Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,535,933 T→C 12.5% D64G (GAC→GGC)  mrpC ← K(+)/H(+) antiporter subunit C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,535,9330TC12.5% 76.2 / 3.6 32D64G (GAC→GGC) mrpCK(+)/H(+) antiporter subunit C
Reads supporting (aligned to +/- strand):  ref base T (12/16);  new base C (2/2);  total (14/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

GTCACGCTCCCGGCCATCCACGTGGTCGGTACCGGTCAGGCCGCGCGAGGCCAGCAGCACCACCAGGAACAGCGCGGTCATGGCGAAACTGATGACGATGGCGGTGAGCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGTCCTGGGTAACGCCATCCTTGATGATTGGCTCCTTGCCGATGAACAGGCTGCCCATGCTGAAGATGAACAGGTTGACCCCGTACGAAAGCAGACACAGGCCCATGATCACCTGGTAGGTACGCGGGCGCAGGATCAGCCACACCCCGGA  >  NC_002947/2535785‑2536081
                                                                                                                                                    |                                                                                                                                                    
gTCACGCTCCCGGCCATCCACGTGGTCGGTACCGGTCAGGCCGCGCGAGGCCAGCAGCACCACCAGGAACAGCGCGGTCATGGCGAAACTGATGACGATGGCGGTGAGCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGt                                                                                                                                                      >  7:106414/1‑149 (MQ=255)
 tCACGCTCCCGGCCATCCACGTGGTCGGTACCGGTCAGGCCGCGCGAGGCCAGCAGCACCACCAGGAACAGCGCGGTCATGGCGAAACTGATGACGATGGCGGTGAGCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGTc                                                                                                                                                     >  1:263172/1‑149 (MQ=255)
   aCGCTCCCGGCCATCCACGTGGTCGGTACCGGTCAGGCCGCGCGAGGCCAGCAGCACCACCAGGAACAGCGCGGTCATGGCGAAACTGATGACGATGGCGGTGAGCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGTCCt                                                                                                                                                   <  2:66405/149‑1 (MQ=255)
     gCTCCCGGCCATCCACGTGGTCGGTACCGGTCAGGCCGCGCGAGGCCAGCAGCACCACCAGGAACAGCGCGGTCATGGCGAAACTGATGACGATGGCGGTGAGCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGTCCTgg                                                                                                                                                 >  5:208325/1‑149 (MQ=255)
           ggCCATCCACGTGGTCGGTACCGGTCAGGCCGCGCGAGGCCAGCAGCACCACCAGGAACAGCGCGGTCATGGCGAAACTGATGACGATGGCGGTGAGCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGTCCTGGGTAACg                                                                                                                                           <  3:111652/149‑1 (MQ=255)
                 ccACGTGGTCGGTACCGGTCAGGCCGCGCGAGGCCAGCAGCACCACCAGGAACAGCGCGGTCATGGCGAAACTGATGACGATGGCGGTGAGCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGTCCTGGGTAACGCCATcc                                                                                                                                     >  7:144378/1‑149 (MQ=255)
                 ccACGTGGTCGGTACCGGTCAGGCCGCGCGAGGCCAGCAGCACCACCAGGAACAGCGCGGTCATGGCGAAACTGATGACGATGGCGGTGAGCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGCCCTGGGTAACGCCATcc                                                                                                                                     >  5:43848/1‑149 (MQ=255)
                 ccACGTGGTCGGTACCGGTCAGGCCGCGCGAGGCCAGCAGCACCACCAGGAACAGCGCGGTCATGGCGAAACTGATGACGATGGCGGTGAGCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGCCCTGGGTAACGCCATcc                                                                                                                                     >  3:98608/1‑149 (MQ=255)
                  cACGTGGTCGGTACCGGTCAGGCCGCGCGAGGCCAGCAGCACCACCAGGAACAGCGCGGTCATGGCGAAACTGATGACGATGGCGGTGAGCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGTCCTGGGTAACGCCATcc                                                                                                                                     <  2:264855/148‑1 (MQ=255)
                            gTACCGGTCAGGCCGCGCGAGGCCAGCAGCACCACCAGGAACAGCGCGGTCATGGCGAAACTGATGACGATGGCTGTGAGCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGTCCTGGGTAACGCCATCCTTGATGATTgg                                                                                                                          <  5:224384/149‑1 (MQ=255)
                                   tCAGGCCGCGCGAGGCCAGCAGCACCACCAGGAACAGCGCGGTCATGGCGAAACTGATGACGATGGCGGTGAGCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGTCCTGGGTAACGCCATCCTTGATGATTGGCTCCTTg                                                                                                                   <  2:143163/149‑1 (MQ=255)
                                             cgAGGCCAGCAGCACCACCAGGAACAGCGCGGTCATGGCGAAACTGATGACGATGGCGGTGAGCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGTCCTGGGTAACGCCATCCTTGATGATTGGCTCCTTGCCGATGAACa                                                                                                         <  8:144378/149‑1 (MQ=255)
                                                ggCCAGCAGCACCACCAGGAACAGCGCGGTCATGGCGAAACTGATGACGATGGCGGTGAGCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGTCCTGGGTAACGCCATCCTTGATGATTGGCTCCTTGCCGATGAACAGGc                                                                                                      <  2:87893/149‑1 (MQ=255)
                                                        gcaCCACCAGGAACAGCGCGGTCATGGCGAAACTGATGACGATGGCGGTGAGCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGTCCTGGGTAACGCCATCCTTGATGATTGGCTCCTTGCCGATGAACAGGCTGCCCATg                                                                                              >  7:76028/1‑149 (MQ=255)
                                                            caccaGGAACAGCGCGGTCATGGCGAAACTGATGACGATGGCGGTGAGCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGTCCTGGGTAACGCCATCCTTGATGATTGGCTCCTTGCCGATGAACAGGCTGCCCATGCTg                                                                                           >  8:247059/1‑148 (MQ=255)
                                                            caccaGGAACAGCGCGGTCATGGCGAAACTGATGACGATGGCGGTGAGCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGTCCTGGGTAACGCCATCCTTGATGATTGGCTCCTTGCCGATGAACAGGCTGCCCATGCTg                                                                                           >  2:201234/1‑148 (MQ=255)
                                                            caccaGGAACAGCGCGGTCATGGCGAAACTGATGACGATGGCGGTGAGCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGTCCTGGGTAACGCCATCCTTGATGATTGGCTCCTTGCCGATGAACAGGCTGCCCATGCTGa                                                                                          <  6:208325/149‑1 (MQ=255)
                                                              ccaGGAACAGCGCGGTCATGGCGTAACTGATGACGATGGCGGTGAGCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGTCCTGGGTAACGCCATCCTTGATGATTGGGTCCTTGCCGATGAACAGGCTGCCCATGCTGAAg                                                                                        <  5:92039/149‑1 (MQ=255)
                                                                    aCAGCGCGGTCATGGCGAAACTGATGACGATGGCGGTGAGCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGCCCTGGGTAACGCCATCCTTGATGATTGGCTCCTTGCCGATGAACAGGCTGCCCATGCTGAAGATGAAc                                                                                  <  6:43848/149‑1 (MQ=255)
                                                                    aCAGCGCGGTCATGGCGAAACTGATGACGATGGCGGTGAGCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGCCCTGGGTAACGCCATCCTTGATGATTGGCTCCTTGCCGATGAACAGGCTGCCCATGCTGAAGATGAAc                                                                                  <  4:98608/149‑1 (MQ=255)
                                                                         gcgGTCATGGCGAAACTGATGACGATGGCGGTGAGCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGTCCTGGGTAACGCCATCCTTGATGATTGGCTCCTTGCCGATGAACAGGCTGCCCATGCTGAAGATGAACAGGtt                                                                             <  8:76028/149‑1 (MQ=255)
                                                                                         tgatgaCGATGGCGGTGAGCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGTCCTGGGTAACGCCATCCTTGATGATTGGCTCCTTGCCGATGAACAGGCTGCCCATGCTGAAGATGAACAGGTTGACCCCGTACGAAAg                                                              >  3:12125/1‑148 (MQ=255)
                                                                                                       gTGAGCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGTCCTGGGTAACGCCATCCTTGATGATTGGCTCCTTGCCGATGAACAGGCTGCCCATGCTGAAGATGAACAGGTTGACCCCGTACGAAAGCAGACACAGGCCCAt                                               >  1:146438/1‑149 (MQ=255)
                                                                                                        tGAGCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGTCCTGGGTAACGCCATCCTTGATGATTGGCTCCTTGCCGATGAACAGGCTGCCCATGCTGAAGATGAACAGGTTGACCCCGTACGAAAGCAGACACAGGCCCATg                                              <  2:245557/149‑1 (MQ=255)
                                                                                                           gCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGTCCTGGGTAACGCCATCCTTGATGATTGGCTCCTTGCCGATGAACAGGCTGCCCATGCTGAAGATGAACAGGTTGACCCCGTACGAAAGCAGACACAGGCCCATGAt                                            <  7:247059/148‑1 (MQ=255)
                                                                                                                aGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGTCCTGGGTAACGCCATCCTTGATGATTGGCTCCTTGCCGATGAACAGGCTGCCCATGCTGAAGATGAACAGGTTGACCCCGTACGAAAGCAGACACAGGCCCATGATCACCTg                                      <  2:146438/149‑1 (MQ=255)
                                                                                                                        tgGCAGCGGGTCGGTGTAATGCAGCAGGTCCTGGGTAACGCCATCCTTGATGATTGGCTCCTTGCCGATGAACAGGCTGCCCATGCTGAAGATGAACAGGTTGACCCCGTACGAAAGCAGACACAGGCCCATGATCACCTggtaggta                               <  3:113811/148‑1 (MQ=255)
                                                                                                                            aGCGGGTCGGTGTAAAGCAGCAGGTCCTGGGTAACGCCATCCTTGATGATTGGCTCCTTGCCGATGAACAGGCTGCCCATGCTGAAGATGAACAGGTTGACCCCGTACGAAAGCAGACACAGGCCCATGATCACCTGGTAGGTACGCgg                          >  7:230901/1‑149 (MQ=255)
                                                                                                                             gCGGGTCGGTGTAATGCAGCAGGTCCTGGGTAACGCCATCCTTGATGATTGGCTCCTTGCCGATGAACAGGCTGCCCATGCTGAAGATGAACAGGTTGACCCCGTACGAAAGCAGACACAGGCCCATGATCACCTGGTAGGTACGCggg                         >  8:24880/1‑149 (MQ=255)
                                                                                                                             gCGGGTCGGTGTAATGCAGCAGGTCCTGGGTAACGCCATCCTTGATGATTGGCTCCTTGCCGATGAACAGGCTGCCCATCCTGAAGATGAACAGGTTGACCCCGTACGAGAGCAGACACAGGCCCATGATCACCTGGTAGGTACGCggg                         >  8:77328/1‑149 (MQ=255)
                                                                                                                                           tGCAGCAGGTCCTGGGTAACGCCATCCTTGATGATTGGCTCCTTGCCGATGAACAGGCTGCCCATGCTGAAGATGAACAGGTTGACCCCGTACGAAAGCAGACACAGGCCCATGATCACCTGGTAGGTACGCGGGCGCAGGATCAGCca           <  2:263172/149‑1 (MQ=255)
                                                                                                                                                  ggTCCTGGGTAACGCCATCCTTGATGATTGGCTCCTTGCCGATGAACAGGCTGCCCATGCTGAAGATGAACAGGTTGACCCCGTACGAAAGCAGACACAGGCCCATGATCACCTGGTAGGTACGCGGgc                        <  2:62723/129‑1 (MQ=255)
                                                                                                                                                  ggTCCTGGGTAACGCCATCCTTGATGATTGGCTCCTTGCCGATGAACAGGCTGCCCATGCTGAAGATGAACAGGTTGACCCCGTACGAAAGCAGACACAGGCCCATGATCACCTGGTAGGTACGCGGgc                        >  1:62723/1‑129 (MQ=255)
                                                                                                                                                    tCCTGGGTAACGCCATCCTTGATGATTGGCTCCTTGCCGATGAACAGGCTGCCCATGCTGAAGATGAACAGGTTGACCCCGTACGAAAGCAGACACAGGCCCATGATCACCTGGTAGGTACGCGGGCGCAGGATCAGCCACACCCCGGa  <  4:12125/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTCACGCTCCCGGCCATCCACGTGGTCGGTACCGGTCAGGCCGCGCGAGGCCAGCAGCACCACCAGGAACAGCGCGGTCATGGCGAAACTGATGACGATGGCGGTGAGCACCAGTGCCTGTGGCAGCGGGTCGGTGTAATGCAGCAGGTCCTGGGTAACGCCATCCTTGATGATTGGCTCCTTGCCGATGAACAGGCTGCCCATGCTGAAGATGAACAGGTTGACCCCGTACGAAAGCAGACACAGGCCCATGATCACCTGGTAGGTACGCGGGCGCAGGATCAGCCACACCCCGGA  >  NC_002947/2535785‑2536081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑