Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,488,203 G→A 8.9% intergenic (+59/‑343) PP_2182 → / → PP_2183 hypothetical protein/formate dehydrogenase subunit gamma

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,488,2030GA 8.9% 152.9 / 5.2 56intergenic (+59/‑343)PP_2182/PP_2183hypothetical protein/formate dehydrogenase subunit gamma
Reads supporting (aligned to +/- strand):  ref base G (22/29);  new base A (2/3);  total (24/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

AGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGA‑CCCACCTGATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGTCGGTTTCAGAATGCGCCTG  >  NC_002947/2488056‑2488343
                                                                                                                                                   |                                                                                                                                             
aGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGt                                                                                                                                              <  3:262146/149‑1 (MQ=255)
    ccGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTgg                                                                                                                                          <  6:136105/149‑1 (MQ=255)
      gACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTgg                                                                                                                                          >  1:85643/1‑147 (MQ=255)
       aCGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAg                                                                                                                                       >  3:244665/1‑149 (MQ=255)
       aCGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTAAGTCGCGTTCTGCCCCGTAGCCATGCTGTATGGCAGACCATTTCCGGGGCTGCCGACTGGCAg                                                                                                                                       >  8:240597/1‑149 (MQ=255)
            aTCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGGTCAGTCGCTTTCTTCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCg                                                                                                                                  <  5:124794/149‑1 (MQ=255)
             tCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGCCTGCCAGACGGCAGCCCCgg                                                                                                                                 <  6:245368/149‑1 (MQ=255)
               gTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGCCTGCCAGACGGCAGCCCCGGc                                                                                                                                <  6:93570/148‑1 (MQ=255)
                   gAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATgg                                                                                                                           <  5:8297/149‑1 (MQ=255)
                       tCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGAc                                                                                                                         >  7:64188/1‑147 (MQ=255)
                             gcccaggcccagGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCg                                                                                                                 >  8:198039/1‑149 (MQ=255)
                              cccaggcccaggCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCgg                                                                                                                >  7:179653/1‑149 (MQ=255)
                              cccaggcccaggCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCgg                                                                                                                <  3:93702/149‑1 (MQ=255)
                                caggcccaggcTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGtt                                                                                                              <  2:85643/149‑1 (MQ=255)
                                  ggcccaggcTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTc                                                                                                            <  5:12920/149‑1 (MQ=255)
                                   gcccaggcTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCg                                                                                                           <  5:228524/149‑1 (MQ=255)
                                     ccaggcTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTc                                                                                                            >  4:18803/1‑146 (MQ=255)
                                        ggcTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGc                                                                                                                                         >  4:34227/1‑114 (MQ=255)
                                          cTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCg                                                                                                           >  3:97804/1‑142 (MQ=255)
                                          cTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCg                                                                                                           <  4:97804/142‑1 (MQ=255)
                                              ggCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCGGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGtg                                                                                                <  8:188097/149‑1 (MQ=255)
                                                cccccGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATTGCAGCCCATTGCCGGGGCTGCCATcc                                                                                                                                            <  4:116211/103‑2 (MQ=255)
                                                cccccGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCATcc                                                                                                                                            >  3:116211/1‑102 (MQ=255)
                                                   ccgccgccgACACCGTAACGTTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGTATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGtgcatgc                                                                                           <  7:170307/149‑1 (MQ=255)
                                                            acacCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGt                                                                                  <  7:198039/149‑1 (MQ=255)
                                                                cGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGtgcatg                                                                                            <  2:32917/135‑1 (MQ=255)
                                                                cGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGtgcatg                                                                                            >  1:32917/1‑135 (MQ=255)
                                                                          cAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGA‑CCCAcc                                                                    <  8:64188/148‑1 (MQ=255)
                                                                           aGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGA‑CCCACCTg                                                                  <  3:18803/149‑1 (MQ=255)
                                                                              cTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGA‑CCCACCTGATc                                                               >  5:8814/1‑149 (MQ=255)
                                                                                 ccGACTGAGCTCATTCGCGTTCTGCCCCGTAGCCATTCTGTATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGA‑CCCACCTGATCg                                                              <  7:240597/147‑1 (MQ=255)
                                                                                  cGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGA‑CCCACCTGATCGGc                                                            >  8:145885/1‑148 (MQ=255)
                                                                                   gACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGA‑CCCACCTGATCGGc                                                            >  7:20601/1‑147 (MQ=255)
                                                                                    aCTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCgg                                                                                                                <  1:214415/95‑1 (MQ=255)
                                                                                    aCTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCgg                                                                                                                >  2:214415/1‑95 (MQ=255)
                                                                                          cTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGA‑CCCACCTGATCGGCAAGCCGTgg                                                   <  4:244665/149‑1 (MQ=255)
                                                                                           tCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGA‑CCCACCTGATCGGCAAGCCGTgg                                                   >  2:164359/1‑148 (MQ=255)
                                                                                             aGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGA‑CCCACCTGATCGGCAAGCCGTGGc                                                  <  5:28829/147‑1 (MQ=255)
                                                                                                  cgTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGA‑CCCACCTGATCGGCAAGCCGTGGCAgatgat                                           >  5:257809/1‑149 (MQ=255)
                                                                                                   gTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGATCCCACCTGATCGGCAAGCCGTGGCAgatgat                                           <  1:164359/149‑1 (MQ=255)
                                                                                                       tGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGA‑CCCACCTGATCGGCAAGCCGTGGCAGATGATGCCgg                                      >  4:30196/1‑149 (MQ=255)
                                                                                                              tAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGA‑CCCACCTGATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTaa                               <  6:8814/149‑1 (MQ=255)
                                                                                                                gCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGA‑CCCACCTGATCGGCAAGCCGTGGCAGATGATGCCggg                                     <  2:90665/141‑1 (MQ=255)
                                                                                                                gCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGA‑CCCACCTGATCGGCAAGCCGTGGCAGATGATGCCggg                                     >  1:90665/1‑141 (MQ=255)
                                                                                                                   aTGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGA‑CCCACCTGATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACg                          >  2:44698/1‑149 (MQ=255)
                                                                                                                    tGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGA‑CCCACCTGATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGt                         >  3:89548/1‑149 (MQ=255)
                                                                                                                    tGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGA‑CCCACCTGATCGGCAAGCCGTGCCAGATGATGCCGGGGCGTAATCACGt                         <  1:7107/149‑1 (MQ=255)
                                                                                                                         gATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGA‑CCCACCTGATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGTc                    <  1:44698/149‑1 (MQ=255)
                                                                                                                          aTGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTACGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGA‑CCCACCTGATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGTCg                   <  7:145885/149‑1 (MQ=255)
                                                                                                                             tcatcccATTGCCGGTGCTGCCGTCTGTCAGGCCCGGAAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGA‑CGCACCTTATCGGCAAGCCGTTGCAGATGATGCCGGTGCGTAATCACGTGCGTCGGt                 <  8:179653/144‑1 (MQ=255)
                                                                                                                               aGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGa                                                                           <  7:39845/89‑1 (MQ=255)
                                                                                                                               aGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGa                                                                           >  8:41085/1‑89 (MQ=255)
                                                                                                                               aGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGAGCATGCAACGATGGTTTCAAGa                                                                           <  7:41085/89‑1 (MQ=255)
                                                                                                                               aGCCCATTGCCGAGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGa                                                                           >  8:39845/1‑89 (MQ=255)
                                                                                                                                 tccATTGCCGGGCCTGCCAGACGGCAGCCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGA‑CCCACCTGATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGTCGGttt               >  8:11229/2‑146 (MQ=38)
                                                                                                                                           gggCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTTGTGCATGCAACGATGGTTTCAAGA‑CCCACCTGATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGTCGGTTTCAGAATGCGCCTg  <  6:257809/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
AGGACCGACGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCGTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGACTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGA‑CCCACCTGATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGTCGGTTTCAGAATGCGCCTG  >  NC_002947/2488056‑2488343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑