Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,523,475 G→T 10.3% G66V (GGT→GTT)  PP_2213 → acyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,523,4750GT10.3% 109.1 / 4.2 39G66V (GGT→GTT) PP_2213acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base G (20/15);  new base T (2/2);  total (22/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.87e-01

GCCGAGGCCGCTCGTACGTTCGACCATGACCAGGCCGTTGTGACGGCATTGCACGGCAACCTTGAAGCCCTTAATGCCTGTGTCGAATGCTGTGACCGGCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGGCTGCTCGGTTCGCCAATGTGTTAAAGGCGCAGGGGGTAGGCGCCGGAGACCGGGTTGCCGGCTTGATGCCGCGTACCCCTGAACTGCTGGTGACCATCCTCG  >  NC_002947/2523330‑2523618
                                                                                                                                                 |                                                                                                                                               
gCCGAGGCCGCTCGTACGTTCGACCATGACCAGGCCGTTGTGACGGCATTGCACGGCAACCTTGAAGCCCTTAATGCCTGTGTCGAATGCTGTGACCGGCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGCTGTTac                                                                                                                                              >  8:17724/1‑148 (MQ=255)
 ccGAGGCCGCTCGTACGTTCGACCATGACCAGGCCGTTGTGACGGCATTGCACGGCAACCTTGAAGCCCTTAATGCCTGTGTCGAATGCTGTGACCGGCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTaa                                                                                                                                              >  6:12924/1‑148 (MQ=255)
     ggCCGCTCGTACGTTCGACCATGACCAGGCCGTTGTGACGGCATTGCACGGCAACCTTGAAGCCCTTAATGCCTGTGTCGAATGCTGTGACCGGCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTa                                                                                                                                               >  5:23985/1‑143 (MQ=255)
     ggCCGCTCGTACGTTCGACCATGACCAGGCCGTTGTGACGGCATTGCACGGCAACCTTGAAGCCCTTAATGCCTGTGTCGAATGCTGTGACCGGCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTa                                                                                                                                               <  6:23985/143‑1 (MQ=255)
       ccGCTCGTACGTTCGACCATGACCAGGCCGTTGTGACGGCATTGCACGGCAACCTTGAAGCCCTTAATGCCTGTGTCGAATGCTGTGACCGGCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGCTGTTACCATcgcg                                                                                                                                       <  7:17724/149‑1 (MQ=255)
        cGCTCTTACGTTCGACCATTAACAGGCCGTTGTTACGGCATTGCACTGCAACCTTGAAGCCCTTAATGCCTGTGTCGAATGCTGTGACCGGCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTAACAGCGCGt                                                                                                                                      <  7:104685/149‑1 (MQ=255)
             gTACGTTCGACCATGACCAGGCCGTTGTGACGGCATTGCACGGCAACCTTGAAGCCCTTAATGCCTGTGTCGAATGCTGTGACCGGCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTAACAGCGCGTGCta                                                                                                                                  >  8:192518/1‑148 (MQ=255)
              tACGTTCGACCATGACCAGGCCGTTGTGACGGCATTGCACGGCAACCTTGAAGCCCTTAATGCCTGTGTCGAATGCTGTGACCGGCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGCTGTTACCAtcgc                                                                                                                                        <  7:87972/141‑1 (MQ=255)
              tACGTTCGACCATGACCAGGCCGTTGTGACGGCATTGCACGGCAACCTTGAAGCCCTTAATGCCTGTGTCGAATGCTGTGACCGGCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGCTGTTACCAtcgc                                                                                                                                        >  8:87972/1‑141 (MQ=255)
                    cGACCATGACCAGGCCGTTGTGACGGCATTGCACGGCAACCTTGAAGCCCTTAATGCCTGTGTCGAATGCTGTGACCGGCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTc                                                                                                                           >  6:160783/1‑148 (MQ=255)
                               aGGCCGTTGTGACGGCATTGCACGGCAACCTTGAAGCCCTTAATGCCTGTGTCGAATGCTGTGACCGGCATGCAGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAg                                                                                                               >  3:157677/1‑149 (MQ=255)
                                       gtgACGGCATTGCACGGCAACCTTGAAGCCCTTAATGCCTGTGTCGAATGCTGTGACCGGCATGCCGGTGATGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAgg                                                                                                        <  4:248304/148‑1 (MQ=255)
                                          aCGGCATTGCACGGCAACCTTGAAGCCCTTAATGCCTGTGTCGAATGCTGTGACCGGCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGgctg                                                                                                     <  2:132577/148‑1 (MQ=255)
                                                    aCGGCAACCTTGAAGCCCTTAATGCCTGTGTCGAATGCTGTGACCGGCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGGCTGCTCGGTTCGcc                                                                                          <  4:191943/149‑1 (MQ=255)
                                                       gCAACCTTGAAGCCCTTAATGCCTGTGTCGAATGCTGTGACCGGCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGGCTGCTCGGTTCGCCAAt                                                                                       <  5:12924/149‑1 (MQ=255)
                                                                              tgtgACGAATGCTGTGACCGGCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGAAGGCTGCTCGGTTCGCCAATGTGTTAAAGGCGAAGGGGGTAgg                                                                >  8:222292/1‑149 (MQ=255)
                                                                                  tCGAATGCTGTGACCGGCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTAACAGAGCGTGCTATAGCTTCGATCAGATTCAGGTGCAGGCTGCTCTGTTAGCCAATGTGTTAAAGTCGCAGGGGGTAGGCGcc                                                            >  8:93142/1‑149 (MQ=255)
                                                                                    gAATGCTGTGACCGGCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGGCTGCTCGGTTCGCCAATGTGTTAAAGgcg                                                                           <  8:225619/132‑1 (MQ=255)
                                                                                    gAATGCTGTGACCGGCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGGCTGCTCGGTTCGCCAATGTGTTAAAGgcg                                                                           >  7:225619/1‑132 (MQ=255)
                                                                                    gAATGCTGTGACCGGCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGGCTGCTCGGTTCGCCAATGTGTTAAAGGCGCAGGGGGTAGGCGCCgg                                                          <  7:192518/149‑1 (MQ=255)
                                                                                     aaTGCTGTGACCGGCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGGCTGCTGGGTTCGCCAATGTGTTAAAGGCGCAGGGGGTAGGCGCCg                                                           >  7:101813/1‑147 (MQ=255)
                                                                                            tgACCGGCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGGCTGCTCGGTTCGCCAATGTGTTAAAGGCGCAGGGGGTAGGCGCCGGAGACCggg                                                  >  7:83321/1‑149 (MQ=255)
                                                                                            tgACCGGCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGGCTGCTCGGTTCGCCAATGTGTTAAAGGCGCAGGGGGTAGGCGCCGGAGACCg                                                    <  7:36615/147‑1 (MQ=255)
                                                                                                cGGCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGGCTGCTCGGTTCGCCAAt                                                                                       <  4:222166/108‑1 (MQ=255)
                                                                                                cGGCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGGCTGCTCGGTTCGCCAAt                                                                                       >  3:222166/1‑108 (MQ=255)
                                                                                                cGGCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGGCTGCTCGGTTCGCCAATGTGTTAAAGGCGCAGGGGGTAGGCGCCGGAGACCggggtac                                              >  8:198883/1‑145 (MQ=255)
                                                                                                 ggCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGGCTGCTCGGTTCGCCAATGTGTTAAAGGCGCAGGGGGTAGGCGCCGGAGACCGGGTTGcc                                             <  5:160783/149‑1 (MQ=255)
                                                                                                     tGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGGCTGCTCGGTTCGCCAATGTGTTAAAGGCGCAGGGGGTAGGCGCCg                                                           <  1:79998/131‑1 (MQ=255)
                                                                                                     tGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGGCTGCTCGGTTCGCCAATGTGTTAAAGGCGCAGGGGGTAGGCGCCg                                                           >  2:79998/1‑131 (MQ=255)
                                                                                                       ccgtcaccGGCATGCCGGCACTGATTCATGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGGCTGCTCGGTTCGCCAATGTGTTAAAGGCGCAGGGGGTAGGCGCCGGAGACCGGGTTGCCGGCtt                                        <  7:127938/141‑1 (MQ=255)
                                                                                                                     cTGGCACTGATTCATGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGGCGGCTCGGTTCGCCAATGTGTTAAAGGCGCAGGGGGTAGGCGCCGGAGACCGGGTTGCCGGCTTGATGCCGCGTAcccc                         <  4:157677/149‑1 (MQ=255)
                                                                                                                                tCATGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGGCTGCTCGGTTCGCCAATGTGTTAAAGGCGCAGGGGGTAGGCGCCGga                                                         <  1:158451/106‑1 (MQ=255)
                                                                                                                                tCATGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGGCTGCTCGGTTCGCCAATGTGTTAAAGGCGCAGGGGGTAGGCGCCGga                                                         >  2:158451/1‑106 (MQ=255)
                                                                                                                                  aTGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGGCTGCTCGGTTCGCCAATGTGTTAAAGGCGCAGGGGGTAGGCGCCGGAGACCGGGTTGCCGGCTTGATGCCGCGTACCCCTGAACTGCTGGt             >  7:247213/1‑148 (MQ=255)
                                                                                                                                  aTGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGGCTGCTCGGTTCGCCAATGTGTTAAAGGCGCAGGGGGTAGGCGCCGGAGACCGGGTTGCCGGCTTGATGCCGCGTACCCCTGAACTGCTGGt             >  7:165819/1‑148 (MQ=255)
                                                                                                                                   tGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGGCTGCTCGGTTCGCCAATGTGTTAAAGGCGCAGGGGGTAGGCGCCGGAGACCGGGTTGCCGGCTTGATGCCGCGTACCCCTGAACTGCTGGTGa           >  7:94871/1‑149 (MQ=255)
                                                                                                                                          cgcgATGGTAACAGCGCGTGCTATAGCTTCGCTCAGCTTCAGGTGCAGGCTGCTCGGTTCGCCAATGTGTTAAAGGCGCAGGGGGTAGGCGCCGGAGACCGGGTTGCCGGCTTGATGCCGCGTACCCCTGAACTGCTGGTGACCATCCt    >  1:165769/1‑149 (MQ=255)
                                                                                                                                          cgcgATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGGCTGCTCGGTTCGCCAATGTGTTAAAGGCGCAGGGGGTAGGCGCCGGAGACCGGGTTGCCGGCTTGATGCCGCGTACCCCTGAACTGCTGGTGACCATCCt    >  3:207389/1‑149 (MQ=255)
                                                                                                                                            cgATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGGCTGCTCGGTTCGCCAATGTGTTAAAGGCGCAGGGGGTAGGCGCCGGAGACCGGGTTGCCGGCTTGATGCCGCGTACCCCTGAACTGCTGGTGACCATCCTCg  >  5:179517/1‑149 (MQ=255)
                                                                                                                                                ggTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGGCTGCTCGGTTCGCCAATGTGTTAAAGGCGCAGGGGGTAGGCGCCGGAGACCGGGTTGCCGGCTTGATGc                                   <  2:139712/112‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GCCGAGGCCGCTCGTACGTTCGACCATGACCAGGCCGTTGTGACGGCATTGCACGGCAACCTTGAAGCCCTTAATGCCTGTGTCGAATGCTGTGACCGGCATGCCGGTGACGGCAAGCTGGCACTGATTCATGAGGACCGCGATGGTAACAGCGCGTGCTATAGCTTCGATCAGCTTCAGGTGCAGGCTGCTCGGTTCGCCAATGTGTTAAAGGCGCAGGGGGTAGGCGCCGGAGACCGGGTTGCCGGCTTGATGCCGCGTACCCCTGAACTGCTGGTGACCATCCTCG  >  NC_002947/2523330‑2523618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑