| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,593,944 | C→T | 13.7% | R10C (CGC→TGC) | PP_5510 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,593,944 | 0 | C | T | 13.7% | 66.6 / 3.7 | 30 | R10C (CGC→TGC) | PP_5510 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (11/14); new base T (2/2); total (14/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01 | |||||||||||
AGCGACCACCGTGGCTCCGCGTCCGGCCTATGTGGCTCCACGCCCAGCCTACGTTGCACCGCGAGCCAGCTACAG‑CTATCGCACCAGCAGCTTCTCCAGCTTCCGCTCCCGTAGGTAAGCCATGACGACTCCAGCCCAACGGCCTCCGCGCCTGAAAGAACTGTCCTTGCGCATTGAGCGTGTGGACAACGGAGTCATCGTCCGGTCCCGAGACAACATCCTGGGCCTGGCAAACGAAGGTGACTGCAACGTGTTCACCAATCGCTACGAACTGAGCAACTACCTCAACGCTT > NC_002947/2593796‑2594088 | accGACCCCCGTGCCTCCGCGGCCGGCCTATGTGGCCCCAAGCCCAGCCTACGTTCCACCGCGAGCCACCTACCG‑CTATCGCACCAGCAGCTTCTCCAGCTTCCGCTCCCGTAGGTAAGCCATGACGACTCCAGCCCAACGGCCTCcgc < 8:248202/147‑1 (MQ=255)aGCGACCACCGTGGCGCCGCGTCCTGCCTATGTGGCTCCACGCCCAGCCTACGTTTCACCGCTAGCCAGCTACAG‑CTATCCCACCAGCAGCTTCTCCAGCTTCCGCTCCCGTAGGTAACCCATGACGACTCCAGCCCAACGGCCTCcgc < 8:248204/149‑1 (MQ=255) gTGGCTCCGCGTCCGGCCTATGTGGCTCCACGCCCAGCCTACGTTGCACCGCGAGCCAGCTACAG‑CTATCGCACCAGCAGCTTCTCCAGCTTCCGCTCCCGTAGGTAAGCCATGACGACTCCAGCCCAACGGCCTCCGCGCCTGAAAg > 4:255136/1‑148 (MQ=255) gCCTATGTGGCTCCACGCCCAGCCTACGTTGCACCGCGAGCCAGCTACAG‑CTATCGCACCAGCAGCTTCTCCAGCTTCCGCTCCCGTAGGTAAGCCATGACGACTCCAGCCCAACGGCCTCCGCGCCTGAAAGAACTGTCCTTGCGCAt > 4:190055/1‑149 (MQ=255) gCTCCACGCCCAGCCTACGTTGCACCGCGAGCCAGCTACAG‑CTATCGCACCAGCAGCTTCTCCAGCTTCCGCTCCCGTAGGTAAGCCATGACGACTCCAGCCCAACGGCCTCCGCGCCTGAAAGAACTGTCCTTgc > 2:49851/1‑136 (MQ=255) gCTCCACGCCCAGCCTACGTTGCACCGCGAGCCAGCTACAG‑CTATCGCACCAGCAGCTTCTCCAGCTTCCGCTCCCGTAGGTAAGCCATGACGACTCCAGCCCAACGGCCTCCGCGCCTGAAAGAACTGTCCTTgc < 1:49851/136‑1 (MQ=255) cACGCCCAGCCTACGTTGCACCGCGAGCCAGCTACAG‑CTATCTAACCAGCAGCTTCTCCAGCTTCCGCTCCCGTAGGTAAGCCATGACGACTCCATCCCAACGGCATCAGAGCCTGAAAGAACTGTACTTGCGAATTGAGCGTGTGGa > 3:142795/1‑148 (MQ=255) cAGCCTACGTTGCACCGCGAGCCAGCTACAGTCTATCGCACCAGCAGCTTCTCCAGCTTCCGCTCCCGTAGGTAAGCCATGACGACTCCAGCCCAACGGCCTCCGCGCCTGAAAGAACTGTCCTTGCGCATTGAGCGTGTGGACAACgg > 1:28245/1‑149 (MQ=255) cAGCCTACGTTGCACCGCGAGCCAGCTACAG‑CTATCGCACCAGCAGCTTCTCCAGCTTCCGCTCCCGTAGGTAAGCCATGACGACTCCAGCCCAACGGCCTCCGCGCCTGAAAGAACTGTCCTTGCGCATTGAGCGTGTGGACAACGGa < 3:255136/149‑1 (MQ=255) cAGCCTACGTTGCACCGCGAGCCAGCTACAG‑CTATCGCACCAGCAGCTTCTCCAGCTTCCGCTCCCGTAGGTAAGCCATGACGACTCCAGCCCAACGGCCTCCGCGCCTGAAAGAACTGTCCTTGCGCATTGAGCGTGTGGACAACGGa < 1:226131/149‑1 (MQ=255) cACCGCGAGCCAGCTACAG‑CTATCGCACCAGCAGCTTCTCCAGCTTCCGCTCCCGTAGGTAAGCCATGACGACTCCAGCCCAACGGCCTCCGCGCCTGAAAGAACTGTCCTTGCGCATTGAGCGTGTGGACAACGGAGTCATCGTCCgg > 8:182913/1‑149 (MQ=255) aCCGCGAGCCAGCTACAG‑CTATCGCACCAGCAGCTTCTCCAGCTTCCGCTCCCGTAGGTAAGCCATGACGACTCCAGCCCAACGGCCTCCGTGCCTGAAAGAACTGTCCTTGCGCATTGAGCGTGTGGACAACGGAGTCATCGTCCGGt > 1:252385/1‑149 (MQ=255) aCCGCGAGCCAGCTACAG‑CTATCGCACCAGCAGCTTCTCCAGCTTCCGCTCCCGTAGGTAAGCCATGACGACTCCAGCCCAACGGCCTCCGTGCCTGAAAGAACTGTCCTTGCGCATTGAGCGTGTGGACAACGGAGTCATCGTCCGGt > 1:248348/1‑149 (MQ=255) ccAGCTACAG‑CTATCGCACCAGCAGCTTCTCCAGCTTCCGCTCCCGTAGGTAAGCCATGACGACTCCAGCCCAACGGCCTCCGCGCCTGAAAGAACTGTCCTTGCGCATTGAGCGTGTGGACAACGGAGTCATCGTCCGGTCCCGAGac < 2:61837/149‑1 (MQ=255) ccAGCTACAG‑CTATCGCACCAGCAGCTTCTCCAGCTTCCGCTCCCGTAGGTAAGCCATGACGACTCCACCCCAACGGCCTCCGCGCCTGAAAGAACTGTCCTTGCGCATTTAGCGTGTGGACAACGGAGTCATCGTCCGGTCCCgaga < 2:45396/148‑1 (MQ=255) ctaTCGCACCAGCAGCTTCTCCAGCTTCCGCTCCCGTAGGTAAGCCATGACGACTCCAGCCCAACGGCCTCCGCGCCTGAAAGAACTGTCCTTGCGCATTGAGCGTGTGGACAACGGAGTCATCGTCCGGTCCCGAGACAACATCCTgg < 7:182913/149‑1 (MQ=255) cTTCTCCAGCTTCCGCTCCCGTAGGTAAGCCATGACGACTCCAGCCCAACGGCCTCCGCGCCTGAAAGAACTGTCCTTGCGCATTGAGCGTGTGGACAACGGAGTCATCGTCCGGTCCCGAGACAACATCCTGGGCCTGGCAAACGAAg < 1:17825/149‑1 (MQ=255) ctcCAGCTTCCGCTCCCGTAGGTAAGCCATGACGACTCCAGCCCAACGGCCTCCGCGCCTGAAAGAACTGTCCTTGCGCATTGAGCGTGTGGACAACGGAGTCATCGTCCGGTCCCGAGACAACATCCTGGGCCTGGCAAACGAAGGt > 2:188986/1‑148 (MQ=255) ctcCAGCTTCCGCTCCCGTAGGTAAGCCATGACGACTCCAGCCCAACGGCCTCCGCGCCTGAAAGAACTGTCCTTGCGCATTGAGCGTGTGGACAACGGAGTCATCGTCCGGTCCCGAGACAACATCCTGGGCCTGGCAAACGAAGGt > 2:208641/1‑148 (MQ=255) cAGCTTCCGCTCCCGTAGGTAAGCCATGACGACTCCAGCCCAACGGCCTCCGCGCCTGAAAGAACTGTCCTTGCGCATTGAGCGTGTGGACAACGGAGTCATCGTCCGGTCCCGAGACAACATCCTGGGCCTGGCAAACGAAGGTGACt > 4:112526/1‑149 (MQ=255) cAGCTTCCGCTCCCGTAGGTAAGCCATGACGACTCCAGCCCAACGGCCTCCGCGCCTGAAAGAACTGTCCTTGCGCATTGAGCGTGTGGACAACGGAGTCATCGTCCGGTCCCGAGACAACATCCTGGGCCTGGCAAACGAAGGTGACt > 2:75112/1‑149 (MQ=255) ttCCGCTCCCGTAGGTAAGCCATGACGACTCCAGCCCAACGGCCTCCGCGCCTGAAAGAACTGTCCTTGCGCATTGAGCGTGTGGACAACGGAGTCATCGTCCGGTCCCGAGACAACATCCt > 3:83143/1‑122 (MQ=255) ttCCGCTCCCGTAGGTAAGCCATGACGACTCCAGCCCAACGGCCTCCGCGCCTGAAAGAACTGTCCTTGCGCATTGAGCGTGTGGACAACGGAGTCATCGTCCGGTCCCGAGACAACATCCt < 4:83143/122‑1 (MQ=255) cagTATGTAAGCCATTACGACTCCATCCCAACTTCCTCCGCGCCTGAAAGAACTTTCCTTGCGCATTTAGCGTGTGTACAACGGAGTCATCGTCCTGTCCCGAGACAACATCCTGGGCCTGGCAAACGAAGTTGACTGCAACGTGTTCa < 4:142795/147‑1 (MQ=255) cGTAGGTAAGCCATGACGACTCCAGCCCAACGGCCTCCGCGCCTGAAAGAACTGTCCTTGCGCATTGAGCGTGTGGACAACGGAGTCATCGTCCGGTCCCGAGACAACATCCTGGGCCTGGCAAACGAAGGTGACTGCAACGTGTTCAc < 1:208641/149‑1 (MQ=255) cGTAGGTAAGCCATGACGACTCCAGCCCAACGGCCTCCGCGCCTGAAAGAACTGTCCTTGCGCATTGAGCGTGTGGACAACGGAGTCATCGTCCGGTCCCGAGACAACATCCTGGGCCTGGCAAACGAAGGTGACTGCAACGTGTTCAc < 1:188986/149‑1 (MQ=255) aaGCCATGACGACTCCAGCCCAACGGCCTCCGTGCCTGAAAGAACTGTCCTTGCGCATTGAGCGTGTGGACAACGGAGTCATCGTCCGGTCCCGAGACAACATCCTGGGCCTGGCAAACGAAGGTGACTGCAACGTGTTCACCAATCg < 2:252385/148‑1 (MQ=255) aaGCCATGACGACTCCAGCCCAACGGCCTCCGTGCCTGAAAGAACTGTCCTTGCGCATTGAGCGTGTGGACAACGGAGTCATCGTCCGGTCCCGAGACAACATCCTGGGCCTGGCAAACGAAGGTGACTGCAACGTGTTCACCAATCg < 2:248348/148‑1 (MQ=255) ccATGACGACTCCAGCCCAACGGCCTCCGCGCCTGAAAGAACTGTCCTTGCGCATTGAGCCTGTGGACAACGGAGTCATCGTCCGGTCCCGAGACAACATCCTGGGCCTGGCAAACGAAGGTTACTGCAACGTGTTCACCAATCGCTAc < 3:185562/149‑1 (MQ=255) ccAGCCCAACGGCCTCCGCGCCTGAAAGAACTGACCTAGCGCATTGAGCGTGTGGACAACGGAGTCATCGTCCGGTCCCGAGACAACATCCTGGGCCTGGCAAACGAAGCTGACTGAAACGTGTTCACCAATCCCTACGAACTGAGCa > 7:65888/1‑148 (MQ=255) cAACGGCCTCCGCGCCTGAAAGAACTGTCCTTGCGCATTGAGCGTGTGGACAACGGAGTCATCGTCCGGTCCCGAGACAACATCCTGGGCCTGGCAAACGAAGGTGACTGCAACGTGTTCACCAATCGCTACGAACTGAGCAACTACCt < 1:75112/149‑1 (MQ=255) aaCGGCCTCCGCGCCTGAAAGAACTGTCCTTGCGCATTGAGCGTGTGGACAACGGAGTCATCGTCCGGTCCCGAGac < 8:211979/77‑1 (MQ=255) cgcgCCTGAAAGAACTGTCCTTGCGCATTGAGCGTGTGGACAACGGAGTCATCGTCCGGTCCCGAGACAAGATCCTGGGACTGGCAAACGAAGGTCACTGCTTCGTGTTCTCCATTCGCTACGAACTTAGCAACTACCTCAACGCtt < 2:28245/147‑1 (MQ=255) cgcgCCTGAAAGAACTGTCCTTGCGCATTGAGCGTGTGGACAACGGAGTCATCGTCCGGTCCCGAGACAACATCCTGGGCCTGGCAAACGAAGGTGACTGCAACGTGTTCACCAATCGCTACGAACTGAGCAACTACCTCAACGCtt < 3:190055/147‑1 (MQ=255) | AGCGACCACCGTGGCTCCGCGTCCGGCCTATGTGGCTCCACGCCCAGCCTACGTTGCACCGCGAGCCAGCTACAG‑CTATCGCACCAGCAGCTTCTCCAGCTTCCGCTCCCGTAGGTAAGCCATGACGACTCCAGCCCAACGGCCTCCGCGCCTGAAAGAACTGTCCTTGCGCATTGAGCGTGTGGACAACGGAGTCATCGTCCGGTCCCGAGACAACATCCTGGGCCTGGCAAACGAAGGTGACTGCAACGTGTTCACCAATCGCTACGAACTGAGCAACTACCTCAACGCTT > NC_002947/2593796‑2594088 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |