| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,650,160 | A→T | 11.3% | intergenic (+99/+35) | PP_2320 → / ← oprI | hypothetical protein/major outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,650,160 | 0 | A | T | 11.3% | 90.3 / 2.7 | 35 | intergenic (+99/+35) | PP_2320/oprI | hypothetical protein/major outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (17/14); new base T (2/2); total (19/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCTTACGGTAGGCTTC > NC_002947/2650013‑2650308 | ccAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACaa < 3:76542/149‑1 (MQ=255) gCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGc < 3:181181/149‑1 (MQ=255) gaaggTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCATGCCTGCCCTTAGTGCATGCCTTTTCGTACCTTCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGcttctt < 6:175307/146‑1 (MQ=255) gATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGcttctt < 8:164291/149‑1 (MQ=255) gAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCTCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGCTTCTTAACAATCCg < 2:133385/149‑1 (MQ=255) aGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGCTTCTTAACAATCCGt < 2:151047/149‑1 (MQ=255) cATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAA‑‑GCCGACCCCACTTGTGGGTCgg > 5:248540/1‑132 (MQ=255) cATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAA‑‑GCCGACCCCACTTGTGGGTCgg < 6:248540/132‑1 (MQ=255) aTTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACTTGTGGGTCGGctt < 8:154336/133‑1 (MQ=255) aTTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACTTGTGGGTCGGctt > 7:154336/1‑133 (MQ=255) cAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCgg > 2:89782/1‑149 (MQ=255) gTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCtt > 1:163574/1‑149 (MQ=255) gCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCa > 6:103310/1‑149 (MQ=255) gCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCAGAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGgc < 3:104425/149‑1 (MQ=255) tGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGCTTCTTAACAATCCGTGa < 2:191003/104‑1 (MQ=255) tGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGCTTCTTAACAATCCGTGa > 1:191003/1‑104 (MQ=255) cTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGGGCCGA‑CCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCt > 1:212685/1‑149 (MQ=255) ttAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCg < 6:15249/80‑1 (MQ=255) ttAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCg > 5:15249/1‑80 (MQ=255) cTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGt > 5:232051/1‑147 (MQ=255) ttttCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAg > 5:9555/1‑149 (MQ=255) ccGCGTTTGCCGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCt > 8:108791/1‑148 (MQ=255) cgcgTTTGCCGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTc > 3:114528/1‑149 (MQ=255) tGCCGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGc < 6:232051/148‑1 (MQ=255) ccGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGa < 7:48349/65‑1 (MQ=255) ccGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGa > 8:48349/1‑65 (MQ=255) ccGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTAc < 7:60367/72‑1 (MQ=255) ccGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTAc > 8:60367/1‑72 (MQ=255) cGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGcttct < 6:13141/48‑1 (MQ=255) tCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTg < 7:51621/81‑1 (MQ=255) tCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTg > 8:51621/1‑81 (MQ=255) aaaGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTg > 4:155729/1‑149 (MQ=255) aaaGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTg > 2:243408/1‑149 (MQ=255) aaaGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTg < 2:212685/149‑1 (MQ=255) aaTAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGcgtcgt > 2:63978/1‑149 (MQ=255) aaaaaa‑‑GCCGA‑CCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAg > 4:176267/1‑149 (MQ=255) acaAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCTTACGGTAGGCt > 1:43799/1‑149 (MQ=255) aaGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTTGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCTTACGGTAGGCTTc < 5:103310/149‑1 (MQ=255) | CCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAA‑‑GCCGA‑CCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCTTACGGTAGGCTTC > NC_002947/2650013‑2650308 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |