Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,498,216 C→T 11.3% H39H (CAC→CAT) ‡ PP_2193 → ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,498,2160CT11.3% 113.9 / 5.9 44H39H (CAC→CAT) ‡PP_2193ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base C (25/14);  new base T (3/2);  total (28/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

CAACCCATTCTCATTTACGACTTGTCAGGAAGCTCATTCGTGCCTCTGATGCTCAAGCCCTTGCACCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACCTGCTGTACACCACAGGCGAT  >  NC_002947/2498079‑2498357
                                                                                                                                         |                                                                                                                                              
cAACCCATTCTCATTTACGACATGTCAGGAAGCTCAGTCGTGCCGCTGATGATCAAGCCCTTGCACCGCCGTCGCGGTTATTTTCCCCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCGCGGACCCCTGCACGCA‑GGGGCcgc                                                                                                                                    >  3:38992/1‑149 (MQ=255)
              ttACGACTTGTCAGGAAGCTCATTCGTGCCTCTGATGCTCAAGCCCTTGCACCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACGCA‑GGGGCcgcg                                                                                                                                   >  1:182237/1‑136 (MQ=255)
              ttACGACTTGTCAGGAAGCTCATTCGTGCCTCTGATGCTCAAGCCCTTGCACCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACGCA‑GGGGCcgcg                                                                                                                                   <  2:182237/136‑1 (MQ=255)
                  gACTTGTCAGGAAGCTCATTCGTGCCTCTGATGCTCAAGCCCTTGCACCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGG‑CCCCTGCGTGCA‑gggggccga                                                                                                                                   >  5:186336/1‑126 (MQ=255)
                  gACTTGTCAGGAAGCTCATTCGTGCCTCTGATGCTCAAGCCCTTGCACCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGG‑CCCCTGCGTGCA‑gggggccga                                                                                                                                   <  6:186336/131‑6 (MQ=255)
                                 tCATTCGTGCCTCTGATGCTCAAGCCCTTGCACCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCg                                                                                                   >  2:38386/1‑149 (MQ=255)
                                     tCGTGCCTCTGATGCTCAAGCCCTTGCACCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAg                                                                                               >  5:28466/1‑149 (MQ=255)
                                      cGTGCCTCTGATGCTCAAGCCCTTGCACCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAg                                                                                               <  1:38386/148‑1 (MQ=255)
                                              tGATGCTCAAGCCCTTGCACCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGccc                                                                                                    <  2:171904/135‑1 (MQ=255)
                                              tGATGCTCAAGCCCTTGCACCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGccc                                                                                                    >  1:171904/1‑135 (MQ=255)
                                                aTGCTCAAGCCCTTGCACCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGa                                                                                    >  2:245496/1‑149 (MQ=255)
                                                   cTCAAGCCCTTGCACCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCt                                                                                 >  5:34693/1‑149 (MQ=255)
                                                    tCAAGCCCCTGCACCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAg                                                                                         <  6:59425/140‑1 (MQ=255)
                                                    tCAAGCCCCTGCACCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAg                                                                                         >  5:59425/1‑140 (MQ=255)
                                                            ttGCACCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCCCGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGccc                                                                         >  6:34332/1‑148 (MQ=255)
                                                                aCCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCg                                                                    >  4:130478/1‑149 (MQ=255)
                                                                aCCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCACGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCg                                                                    >  5:43574/1‑149 (MQ=255)
                                                                aCCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCACGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCg                                                                    >  5:39480/1‑149 (MQ=255)
                                                                  cgccgTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCt                                                                  <  1:239244/149‑1 (MQ=255)
                                                                       tCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTg                                                                 <  8:105994/145‑1 (MQ=255)
                                                                       tCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTg                                                                 >  7:105994/1‑145 (MQ=255)
                                                                                ttttCACCATGCCTTGATGTCTTGCAGTGACGTGGCCATGTTGCTCGGACCCCTGCACGCA‑GGGGCCGCGACGGCTGCAGAACAGACCCAGATGCACGCCCGCCAGGTCCAGCTCGACCTTCCAGCCCAACCGCTGGCCCACGCGCTGa                                                    >  7:111777/1‑149 (MQ=255)
                                                                                 tttCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGAc                                                   >  5:159473/1‑149 (MQ=255)
                                                                                        aTGCCTTGTTGTCTGGCAGTGCCGTGTCCCTGTTGCGCGGCCCCCTGCCCGCT‑GGGGCCGCGACGGCTGCCGAACAGTCCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTc                                            <  4:38992/149‑1 (MQ=255)
                                                                                        aTGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTc                                                                                            >  4:136828/1‑101 (MQ=255)
                                                                                             ttGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCTGa                                       >  8:235458/1‑149 (MQ=255)
                                                                                                        cAGTGCCGTGGCCCTGTTGCACGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCTAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAggccggc                             <  6:39480/148‑1 (MQ=255)
                                                                                                        cAGTGCCGTGGCCCTGTTGCACGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAggccggc                             <  6:43574/148‑1 (MQ=255)
                                                                                                             ccGTGGCCCTGTTGCTCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGcc                                         <  5:122661/131‑1 (MQ=255)
                                                                                                             ccGTGGCCCTGTTGCTCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGcc                                         >  6:122661/1‑131 (MQ=255)
                                                                                                                    ccTGTTGCTCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACCTGCTGt                <  3:130478/149‑1 (MQ=255)
                                                                                                                       gTTGCTCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACCTGCTGTaca             >  8:126496/1‑149 (MQ=255)
                                                                                                                        ttGCTCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACCTGCTGTac              <  6:28466/147‑1 (MQ=255)
                                                                                                                           cTCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAggccggc                             >  2:222735/1‑129 (MQ=255)
                                                                                                                           cTCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAggccggc                             <  1:222735/129‑1 (MQ=255)
                                                                                                                            tCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACCTGCTGTAcacca          >  1:146844/1‑147 (MQ=255)
                                                                                                                            tCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACCTGCTGTAcacca          >  1:30202/1‑147 (MQ=255)
                                                                                                                             cGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACCTGCTGTACACCACAg       >  7:153325/1‑149 (MQ=255)
                                                                                                                              cggccccTGCGTGCAGGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGCCCCCTTTCGCCGACCAGGCCGGCCTGCACCTGCTGTACCCCACAg       >  7:160337/4‑149 (MQ=255)
                                                                                                                              cggccccTGCGTGCAGGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACCTGCTGTa               >  3:185344/4‑141 (MQ=255)
                                                                                                                              cggccccTGCGTGCAGGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACCTGCTGTa               <  4:185344/138‑1 (MQ=255)
                                                                                                                                  cccTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGAc                                                   <  3:232748/100‑1 (MQ=255)
                                                                                                                                  cccTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGAc                                                   >  4:232748/1‑100 (MQ=255)
                                                                                                                                  cccTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACCTGCTGTACACCACAGGCGAt  >  7:173785/1‑149 (MQ=255)
                                                                                                                                         |                                                                                                                                              
CAACCCATTCTCATTTACGACTTGTCAGGAAGCTCATTCGTGCCTCTGATGCTCAAGCCCTTGCACCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACGCA‑GGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACCTGCTGTACACCACAGGCGAT  >  NC_002947/2498079‑2498357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑