Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,555,175 C→T 9.2% R386C (CGC→TGC)  PP_2240 → ABC transporter permease/ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,555,1750CT 9.2% 149.8 / 4.9 54R386C (CGC→TGC) PP_2240ABC transporter permease/ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (24/25);  new base T (3/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

GTTGTGTTTCCGTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCCGGACGGACATGCCCTTATCGCC  >  NC_002947/2555028‑2555321
                                                                                                                                                   |                                                                                                                                                  
gttgtGTTTCCGTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACcg                                                                                                                                                   >  5:40845/1‑149 (MQ=255)
    tgtTTCCGTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACcgcgc                                                                                                                                                >  3:252867/1‑148 (MQ=255)
    tgtTTCCGTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTCCCCAGGCCTTTGGGCAGGTGCCGGATGCTCTGTCGTTCTTCCGTGAGTCGTCCGATGCGTGCGCGCGGTACtgcgc                                                                                                                                                <  2:145432/148‑1 (MQ=255)
     gtTTCCGTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGa                                                                                                                                              >  6:261567/1‑149 (MQ=255)
      tttCCGTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGAc                                                                                                                                             >  4:232506/1‑149 (MQ=255)
           gTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGAccc                                                                                                                                           >  1:238750/1‑146 (MQ=255)
           gTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGAccc                                                                                                                                           <  2:238750/146‑1 (MQ=255)
           gTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTg                                                                                                                                        <  2:160732/149‑1 (MQ=255)
                  cttcAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGtc                                                                                                                                  <  7:49886/148‑1 (MQ=255)
                       ggCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCg                                                                                                                            >  3:67163/1‑149 (MQ=255)
                          gccgcGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCt                                                                                                                         <  3:232506/149‑1 (MQ=255)
                                   cttcAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGa                                                                                                                  <  6:35438/147‑1 (MQ=255)
                                   cttcAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGAt                                                                                                                 <  6:40845/148‑1 (MQ=255)
                                     tcAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGc                                                                                                               >  3:251059/1‑148 (MQ=255)
                                       aGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCaa                                                                                                            <  6:8517/149‑1 (MQ=255)
                                           gggCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACca                                                                                                         <  4:88701/148‑1 (MQ=255)
                                                    aGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGt                                                                                               <  4:252867/149‑1 (MQ=255)
                                                          gCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTg                                                                                         >  6:48867/1‑149 (MQ=255)
                                                           cGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCt                                                                                                                         <  7:79304/116‑1 (MQ=255)
                                                           cGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCt                                                                                                                         >  8:79304/1‑116 (MQ=255)
                                                           cGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGc                                                                                        >  8:590/1‑149 (MQ=255)
                                                            gACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTg                                                                                                                                        <  5:242071/100‑1 (MQ=255)
                                                            gACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTg                                                                                                                                        >  6:242071/1‑100 (MQ=255)
                                                                tCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCcgcgc                                                                                   <  1:96511/149‑1 (MQ=255)
                                                                tCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCcgcgc                                                                                   >  2:96511/1‑149 (MQ=255)
                                                                    gCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTca                                                                               >  7:141058/1‑149 (MQ=255)
                                                                      aaaCTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGt                                                                                               <  1:197196/131‑1 (MQ=255)
                                                                      aaaCTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGt                                                                                               >  2:197196/1‑131 (MQ=255)
                                                                      aaaCTTCCCAGGCCTTTGGGC‑GGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGc                                                                                        <  1:43177/137‑1 (MQ=255)
                                                                      aaaCTTCCCAGGCCTTTGGGC‑GGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGc                                                                                        >  2:43177/1‑137 (MQ=255)
                                                                               aGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGAc                                                                    <  7:590/149‑1 (MQ=255)
                                                                               aGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGAc                                                                    <  4:67163/149‑1 (MQ=255)
                                                                               aGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGAc                                                                    >  6:68007/1‑149 (MQ=255)
                                                                               aGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTACATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCCAAGAc                                                                    >  6:158271/1‑149 (MQ=255)
                                                                                       gggCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCa                                                            >  4:141645/1‑149 (MQ=255)
                                                                                                cAGGATTCTCTGTCGTTCTTCCGTAAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTg                                                     >  6:24289/1‑147 (MQ=255)
                                                                                                        tctGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACgg                                           >  5:107364/1‑149 (MQ=255)
                                                                                                          tGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACg                                            <  5:261567/146‑1 (MQ=255)
                                                                                                                    ccGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGtgc                               >  8:152069/1‑149 (MQ=255)
                                                                                                                          gTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTgcgc                          <  5:158271/148‑1 (MQ=255)
                                                                                                                              tACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCcgga                     <  7:152069/149‑1 (MQ=255)
                                                                                                                                cGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCcggac                    <  5:68007/148‑1 (MQ=255)
                                                                                                                                 gATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTg                                                     <  6:51434/114‑1 (MQ=255)
                                                                                                                                 gATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTg                                                     >  5:51434/1‑114 (MQ=255)
                                                                                                                                      ggtCGCGCGGTACTGCGCGAACCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCcgga                     <  3:105704/139‑1 (MQ=255)
                                                                                                                                      ggtCGCGCGGTACTGCGCGAACCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCcgga                     >  4:105704/3‑141 (MQ=255)
                                                                                                                                      gTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCCGGACGGACATg             >  8:238284/1‑149 (MQ=255)
                                                                                                                                       gtCGCGCGGTACTGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCCGGACGGACATGc            >  8:179748/2‑149 (MQ=255)
                                                                                                                                       gtCGCGCGGTACTGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCCGGACGGACATGc            >  4:156683/2‑149 (MQ=255)
                                                                                                                                          gcgcGGTACTGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCCGGACGGACATGccc          <  3:156683/148‑1 (MQ=255)
                                                                                                                                             cAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACg                                            >  3:43262/1‑111 (MQ=255)
                                                                                                                                             cAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACg                                            <  4:43262/111‑1 (MQ=255)
                                                                                                                                             cAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCCGGACGGACATGc            <  3:63174/143‑1 (MQ=255)
                                                                                                                                             cAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCCGGACGGACATGc            >  4:63174/1‑143 (MQ=255)
                                                                                                                                               gTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCCGGACGGACATGCCCTTATCg    <  4:251059/149‑1 (MQ=255)
                                                                                                                                                tACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCCGGACGGACATGCCCTTATCGc   <  6:107364/149‑1 (MQ=255)
                                                                                                                                                 aCCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCCGGACGGACATGCCCTTATCGcc  >  6:27007/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTTGTGTTTCCGTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCCGGACGGACATGCCCTTATCGCC  >  NC_002947/2555028‑2555321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑