| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,584,611 | C→T | 8.3% | intergenic (+98/+29) | PP_2264 → / ← folD‑II | sugar ABC transporter substrate‑binding protein/bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,584,611 | 0 | C | T | 8.3% | 134.2 / 2.5 | 48 | intergenic (+98/+29) | PP_2264/folD‑II | sugar ABC transporter substrate‑binding protein/bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase |
| Reads supporting (aligned to +/- strand): ref base C (20/24); new base T (2/2); total (22/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.76e-01 | |||||||||||
ACAACGAACTGCTGAAGTCCTGGGAGGCGGCCAGGAAGTGATCGGCTGAACGCCGGGGTGGTTTCACCGACCGCGGGGGCCGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGCGTGTTCTCCAGCAGGCACGCCCGGGTCATCGGCCCGACGCCGCCCGGCACCGGTGTGATCCAGCCGGCGCGGGGCAGGGCAGTCTCG > NC_002947/2584473‑2584757 | aCAACGAACTGCTGAAGTCCTGGGAGGCGGCCAGGAAGTGATCGGCTGAACGCCGGGGTGGTTTCACCGACCGCGGGGGCCGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCg > 8:127050/1‑149 (MQ=255) aaCGAACTGCTGAAGTCCTGGGAGGCGGCCAGGAAGTGATCGGCTGAACGCCGGGGTGGTTTCACCGACCGCGGGGGCCGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTTTGCAGGT‑GCCGtt > 1:201191/1‑149 (MQ=255) cGAACTGCTGAAGTCCTGGGAGGCGGCCAGGAAGTGATCGGCTGAACGCCGGGGTGGTTTCACCGACCGCGGGGGCCGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTTTGCAGGT‑GCCGtttt < 2:201191/149‑1 (MQ=255) cGAAATGCTGAAGTGCTGGGAGGCGGCCAGGAAGTGATCGTCTGAACGCCGGGGTGGTTTCACCGACCGCGGGGGCCGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAATGT‑GCCGtttt < 8:142218/149‑1 (MQ=255) gacctgctgAAGTCCTGGGAGGCGGCCAGGAAGTGCTCGGCTGAACGCCGGGGTGGTTTCACCGACCGCGGGGGCCGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTc < 2:117657/146‑1 (MQ=255) gAACTGCTGAAGTCCTGGGAGGCGGCCAGGAAGTGATCGGCTGAACGCCGGCGTGGTTTCACCGACCGCGGGGGCCGTCCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAAAGGCACCTGCAAACGT‑GACGTTTTc > 7:130274/1‑149 (MQ=255) tgAAGTCCTGGGAGGCGGCCAGGAAGTGATCGGCTGAACGCCGGGGTGGTTTCACCGACCGCGGGGGCCGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCAc > 8:215755/1‑149 (MQ=255) aaGTCCTGGGAGGCGGCCAGGAAGTGATCGGCTGAACGCCGGGGTGGTTTCACCGACCGCGGGGGCCGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCAc < 1:88593/147‑1 (MQ=255) aGTCCTGGGAGGCGGCCAGGAAGTGATCGGCTGAACGCCGGGGTGGTTTCACCGACCGCGGGGGCCGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCa < 7:81243/149‑1 (MQ=255) cTGGGAGGCGGCCAGGAAGTGATCGGCTGAACGCCGGGGTGGTTTCACCGACCGCGGGGGCCGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTAt > 5:167934/1‑149 (MQ=255) ggAGGCGGCCAGGAAGTGATCGGCTGAACGCCGGGGTGGTTTCACCGACCGCGGGGGCCGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTAtt < 7:127050/147‑1 (MQ=255) gcggaCAGGAAGTGATCGGCTGAAGGCCGGGGTGGTTTCACCGACCGCGGGGGCCGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTtg < 8:130274/148‑1 (MQ=255) cggcCAGGAAGTGATCGGCTGAACGCCGGGGTGGTTTCACCGACCGCGGGGGCCGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTtgtg < 6:167934/149‑1 (MQ=255) gAAGTGATCGGCTGAACGCCGGGGTGGTTTCACCGACCGCGGGGGCCGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGctct > 2:191515/1‑149 (MQ=255) cGGCTGAACGCCGGGGTGGTTTCACCGACCGCGGGGGCCGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCg < 3:209085/148‑1 (MQ=255) ggCTGAACGCCGGGGTGGTTTCACCGACCGCGGGGGCCGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGTCTCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCg < 1:191515/148‑1 (MQ=255) ggCTGAACGCCGGGGTGGTTTCACCGACCGCGGGGGCCGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCTGCa < 7:215755/149‑1 (MQ=255) gAACGCCGGGGTGGTTTCACCGACCGCGGGGGCCGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACa > 4:127655/1‑149 (MQ=255) gggTGGTTTCACCGACCGCGGGGGCCGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGCGTGTtc < 8:122688/149‑1 (MQ=255) cgaccgCGGGGGCCGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGCGTGTTCTCCAGCAGGCAc > 3:72155/1‑149 (MQ=255) gcgGGGGCCGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGCGTGTTCTCCAGCAGGCAc > 4:179936/1‑144 (MQ=255) gcgGGGGCCGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGCGTGTTCTCCAGCAGGCAc < 3:179936/144‑1 (MQ=255) cgGGGGCCGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGc < 3:57080/125‑1 (MQ=255) cgGGGGCCGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGc > 4:57080/1‑125 (MQ=255) gggggCCGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGCGTGTTCTCCAGCAGGCACGCCCggg < 4:72155/149‑1 (MQ=255) ccGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGCGTGTTCTCCAGCAGGCACGCCCGGGTCATc < 3:127655/149‑1 (MQ=255) gCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGCGTGTTCTCCAGCAGGCACGCCCGGGTCATCGGcc > 3:52276/1‑149 (MQ=255) cAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCAt < 8:95187/109‑1 (MQ=255) cAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCAt > 7:95187/1‑109 (MQ=255) tcgctcgcGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGCGTGTTCTCCAGCAGGCACGCCCGGGTCATCGGCCCGAcgccg > 5:174957/1‑149 (MQ=255) gctcgcGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGCGTGTTCTCCAGCAGGCACGCCCGGGTCATCGGCCCGACgccgcc < 6:174957/149‑1 (MQ=255) tcgcGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GGCGGTTTAGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGCGTGTTCTCCAGCAGGCACGCCCGGGTCATCGGCCCCACGCCGCCCg > 5:189946/1‑149 (MQ=255) tcgcGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGCGTGTtctc > 2:268576/1‑111 (MQ=255) tcgcGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGCGTGTtctc < 1:268576/111‑1 (MQ=255) cTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGCGTGTTCTCCAGCAGGCACGCCCGGGTCATCGGCCCGACGCCGCCCGGCACCGGTGTGATc < 1:161507/149‑1 (MQ=255) ggCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGCGTGTTCTCCAGCAGGCACGCCCGGGTCATCGGCCCGACGCCGCCCGGCACCGGTGTGATCCAGCCGgcg > 8:62673/1‑149 (MQ=255) gCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTAt < 2:253609/49‑1 (MQ=255) gCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTAt > 1:253609/1‑49 (MQ=255) cAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGCGTGTTCTCCAGCAGGCACGCCCGGGTCATCGGCCCGACGCCGCCCGGCACCGGTGTGATCCAGCCGGcgcg < 3:127105/149‑1 (MQ=255) aaaaaaaaCGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGAGTGTTCTCCAGCAGGCACGCCCGGGTCATCGGCCCGACGCCGCCCGGCACCGGTGTGATCCAGCCGGCACgg > 1:53699/1‑149 (MQ=255) gcgaaaaCGGCACCTTTGCAGGT‑GCCGTTTTCTCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGCGTGTTCTCca < 5:274677/83‑1 (MQ=25) gcgaaaaCGGCACCTTTGCAGGT‑GCCGTTTTCCCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGCGTGTTCTCca > 6:274677/4‑86 (MQ=25) aaaaaaCGGCACCTTCATAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGCGTGTTCTCCAGCAGGCACGCCCGGGTCATCGGCCCGACGCCGCCCGGCACCGGTGTGATCCAGCCGGCGCgggg < 6:189946/149‑1 (MQ=255) ggCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGCGTGTTCTCCAGCAGGCACGCCCGGGTCATCGGCCCGACGCCGCCCGGCACCGGTGTGATCCAGCCGGCGCGgggcagggca < 5:141081/149‑1 (MQ=255) aCCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGCGTGTTCTCCAGCAGGCACGCCCGGGTCATCGGCCCGACGCCGCCCGGCACCGGTGTGATCCa > 5:171452/1‑127 (MQ=255) aCCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGCGTGTTCTCCAGCAGGCACGCCCGGGTCATCGGCCCGACGCCGCCCGGCACCGGTGTGATCCa < 6:171452/127‑1 (MQ=255) cTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGCGTGTTCTCCAGCAGGCACGCCCGGGTCATCGGCCCGACGCCGCCCGGCACCGGTGTGATCCAGCCGGCGCGGGGCAGGGCAGtctc > 8:10616/1‑149 (MQ=255) gCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGCGTGTTCTCCAGCAGGCACGCCCGGGTCATCGGCCCGACGCCGCCCGGCACCGGTGTGATCCAGCCGGCGCGGGGCAGGGCAGTCTCg > 7:51843/1‑148 (MQ=255) | ACAACGAACTGCTGAAGTCCTGGGAGGCGGCCAGGAAGTGATCGGCTGAACGCCGGGGTGGTTTCACCGACCGCGGGGGCCGTGCAACCGCTCGCTCGCGAAGTGGCCACTAGTCACGGGCAAAAAAAACGGCACCTGCAAAGGT‑GCCGTTTTCGCATCACGCAGTATTACTTGTGCAGCTCTTCTGCCGCATACAGCGTGTTCTCCAGCAGGCACGCCCGGGTCATCGGCCCGACGCCGCCCGGCACCGGTGTGATCCAGCCGGCGCGGGGCAGGGCAGTCTCG > NC_002947/2584473‑2584757 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |