Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 262,948 G→C 11.8% intergenic (+183/‑302) PP_0211 → / → gabD‑I hypothetical protein/succinate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947262,9480GC11.8% 135.0 / 9.7 51intergenic (+183/‑302)PP_0211/gabD‑Ihypothetical protein/succinate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (24/21);  new base C (3/3);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

GCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCTCCCACAACACAAAAAAGT  >  NC_002947/262801‑263096
                                                                                                                                                   |                                                                                                                                                    
gCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGc                                                                                                                                                     >  4:60580/1‑149 (MQ=255)
      cgacCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTgct                                                                                                                                                >  8:82408/1‑148 (MQ=255)
         ccGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTgctgagc                                                                                                                                            <  3:60580/149‑1 (MQ=255)
         ccGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGCCCTGATGCCGTgctgaaa                                                                                                                                            <  3:51751/149‑3 (MQ=255)
         ccGCCTAGCCAACGAACTGGCCGAGGGGAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGCCCTGATGCCGTgctgaaa                                                                                                                                            <  5:277553/149‑3 (MQ=255)
          cGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGCCCTGATGCCGTgctgaaac                                                                                                                                           <  5:76230/149‑4 (MQ=255)
            ccTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTTCTGCGCGCAACTGGGCAACGTGCCACCGATTACTACGCCGATGACTACAGCATCATCGTTTCAGAAGGGCATCAGGGCGTgctgaggtga                                                                                                                                         <  2:229213/149‑1 (MQ=255)
                                  ggTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTGAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCt                                                                                                                    <  2:265211/148‑1 (MQ=255)
                                       ccTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCa                                                                                                              <  7:82408/149‑1 (MQ=255)
                                        cTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGTGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCa                                                                                                              >  6:95003/1‑148 (MQ=255)
                                        cTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCAt                                                                                                             <  6:10935/149‑1 (MQ=255)
                                              tttGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTc                                                                                                                     >  1:1326/1‑135 (MQ=255)
                                              tttGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTc                                                                                                                     <  2:1326/135‑1 (MQ=255)
                                                   cGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGa                                                                                                  <  5:110345/149‑1 (MQ=255)
                                                     acacCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAgg                                                                                                >  8:208553/1‑149 (MQ=255)
                                                       cccccGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAggg                                                                                               <  4:261241/147‑1 (MQ=255)
                                                        ccccGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGAAGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGcc                                                                                             >  3:199653/1‑149 (MQ=255)
                                                          ccGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGt                                                                                           >  5:9511/1‑149 (MQ=255)
                                                          ccGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGt                                                                                           >  6:179049/1‑149 (MQ=255)
                                                          ccGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGt                                                                                           <  2:62019/149‑1 (MQ=255)
                                                                   ggTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAgg                                                                                                >  1:60181/1‑135 (MQ=255)
                                                                   ggTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAgg                                                                                                <  2:60181/135‑1 (MQ=255)
                                                                   ggTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTcccc                                                                                   <  4:227211/148‑1 (MQ=255)
                                                                           gcgcAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACAt                                                                          >  4:138212/1‑149 (MQ=255)
                                                                               aaCTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACAtttct                                                                      >  4:124742/1‑149 (MQ=255)
                                                                               aaCTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACAtttct                                                                      >  7:205315/1‑149 (MQ=255)
                                                                                aCTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACAtttctt                                                                     >  2:234792/1‑149 (MQ=255)
                                                                                 cTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACAtttcttt                                                                    <  3:124742/149‑1 (MQ=255)
                                                                                       aaagagCCACATATTACGATCCCGATGAAAACAGCATCATCAATTCAGCAAGGCAAAAGGCCGTCCTGACCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCATCCTGGAGGGCCGTCGGTCCACCCTTCACATTTCTATCAGtt                                                               >  8:180914/6‑148 (MQ=255)
                                                                                           tGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTc                                                          <  8:205315/149‑1 (MQ=255)
                                                                                             ccACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGt                                                        >  3:252057/1‑149 (MQ=255)
                                                                                             ccACCGATTACGATGCCGATGACAACACCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTc                                                                                                       >  8:211903/1‑102 (MQ=255)
                                                                                             ccACCGATTACGATGCCGATGACAACACCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTc                                                                                                       <  7:211903/102‑1 (MQ=255)
                                                                                                 cGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTaa                                                    <  5:179049/149‑1 (MQ=255)
                                                                                                  gATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAAc                                                   <  3:138212/149‑1 (MQ=255)
                                                                                                   aTTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTTCCCCATTCACATTTCTTTCAGTTCATTCGTCTAAcc                                                  >  7:93520/1‑149 (MQ=255)
                                                                                                     gatgATGCTGTTGTCATCGGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTg                                                >  5:8043/4‑149 (MQ=255)
                                                                                                       cGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTa                                              >  5:70997/1‑149 (MQ=255)
                                                                                                           gCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTgg                                          >  4:129115/1‑149 (MQ=255)
                                                                                                            ccGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGc                                         <  6:70997/149‑1 (MQ=255)
                                                                                                              gATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGccc                                       <  5:95003/149‑1 (MQ=255)
                                                                                                              gATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGccc                                       >  6:215281/1‑149 (MQ=255)
                                                                                                                      caGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACATCTCCAAGGCGTTCTGATTAATCCCCACCGGGAGGGCCGTCGGTCCCCCCTACACATTAATTTAAGTTCATTCGTGTAACATGTAGTGGCAATATCGCTg                               >  1:149859/1‑149 (MQ=255)
                                                                                                                         catcatCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCa                            <  1:234792/149‑1 (MQ=255)
                                                                                                                              tcagctCAGCACGCCCTGATGCCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCa                       >  8:47486/6‑149 (MQ=255)
                                                                                                                                gTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGc                     >  6:162890/1‑149 (MQ=255)
                                                                                                                                  ctCAGCACGCCCTGATGCCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCACGCCAGCTc                   >  8:91351/2‑149 (MQ=255)
                                                                                                                                   tCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGATATGATTAATCACCTCCGGGAGGGCCGTCGGTCACCCCTTAACATTTCTTTCAGTTAATTCGTCTAACCTGTACTGGCCCTATAGCTGGCAAGCCAGCTcc                  >  7:168767/1‑149 (MQ=255)
                                                                                                                                     aGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCTCCca                <  5:215281/149‑1 (MQ=255)
                                                                                                                                       cACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCTCCcac               >  6:76517/1‑148 (MQ=255)
                                                                                                                                         cGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCTCCcacaac            <  7:208553/149‑1 (MQ=255)
                                                                                                                                                   gCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCTCCCACAACACAAAAAAGt  <  6:8043/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGTGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCTCCCACAACACAAAAAAGT  >  NC_002947/262801‑263096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑