagtcggagtag < 6:77800/149‑1 (MQ=255) aaaGGCGCAATAAACAGAAGAGGTGGAGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGAcggagtagtcggagtagt > 1:145037/1‑148 (MQ=255) cAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGccc < 1:126483/149‑1 (MQ=255) aaTAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTAGGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGccc > 7:141625/1‑148 (MQ=255) aTAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCaa > 7:125642/1‑149 (MQ=255) aCAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCg > 3:11115/1‑148 (MQ=255) cAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATg > 6:105835/1‑94 (MQ=255) cAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATg < 5:105835/94‑1 (MQ=255) cAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGa < 4:11115/149‑1 (MQ=255) ggTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGtt < 8:141625/149‑1 (MQ=255) ggCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAAcc < 8:81887/130‑1 (MQ=255) ggCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAAcc > 7:81887/1‑130 (MQ=255) ggCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGTTGAACGCATtctc > 8:92037/1‑145 (MQ=255) ggCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGTTGAACGCATtctc < 7:92037/145‑1 (MQ=255) tGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGTTGAACGCATTCTCGACGCTGcc > 5:136314/1‑147 (MQ=255) gcgcTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGTTGAACGCATTCTCGACGCTGCCGag > 8:119807/1‑148 (MQ=255) ttttAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGAcg > 4:190581/1‑82 (MQ=255) ttttAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGAcg < 3:190581/82‑1 (MQ=255) ttAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGAcggagtagtcggagtag > 8:159301/1‑95 (MQ=255) ttAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGAcggagtagtcggagtag < 7:159301/95‑1 (MQ=255) aTGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGTTGAACGCATTCTCGACGCTGCCGAGCAGCTGTTcgc < 2:101390/149‑1 (MQ=255) ccGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGTTGAACGCATTCTCGACGCTGCCGAGCAGCTGTTCGCGGAAAgg < 7:119807/149‑1 (MQ=255) aGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGTTGAACGCATTCTCGACGCTGCCGAGCAGCTGTTCGCGGAAAGGGGGTTc > 6:101876/1‑149 (MQ=255) gAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGTTGAACGCATTCTCGACGCTGCCGAGCAGCTGTTCGCGGAAAGGGGGTTCGCCGaaa < 2:145037/149‑1 (MQ=255) aGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGTTGAACGCATTCTCGACGCTGCCGAGCAGCTGTTCGCGGAAAGGGGGTTCGCCGAAACCTCGTTGCGGCTGAt > 2:10967/1‑149 (MQ=255) tttGAAACATACGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGTTGAACGCATTCTCGACGCTGCCGagca < 6:145182/99‑1 (MQ=255) tttGAAACATACGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGTTGAACGCATTCTCGACGCTGCCGagca > 5:145182/3‑101 (MQ=255) | ACTGGAATTATATACAGTACTGGGTGCGCTGCAAGCCAAAAGGCGCAATAAACAGAAGAGGTGGCGCTTGCAGTGGGCCGGCTGGCGCTTTTAAATGGCGACCGCCCAGTCACCGAGCCTTGACAGAAGCAGGGCTGAAACGTATGTTTCAAACACCTGTTTGTCTGACGGAGTAGTCGGAGTAGTCATGGCCCAATCGGAAACCGTTGAACGCATTCTCGACGCTGCCGAGCAGCTGTTCGCGGAAAGGGGGTTCGCCGAAACCTCGTTGCGGCTGAT > NC_002947/2445540‑2445818 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |