Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 262,944 C→G 9.5% intergenic (+179/‑306) PP_0211 → / → gabD‑I hypothetical protein/succinate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947262,9440CG 9.5% 151.3 / 7.8 53intergenic (+179/‑306)PP_0211/gabD‑Ihypothetical protein/succinate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (24/23);  new base G (2/3);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.51e-01

AAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCTCCCACAAC  >  NC_002947/262799‑263086
                                                                                                                                                 |                                                                                                                                              
aaGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAgg                                                                                                                                             <  7:144694/149‑1 (MQ=255)
aaGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAgg                                                                                                                                             <  1:233390/149‑1 (MQ=255)
  gCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGc                                                                                                                                           >  4:60580/1‑149 (MQ=255)
        cgacCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTgct                                                                                                                                      >  8:82408/1‑148 (MQ=255)
           ccGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTgctgagc                                                                                                                                  <  3:60580/149‑1 (MQ=255)
           ccGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGCCCTGATGCCGTgctgaaa                                                                                                                                  <  3:51751/149‑3 (MQ=255)
           ccGCCTAGCCAACGAACTGGCCGAGGGGAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGCCCTGATGCCGTgctgaaa                                                                                                                                  <  5:277553/149‑3 (MQ=255)
            cGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGCCCTGATGCCGTgctgaaac                                                                                                                                 <  5:76230/149‑4 (MQ=255)
              ccTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTTCTGCGCGCAACTGGGCAACGTGCCACCGATTACTACGCCGATGACTACAGCATCATCGTTTCAGAAGGGCATCAGGGCGTgctgaggtga                                                                                                                               <  2:229213/149‑1 (MQ=255)
                                    ggTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTGAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCt                                                                                                          <  2:265211/148‑1 (MQ=255)
                                         ccTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCa                                                                                                    <  7:82408/149‑1 (MQ=255)
                                          cTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGTGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCa                                                                                                    >  6:95003/1‑148 (MQ=255)
                                          cTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCAt                                                                                                   <  6:10935/149‑1 (MQ=255)
                                                tttGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTc                                                                                                           >  1:1326/1‑135 (MQ=255)
                                                tttGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTc                                                                                                           <  2:1326/135‑1 (MQ=255)
                                                     cGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGa                                                                                        <  5:110345/149‑1 (MQ=255)
                                                       acacCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAgg                                                                                      >  8:208553/1‑149 (MQ=255)
                                                         cccccGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAggg                                                                                     <  4:261241/147‑1 (MQ=255)
                                                          ccccGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGAAGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGcc                                                                                   >  3:199653/1‑149 (MQ=255)
                                                            ccGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGt                                                                                 >  5:9511/1‑149 (MQ=255)
                                                            ccGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGt                                                                                 >  6:179049/1‑149 (MQ=255)
                                                            ccGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGt                                                                                 <  2:62019/149‑1 (MQ=255)
                                                                     ggTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAgg                                                                                      <  2:60181/135‑1 (MQ=255)
                                                                     ggTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAgg                                                                                      >  1:60181/1‑135 (MQ=255)
                                                                     ggTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTcccc                                                                         <  4:227211/148‑1 (MQ=255)
                                                                             gcgcAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACAt                                                                >  4:138212/1‑149 (MQ=255)
                                                                                 aaCTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACAtttct                                                            >  4:124742/1‑149 (MQ=255)
                                                                                 aaCTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACAtttct                                                            >  7:205315/1‑149 (MQ=255)
                                                                                  aCTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACAtttctt                                                           >  2:234792/1‑149 (MQ=255)
                                                                                   cTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACAtttcttt                                                          <  3:124742/149‑1 (MQ=255)
                                                                                         aaagagCCACATATTACGATCCCGATGAAAACAGCATCATCAATTCAGCAAGGCAAAAGGCCGTCCTGACCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCATCCTGGAGGGCCGTCGGTCCACCCTTCACATTTCTATCAGtt                                                     >  8:180914/6‑148 (MQ=255)
                                                                                             tGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTc                                                <  8:205315/149‑1 (MQ=255)
                                                                                               ccACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGt                                              >  3:252057/1‑149 (MQ=255)
                                                                                               ccACCGATTACGATGCCGATGACAACACCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTc                                                                                             >  8:211903/1‑102 (MQ=255)
                                                                                               ccACCGATTACGATGCCGATGACAACACCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTc                                                                                             <  7:211903/102‑1 (MQ=255)
                                                                                                   cGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTaa                                          <  5:179049/149‑1 (MQ=255)
                                                                                                    gATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAAc                                         <  3:138212/149‑1 (MQ=255)
                                                                                                     aTTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTTCCCCATTCACATTTCTTTCAGTTCATTCGTCTAAcc                                        >  7:93520/1‑149 (MQ=255)
                                                                                                       gatgATGCTGTTGTCATCGGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTg                                      >  5:8043/4‑149 (MQ=255)
                                                                                                         cGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTa                                    >  5:70997/1‑149 (MQ=255)
                                                                                                             gCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTgg                                >  4:129115/1‑149 (MQ=255)
                                                                                                              ccGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGc                               <  6:70997/149‑1 (MQ=255)
                                                                                                                gATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGccc                             >  6:215281/1‑149 (MQ=255)
                                                                                                                gATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGccc                             <  5:95003/149‑1 (MQ=255)
                                                                                                                        caGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACATCTCCAAGGCGTTCTGATTAATCCCCACCGGGAGGGCCGTCGGTCCCCCCTACACATTAATTTAAGTTCATTCGTGTAACATGTAGTGGCAATATCGCTg                     >  1:149859/1‑149 (MQ=255)
                                                                                                                           catcatCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCa                  <  1:234792/149‑1 (MQ=255)
                                                                                                                                tcagctCAGCACGCCCTGATGCCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCa             >  8:47486/6‑149 (MQ=255)
                                                                                                                                  gTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGc           >  6:162890/1‑149 (MQ=255)
                                                                                                                                    ctCAGCACGCCCTGATGCCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCACGCCAGCTc         >  8:91351/2‑149 (MQ=255)
                                                                                                                                     tCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGATATGATTAATCACCTCCGGGAGGGCCGTCGGTCACCCCTTAACATTTCTTTCAGTTAATTCGTCTAACCTGTACTGGCCCTATAGCTGGCAAGCCAGCTcc        >  7:168767/1‑149 (MQ=255)
                                                                                                                                       aGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCTCCca      <  5:215281/149‑1 (MQ=255)
                                                                                                                                         cACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCTCCcac     >  6:76517/1‑148 (MQ=255)
                                                                                                                                           cGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCTCCcacaac  <  7:208553/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
AAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCTCCCACAAC  >  NC_002947/262799‑263086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑