Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,498,778 T→C 10.2% F227L (TTC→CTC)  PP_2193 → ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,498,7780TC10.2% 101.7 / 3.7 39F227L (TTC→CTC) PP_2193ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base T (21/14);  new base C (2/2);  total (23/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.57e-01

CTGAAGGAAGCCATCGGCAAGCTGGTGCCGGGCCTTGACCTGGCGCCGGAAGGGCGCACCAATTACGGCCAGAACATGCGTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGCCATCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGG  >  NC_002947/2498631‑2498926
                                                                                                                                                   |                                                                                                                                                    
cTGAAGGAATCCATCGGCAAGCTGGTGCCGGGCCTTGACCTGGCGCCGGAATGGCTCACCAATTACGGCCACAACATGCGTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGtt                                                                                                                                                     <  1:84741/149‑1 (MQ=255)
     ggaagCCATCGGCAAGCTGGTGCCGGGCCTTGACCTGGCGCCGGAAGGGCGCACCAATTACGGCCAGAACATGCGTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACa                                                                                                                                                <  7:69988/149‑1 (MQ=255)
                             gggCCTTGACCTGGCGCCGGAAGGGCGCACCAATTACGGCCAGAACATGCGTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAgc                                                                                                                        >  2:248335/1‑149 (MQ=255)
                                 cTTGACCTGGCGCCGGAAGGGCGCACCAATTACGGCCAGAACATGCGTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGt                                                                                                                    >  4:69582/1‑149 (MQ=255)
                                 cTTGACCTGGCGCCGGAAGGGCGCACCAATTACGGCCAGAACATGCGTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGt                                                                                                                    >  8:63306/1‑149 (MQ=255)
                                  ttGACCTGGCGCCGGAAGGGCGCACCAATTACGGCCAGAACATGCGTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTc                                                                                                                   >  4:172177/1‑149 (MQ=255)
                                     aCCTGGCGCCGGAAGGGCGCACCAATTACGGCCAGAACATGCGTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGaa                                                                                                                <  3:69582/149‑1 (MQ=255)
                                       cTGGCGCCGGAAGGGCGCACCAATTACGGCCAGAACATGCGTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCg                                                                                                                                        >  7:131360/1‑123 (MQ=255)
                                       cTGGCGCCGGAAGGGCGCACCAATTACGGCCAGAACATGCGTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCg                                                                                                                                        <  8:131360/123‑1 (MQ=255)
                                            gcCGGAAGGGCGCACCAATTACGGCCAGAACATGCGTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGt                                                                                                         >  7:124439/1‑149 (MQ=255)
                                             ccGGAAGGGCGCACCAATTACGGCCAGAACATGCGTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTc                                                                                                        <  7:63306/149‑1 (MQ=255)
                                               ggAAGGGCGCACCAATTACGGCCAGAACATGCGTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTggggtgtt                                                                                                      >  1:254195/1‑142 (MQ=255)
                                                    ggCGCACCAATTACGGCCAGAACATGCGTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGgcgc                                                                                                  >  1:247267/1‑148 (MQ=255)
                                                      cgcACCAATTACGGCCAGAACATGCGTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGa                                                                                                                          <  6:97378/122‑1 (MQ=255)
                                                      cgcACCAATTACGGCCAGAACATGCGTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGa                                                                                                                          >  5:97378/1‑122 (MQ=255)
                                                         aCCAATTACGGCCAGAACATGCGTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAg                                                                                                                                               <  6:152995/98‑1 (MQ=255)
                                                         aCCAATTACGGCCAGAACATGCGTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAg                                                                                                                                               >  5:152995/1‑98 (MQ=255)
                                                          ccAATTACGGCCAGAACATGCGTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGgcgccagcgcc                                                                                           <  2:247267/149‑1 (MQ=255)
                                                              ttACGGCCAGAACATGCGTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGCCATCt                                                                                       <  3:172177/149‑1 (MQ=255)
                                                                aCGGCCAGAACATGCGTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCGAGCGCCATCTa                                                                                      <  4:131541/148‑1 (MQ=255)
                                                                  ggCCAGAACATGCGTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGCACAGCTCCCGCGGACTTTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGCCATCTAcgg                                                                                   >  5:189107/1‑149 (MQ=255)
                                                                    ccAGAACATGCGTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTc                                                                                                                                                    <  5:137642/82‑1 (MQ=255)
                                                                    ccAGAACATGCGTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTc                                                                                                                                                    >  6:137642/1‑82 (MQ=255)
                                                                     cAGAACATGCGTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGAACATCTCACGCGGCCTGTCGCGCCAGTTcca                                                                                                                                                  >  2:111172/1‑81 (MQ=255)
                                                                               gTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGCCATCTACGGCGGTGGCGCCAcc                                                                      >  1:82939/1‑149 (MQ=255)
                                                                                                     gatCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGCCATCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGa                                                >  6:236478/1‑149 (MQ=255)
                                                                                                      ttCGACGGGGGAAGCCATAACTGCTCCCGCGGACTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGCCATCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGAc                                               <  6:189107/148‑1 (MQ=255)
                                                                                                       tCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGCCATCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGAc                                               >  5:30656/1‑148 (MQ=255)
                                                                                                                   gCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGCCATCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAg                                  >  1:228757/1‑149 (MQ=255)
                                                                                                                       gAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCACCGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGCCATCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTg                              <  7:145216/149‑1 (MQ=255)
                                                                                                                                ccGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGGGTCTAAGTGCTGTCTGGCGCCAGCGCCATCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGgc                       <  1:248335/147‑1 (MQ=255)
                                                                                                                                ccGCGGCCTGTCGCGCCAGCTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGCCATCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGt                                                  >  5:258890/1‑120 (MQ=255)
                                                                                                                                ccGCGGCCTGTCGCGCCAGCTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGCCATCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGt                                                  <  6:258890/120‑1 (MQ=255)
                                                                                                                                ccGCGGCCTGTCGCGCCAGCTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGCCATCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGt                                                  <  6:252278/120‑1 (MQ=255)
                                                                                                                                ccGCGGCCTGTCGCGCCAGCTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGCCATCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGt                                                  >  5:252278/1‑120 (MQ=255)
                                                                                                                                       cTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACATCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGCCATCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCg               >  4:128146/1‑148 (MQ=255)
                                                                                                                                             cgcCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGCCATCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAAcc          >  7:8057/1‑147 (MQ=255)
                                                                                                                                               ccAGTTAGACAGCATTTAGCCGTTCAACGTCGAGCGCGTCGAAGTGCTTTCTGGCGCCAGCGCCATCTACGGCGGTGGCGCCACCGGCGGCATCCTGAACATCCTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCGTAGCAACCCAGt      >  4:94533/1‑149 (MQ=255)
                                                                                                                                                cAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGCCATCTACGGCGGTGgcgc                                                                          <  7:25541/80‑1 (MQ=255)
                                                                                                                                                cAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGCCATCTACGGCGGTGgcgc                                                                          >  8:25541/1‑80 (MQ=255)
                                                                                                                                                   ttCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGCCATCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTggg  >  6:234517/1‑149 (MQ=255)
                                                                                                                                                   ttCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGCCATCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTggg  <  8:8057/149‑1 (MQ=255)
                                                                                                                                                   ttCGACAGCATTTCGCCGTTCAACGTCGAGCGCGGCGAAGTGCTGTCTGGCGCCAGCGCCATCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTggg  <  4:46811/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CTGAAGGAAGCCATCGGCAAGCTGGTGCCGGGCCTTGACCTGGCGCCGGAAGGGCGCACCAATTACGGCCAGAACATGCGTGGGCGCAACGTGCTGGTGATGATCGACGGGGTCAGCCAGAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGCCATCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGG  >  NC_002947/2498631‑2498926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑