Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,555,170 A→G 11.1% Q384R (CAG→CGG)  PP_2240 → ABC transporter permease/ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,555,1700AG11.1% 143.1 / 7.3 54Q384R (CAG→CGG) PP_2240ABC transporter permease/ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (25/23);  new base G (3/3);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGTGTTTCCGTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCCGGACGGACATGCCC  >  NC_002947/2555028‑2555313
                                                                                                                                              |                                                                                                                                               
gttgtGTTTCCGTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACcg                                                                                                                                           >  5:40845/1‑149 (MQ=255)
    tgtTTCCGTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACcgcgc                                                                                                                                        >  3:252867/1‑148 (MQ=255)
    tgtTTCCGTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTCCCCAGGCCTTTGGGCAGGTGCCGGATGCTCTGTCGTTCTTCCGTGAGTCGTCCGATGCGTGCGCGCGGTACtgcgc                                                                                                                                        <  2:145432/148‑1 (MQ=255)
     gtTTCCGTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGa                                                                                                                                      >  6:261567/1‑149 (MQ=255)
      tttCCGTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGAc                                                                                                                                     >  4:232506/1‑149 (MQ=255)
           gTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGAccc                                                                                                                                   >  1:238750/1‑146 (MQ=255)
           gTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGAccc                                                                                                                                   <  2:238750/146‑1 (MQ=255)
           gTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTg                                                                                                                                <  2:160732/149‑1 (MQ=255)
                  cttcAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGtc                                                                                                                          <  7:49886/148‑1 (MQ=255)
                       ggCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCg                                                                                                                    >  3:67163/1‑149 (MQ=255)
                          gccgcGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCt                                                                                                                 <  3:232506/149‑1 (MQ=255)
                                   cttcAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGa                                                                                                          <  6:35438/147‑1 (MQ=255)
                                   cttcAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGAt                                                                                                         <  6:40845/148‑1 (MQ=255)
                                     tcAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGc                                                                                                       >  3:251059/1‑148 (MQ=255)
                                       aGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCaa                                                                                                    <  6:8517/149‑1 (MQ=255)
                                           gggCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACca                                                                                                 <  4:88701/148‑1 (MQ=255)
                                                    aGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGt                                                                                       <  4:252867/149‑1 (MQ=255)
                                                          gCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTg                                                                                 >  6:48867/1‑149 (MQ=255)
                                                           cGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCt                                                                                                                 <  7:79304/116‑1 (MQ=255)
                                                           cGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCt                                                                                                                 >  8:79304/1‑116 (MQ=255)
                                                           cGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGc                                                                                >  8:590/1‑149 (MQ=255)
                                                            gACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTg                                                                                                                                <  5:242071/100‑1 (MQ=255)
                                                            gACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTg                                                                                                                                >  6:242071/1‑100 (MQ=255)
                                                                tCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCcgcgc                                                                           <  1:96511/149‑1 (MQ=255)
                                                                tCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCcgcgc                                                                           >  2:96511/1‑149 (MQ=255)
                                                                    gCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTca                                                                       >  7:141058/1‑149 (MQ=255)
                                                                      aaaCTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGt                                                                                       <  1:197196/131‑1 (MQ=255)
                                                                      aaaCTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGt                                                                                       >  2:197196/1‑131 (MQ=255)
                                                                      aaaCTTCCCAGGCCTTTGGGC‑GGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGc                                                                                <  1:43177/137‑1 (MQ=255)
                                                                      aaaCTTCCCAGGCCTTTGGGC‑GGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGc                                                                                >  2:43177/1‑137 (MQ=255)
                                                                               aGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGAc                                                            <  7:590/149‑1 (MQ=255)
                                                                               aGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGAc                                                            <  4:67163/149‑1 (MQ=255)
                                                                               aGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGAc                                                            >  6:68007/1‑149 (MQ=255)
                                                                               aGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTACATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCCAAGAc                                                            >  6:158271/1‑149 (MQ=255)
                                                                                       gggCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCa                                                    >  4:141645/1‑149 (MQ=255)
                                                                                                cAGGATTCTCTGTCGTTCTTCCGTAAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTg                                             >  6:24289/1‑147 (MQ=255)
                                                                                                        tctGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACgg                                   >  5:107364/1‑149 (MQ=255)
                                                                                                          tGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACg                                    <  5:261567/146‑1 (MQ=255)
                                                                                                                    ccGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGtgc                       >  8:152069/1‑149 (MQ=255)
                                                                                                                          gTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTgcgc                  <  5:158271/148‑1 (MQ=255)
                                                                                                                              tACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCcgga             <  7:152069/149‑1 (MQ=255)
                                                                                                                                cGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCcggac            <  5:68007/148‑1 (MQ=255)
                                                                                                                                 gATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTg                                             >  5:51434/1‑114 (MQ=255)
                                                                                                                                 gATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTg                                             <  6:51434/114‑1 (MQ=255)
                                                                                                                                      ggtCGCGCGGTACTGCGCGAACCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCcgga             <  3:105704/139‑1 (MQ=255)
                                                                                                                                      ggtCGCGCGGTACTGCGCGAACCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCcgga             >  4:105704/3‑141 (MQ=255)
                                                                                                                                      gTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCCGGACGGACATg     >  8:238284/1‑149 (MQ=255)
                                                                                                                                       gtCGCGCGGTACTGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCCGGACGGACATGc    >  8:179748/2‑149 (MQ=255)
                                                                                                                                       gtCGCGCGGTACTGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCCGGACGGACATGc    >  4:156683/2‑149 (MQ=255)
                                                                                                                                          gcgcGGTACTGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCCGGACGGACATGccc  <  3:156683/148‑1 (MQ=255)
                                                                                                                                             cAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACg                                    <  4:43262/111‑1 (MQ=255)
                                                                                                                                             cAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACg                                    >  3:43262/1‑111 (MQ=255)
                                                                                                                                             cAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCCGGACGGACATGc    <  3:63174/143‑1 (MQ=255)
                                                                                                                                             cAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCCGGACGGACATGc    >  4:63174/1‑143 (MQ=255)
                                                                                                                                              |                                                                                                                                               
GTTGTGTTTCCGTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCCGGACGGACATGCCC  >  NC_002947/2555028‑2555313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑